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The aim of work was to examine the influence of lactobionic acid, gluconic acid, delta-gluconolactone, lactulose and sorbitol on the production of cellulases by mutant strain Trichoderma reesei M-7 during batch and continuous cultivation. Positive results were achieved in the presence of lactobionic acid and lactulose as inducers. In both cases the highest inductive effect of cellulase production was observed in the presence of 1% mixtures of above mentioned substrates at the ratio 1:1 (0.5% : 0.5%) with lactose. (about 20% higher than that observed in the presence of 1% of lactose) The induction mechanism of lactobionic acid and lactulose may be attributed by slow rate of their utilisation (slower than lactose). Explanation of the inductive mechanism of lactose, lactobionic acid and lactulose on cellulases production by Trichoderma reesei demands further investigations among other with using a purified beta-galactosidase enzyme.
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- Agricultural University of Lublin, Skromna 8, 20-950 Lublin, Poland
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Bibliografia
- Bruchman E.E., Graf H., Saad A.A., and Schrenk D., 1978. Zur Gewinnung hochaktiver cellulasepreparate und optimierung der enzymatischen cellulosehydrolyse [Production of active cellulase preparations and optimisation of enzymatic cellulose hydrolysis]. II. Chem. Ztg., 102, 154-155.
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- Ghose T.K., 1987. Measurement of cellulase activities. Pure Appl. Chem., 59, 257-268.
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- Lovrien R.E., Gusek T., Hart B., 1985. Cellulase and protease specific activities of cemmercialy available cellulase preparations. J. Appl. Biochem., 7, 258-272.
- Lowry O.H., Rosenbourh N.J., Farr R.L., Rendel R.J., 1951. Protein measurements with the folin phenol reagent. J. Biol. Chem.,193, 265-275.
- Lunt M.R., Kent P., 1960. A chitinase system from Carcinus meamas. Biochim. Biophys. Acta, 44, 371-373.
- Mandels M., Andreotti R., Roche C., 1976. Measurement of saccharifying cellulase. Biochim. Bioeng. Symp., 6, 21-23.
- Mandels M., Weber J., 1969. The production of cellulase: [In:] Cellulase and their application. Eds. G. J. Hajny and E. T. Reese. Adv.Chem.Ser., 95, 391-413.
- Miller G.L., 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugars. Anal. Chem., 31, 426-428.
- Szakmary K., Wotawa A., Kubicek C.P., 1991. Origin oxidized cellulose degradation products and mechanism of their promotion of cellobiohydrolase I biosynthesis in Trichoderma reesei. J. Gen. Microbiol., 137, 2873-2878.
- Targoński Z., 1991. Biosynteza celulaz, ksylanaz i enzymów litycznych przez Trichoderma reesei QM 9414 i Trichoderma viridae F-19 [Biosyntesis of cellulases and ksylanases and lytic enzymes by the use of Trichoderma reesei QM 9414 i Trichoderma viridae F-19]. Biotechnologia, 2 (12): 50-58 [in Polish].
- Targoński Z., Szajer S., 1977. Synthesis of cellulase by Fusarium sp. in different culture conditions. Acta Microbiol., 269 (3): 273-279.
- Vaheri M., 1982 a. Oxidation as a part of degradation of crystaline cellulose by Trichoderma reesei. J. Appl. Biochem., 4, 356-363.
- Vaheri M., 1982 b. Acidic degradation products of cellulose during enzymatic hydrolysis by Trichoderma reesei. J. Appl. Biochem., 4, 153-160.
- Vaheri M., 1983. Formation of oxidative activity for the initial degradation of crystaline cellulose by Trichoderma reesei. J. Appl. Biochem., 5, 66-74.
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Bibliografia
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