Localization of vegetative nucleus and generative cell nuclei in branching pollen tubes of Oenothera hookeri L. grown in vitro

• Autorzy: Strubinska J. , Sniezko R.
• Języki publikacji: EN

Abstrakty

EN

Oenothera hookeri L. pollen germination and pollen tube growth proceed in very similar ways in the pistil and in vitro. A characteristic feature of pollen grain germination is the emergence of one or more pollen tubes from 1, 2 or 3 colpi at the same time. The second specific event is the different way pollen tubes branch during their growth. The present study compares germination and pollen tube growth in fresh and frozen pollen grains. Pollen grain viability was estimated by fluorescein diacetate staining and tested by germination on medium. The localization of nuclear and plastid DNA was detected after DAPI staining. Sperm cell and vegetative nucleus positions were observed in only one of the pollen tube branches and pollen tubes germinating from the same pollen grain. The vegetative nucleus was weakly stained and its fluorescence was dim. The nuclei of sperm cells were very well seen and were found at different distances from the pollen tube tip. After a period of incubation the pollen tube containing sperm cells was always longer than other tubes or branches germinating from the same pollen grain. In some tubes the sperm cell nuclei seemed to change position from one branch to another. Sperm cells probably can move within the pollen tube growing in the ovary to the branch closest to the embryo sac.

Słowa kluczowe

EN

sperm cell; vegetative nucleus; pollen grain; generative cell; pollen tube; Oenothera hookeri; growth; in vitro; nucleus; pollen viability; germination

• Wydawca: -
• Czasopismo: Acta Biologica Cracoviensia. Series Botanica
• Rocznik: 2000
• Tom: 42
• Numer: 2
• Opis fizyczny: p.107-112,fig.

Twórcy

autor

Strubinska J.

• Maria Curie-Sklodowska University, Akademicka 19, 20-033 Lublin, Poland

autor

Sniezko R.

Bibliografia

• Adhikari KN, and Campbell CG. 1998. In vitro germination and viability of buckwheat (Fragopyrum esculentum Moech) pollen. Euphytica 102: 87-92.
• Catcheside DG. 1932. The chromosomes of a new haploid Oenothera. Genetica 15: 68-113.
• Cleland RE. 1972. Oenothera, cytogenetics and evolution. Academic Press, London.
• Derksen J, Rutten T, vanAmstel A, deWin AHN, Doris F, and Steer MW. 1995. Regulation of pollen tube growth. Acta Botanica Neerlandica 44: 93-119.
• De Win AHN, Knuiman B, Pierson ES, Geurts H, Kengen HMP, and Derksen J. 1996. Development and cellular organization of Pinus silvestris pollen tubes. Sexual Plant Reproduction 9: 93-101.
• Fernando DD, and Cass DD. 1997. Developmental assessment of sexual reproduction in Butomus umbellatus (Butomaceae): Male reproductive component. Annals of Botany 80: 449- 456.
• Harte C. 1994. Oenothera-contribution of a plant to biology. Springer-Verlag, Berlin, Heidelberg.
• Heslop-Harrison J, Heslop-Harrison Y, Cresti M, and Ciampolini F. 1991. Ultrastructural features of pollen tubes of Endymion non-scriptus modified by cytochalasin D. Sexual Plant Reproduction 4: 73-80.
• Inagaki MN, and Mujeebkazi A. 1998. Production of polyhaploids of hexaploid wheat using stored pearl millet pollen. Euphytica 100: 253-259.
• Johri BM. 1992. Haustorial role of pollen tubes. Annals of Botany 70: 471-475.
• Kadota A, and Wada M. 1995. Cytoskeletal aspects of nuclear migration during tip-growth in the fern Adiantum protonemal cell. Protoplasma 188: 170-179.
• Kennard JL, and Cleary AL. 1997. Pre-mitotic nuclear migration in subsidiary mother cells of Tradescantia occurs in G1 of the cell cycle and requires F-actin. Cell Motility and the Cytoskeleton 36: 55-67.
• Khatun S, and Flowers TJ. 1995. The estimation of pollen viability in rice. Journal of Experimental Botany 46: 151- 154.
• Liu B, and Palevitz BA. 1996. Localization of kinesin-like protein in generative cells of tobacco. Protoplasma 195: 78-89.
• Miyake T, Kuroiwa H, and Kuroiwa T. 1995. Differential mechanisms of movement between a generative cell and vegetative nucleus in pollen tubes of Nicotiana tabacum as revealed by additions of colchicine and nonanoic acid. Sexual Plant Reproduction 8: 228-230.
• Pierson ES, and Cresti M. 1992. Cytoskeleton and cytoplasmic organization of pollen and pollen tubes. International Review of Cytology 140: 73-125.
• Renner O. 1917. Versuche ber die gemetische Konstitution der Oenotheren. Zeitser Indukt Abstammungs Vererbungsl 18: 121-294.
• Rodrigues GS, Ma TH, Pimentel D. 1997. Tradescantia bioassays as monitoring systems for environmental mutagenesis: A review. Critical Reviews in Plant Science 16: 325-359.
• Sodmergen, Kuroiwa H, Nagata N, Qian ZH, Liu H, Guan XL, and Kuroiwa T. 1997. Quantitative study of organelle nucleoids during the second pollen grain mitosis in Oenothera biennis. Protoplasma 198: 66-72.
• Southworth D. 1996. Gametes and fertilization in flowering plants. Current Topics in Developmental Biology 34: 259- 279.
• Steer MW, and Steer JM. 1989. Tansley Review No.16 Pollen tube tip growth. New Phytologyst 111: 323-358.
• Śnieżko R. 1996. Pollen tube branching in the ovary of five species of Oenothera. Acta Societatis Botanicorum Poloniae 65: 111- 116.
• Śnieżko R. 1997. Postulated interaction between branching pollen tubes and ovules in Oenothera hookeri de Vries (Onagraceae). Journal of Plant Research 110: 411-416.
• Śnieżko R, Nowakowska A, and Budkowska B. 1995. Pollination and fertilization in 6 species of Oenothera. Abstracts of VII Conference of Plant Embryologists of Czech Republic, Slovakia and Poland, Lublin, 53.
• Tanaka K. 1956. The pollen germination and pollen tube development in Pinus densiflora Sieb. et Zuncc. II. The tube growth and tube nucleus. Science Reports of the Tohoku University Series 4 B 22: 219-224.
• Tian HQ, and Russel SD. 1998. The fusion of sperm cells and the function of male germ unit (MGU) of tobacco (Nicotiana tabacum L.). Sexual Plant Reproduction 11: 171-176.
• Tian HQ, Zhang Z, and Russel SD. 1998. Isolation of the male germ unit: organization and function in tobacco (Nicotiana tabacum L.). Plant Cell Reports 18: 143-147.
• Wilms HJ. 1974. Branching of pollen tube in spinach. In: Linkens HF [ed], Fertilization in higher plants, 155-160. North Holland Publ. Co., Amsterdam.