Rapid separation of tyrosine - specific tRNA from white lupin

• Autorzy: Barciszewska M. Z. , Krajewski J. , Gawronska I. , Barciszewski J.
• Języki publikacji: EN

Abstrakty

EN

During Isolation of total rilmnucleic acids from white lupin (Lupinus albus) and their subsequent separation by 10% polyacrylamide gel electrophoresis, a fast migrating RNA band is very well separated. The nucleotide sequence analysis of 76 nucleotide long sequence with many modified nucleosides was found to be identical with that of tyrosine specific tRNA of yellow lupin seeds (Lupinus luteus) and wheat germ (Trtiicum aestivum). Also this tRNATyr is identical with plant amber suppressor tRNA. 'I'he presented approach offers a very rapid method of purification of plant tRNA with UAG suppressor activity.

Słowa kluczowe

EN

white lupin; tRNA; tyrosine; lupin

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1992
• Tom: 39
• Numer: 2
• Opis fizyczny: p.223-226,fig.

Twórcy

autor

Barciszewska M. Z.

• Institute of Bioorganic Chemistry, Noskowskiego 12, 61-704 Poznan, Poland

autor

Krajewski J.

autor

Gawronska I.

autor

Barciszewski J.

Bibliografia

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• 4. Barciszewski, J., Barciszewska, M , Suter, B. & Kubli. E. (1985) Hani Sei.. 40. 193 - 196.
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