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Przebadano w automatycznym systemie BacT/Alert próbki krwi pobrane od pacjentów hospitalizowanych w oddziałach zabiegowych i zachowawczych SP CSK AM w Warszawie. Próbki krwi pobierano od chorych bezpośrednio z żyły lub za pomocą cewnika naczyniowego. Wyhodowano 38 szczepów grzybów drożdżopodobnych z krwi obwodowej i 20 szczepów z krwi pobranej przy użyciu cewnika naczyniowego. Gatunkami dominującymi były: C. albicans, C. glabrata i C. parapsilosis.
The aim of the study was the analysis of frequency of yeast-like fungi as etiological agents of fungemias in patients hospitalized in operative and conservative wards of Medical Academy Central Clinical Hospital in Warsaw in 1998-1999. Peripheral blood samples and collected from vascular catheters were incubated in BacT/Alert system(Organon Teknika, USA). Positive blood samples were inoculated on Sabouraud medium with chloramphenicol (bioMerieux, France) (the time of cultivation from 48 h to 7 days at 30 C) and on chromogenic medium BBL® CHROMagar™ Candida (Becton Dickinson, USA). Fungal strains were identified by standard mycological procedures using ID 32 C strips (ATB system, bioMerieux, France) and tests of Sanofi Diagnostics Pasteur (France). The total number of positive blood cultures was 1724. Fifty eight fungal strains were isolated from blood samples (3.36%). Strains belonged to 4 genera: Candida (55), Trichosporon (1), Saccharomyces (1) and Pichia (1). Thirty eight fungal strains were isolated from peripheral blood samples. Forty seven fungal strains were cultured from patients hospitalized in operative wards. Among fungi isolated from peripheral blood samples C. albicans (10), C. glabrata (9), and C. parapsilosis (5) strains dominated. From blood samples collected from vascular catheters most often C. albicans (7), C. glabrata (4) and C. parapsilosis (3) were isolated.The aim of the study was the analysis of frequency of yeast-like fungi as etiological agents of fungemias in patients hospitalized in operative and conservative wards of Medical Academy Central Clinical Hospital in Warsaw in 1998-1999. Peripheral blood samples and collected from vascular catheters were incubated in BacT/Alert system(Organon Teknika, USA). Positive blood samples were inoculated on Sabouraud medium with chloramphenicol (bioMerieux, France) (the time of cultivation from 48 h to 7 days at 30 C) and on chromogenic medium BBL® CHROMagar™ Candida (Becton Dickinson, USA). Fungal strains were identified by standard mycological procedures using ID 32 C strips (ATB system, bioMerieux, France) and tests of Sanofi Diagnostics Pasteur (France). The total number of positive blood cultures was 1724. Fifty eight fungal strains were isolated from blood samples (3.36%). Strains belonged to 4 genera: Candida (55), Trichosporon (1), Saccharomyces (1) and Pichia (1). Thirty eight fungal strains were isolated from peripheral blood samples. Forty seven fungal strains were cultured from patients hospitalized in operative wards. Among fungi isolated from peripheral blood samples C. albicans (10), C. glabrata (9), and C. parapsilosis (5) strains dominated. From blood samples collected from vascular catheters most often C. albicans (7), C. glabrata (4) and C. parapsilosis (3) were isolated.
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Czasopismo
Rocznik
Tom
Numer
Strony
167-171
Opis fizyczny
s.167-171,tab.,bibliogr.
Twórcy
autor
- Akademia Medyczna, ul.T.Chalubinskiego 5, 02-004 Warszawa
autor
autor
autor
autor
autor
autor
Bibliografia
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- 2. Bannister BA, Begg NT, Gillespie SH. Choroby Zakaźne. Wydawnictwo Medyczne Urban & Partner. Wydanie I polskie pod redakcją J. Juszczyka, Wrocław 1998.
- 3. Blot S, Vanderwoude K, Hoste E i inni. Outcome in critically ill patients with candidal fungaemia: Candida albicans vs. Candida glabrata. J Hosp Infect 2001; 47: 308-13.
- 4. Dzierżanowska D, Jeljaszewicz J. Zakażenia szpitalne. α-medica press. Wydanie I, Bielsko-Biała 1999.
- 5. Elewski BE. Grzybicze zakażenia skóry. α-medica press. Wydanie I, Bielsko-Biała 2000.
- 6. Groll AH, Walsh TJ. Uncommon opportunistic fungi: new nosocomial threats. Clin Microbiol Infect 2001; 7(Suppl. 2): 8-24.
- 7. Hadfield TL, Smith MB, Winn RE i inni. Mycoses caused by Candida lusitaniae. Rev Infect Dis 1987; 9: 1006-12.
- 8. Hazen KC. New and emerging yeast pathogens. Clin Microbiol Rev 1995; 8: 462-78.
- 9. Pawlik B, Macura AB. Diagnostyka laboratoryjna w mikologii. W: Zarys mikologii lekarskiej. Red. E. Baran, Volumed, Wroclaw 1998, 541-72.
- 10. Pfaller MA, Houston A, Coffman S. Application of CHROMagar Candida for rapid screening of clinical specimens for Candida albicans, Candida tropicalis, Candida krusei, and Candida (Torulopsis) glabrata. J Clin Microbiol 1996; 34: 58-61.
- 11. Pfaller MA, Wenzel RP. Impact of changing epidemiology of fungal infections in the 1990s. Eur J Clin Microbiol Infect Dis 1992; 11: 287-91.
- 12. Procop G, Cockerill FR III, Vetter EA i inni. Performance of five agar media for recovery of fungi isolator blood cultures. J Clin Microbiol 2000; 38: 3827-29.
- 13. Snigh N. Changing spectrum of invasive candidiasis and its therapeutic implications. Clin Microbiol Infect 2001; 7(Suppl. 2): 1-7.
- 14. Uzun O, Anaissie EJ. Antifungal prophylaxis in patients with hematologic malignancies: a reappraisal. Blood 1995; 86: 2063-72.
- 15. Walsh TJ, Finberg RW, Arndt C i inni. Liposomal amphotericin B for empirical therapy in patients with persistent fever and neutropenia. N Engl J Med 1999; 340: 767-71.
- 16. Walsh TJ, Pizzo A. Treatment of systemic fungal infections: recent progress and current problems. CurrJ Clin Microbiol Infect Dis 1988; 7: 460-75.
- 17. Warnock DW. Fungal infections in neutropenia: current problems and chemotherapeutic control. J Antimicrob Chemoter 1998; 41(Suppl. D): 95-105.
- 18. Warren NG, Hanzen KC. Candida, Cryptococcus and other yeasts of medical importance. W: Manual of Clinical Microbiology. Red. P. R. Murray i inni, ASM Press, Waszyngton 1995, 723-37.
- 19. Wingard JR. Importance of Candida species other than C. albicans as pathogens in oncology patients. Clin Infect. Dis 1995; 20: 15-25.
- 20. Wingard JR, Merz WG, Rinaldi MG i inni. Increase in Candida krusei infection among patients with bone marrow transplantation and neutropenia treated prophylactically with fluconazole. N Engl J Med 1991; 325: 1274-77.
- 21. Złotorowicz M. Zakażenia grzybicze u chorych leczonych w oddziale intensywnej terapii. W: Zakażenia w intensywnej terapii. Wydawnictwo Medyczne Urban & Partner. Wydanie I, Wrocław 2000, 105-13.
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Bibliografia
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