Investigations on cryptosporidiosis in humans and livestock from the same localities

• Autorzy: Majewska A C , Kasprzak W. , Werner A. , Kozakiewicz B.
• Języki publikacji: EN

Abstrakty

EN

The objective of this study was to screen humans and farm animals from the same localities for Cryptosporidium infections. Coprological examinations using Ziehl-Neelsen staining and enzyme immunoassay included 68 faecal specimens from calves, 16 from pigs, 86 from humans (76 from children, 4 from farmers, 6 from laboratory workers) and 4 samples of pigsty litter. Cryptosporidium infections were found in 60 calves and 1 piglet. Morphometric characteristics of the oocysts revealed that most infections were caused by C. parvum while only 2 calves were infected with C. muris. C. parvum oocysts were found in litter materials on two small privately-owned farms. The results of the study showed that despite the possible high level of exposure to C. parvum oocysts excreted by infected calves bred in the cooperative farm no children from the same locality were infected. The children were also free of other intestinal protozoa. Similar results were obtained in the study performed on two small privately-owned farms. Although one piglet was infected with C. parvum on one of the farms and oocysts were found in litter materials on both farms, no farmers were infected.

Słowa kluczowe

EN

infection; investigation; locality; man; cryptosporidiosis; epidemiology; Protozoa; Cryptosporidium; livestock

• Wydawca: -
• Czasopismo: Acta Parasitologica
• Rocznik: 1999
• Tom: 44
• Numer: 4
• Opis fizyczny: p.211-214

Twórcy

autor

Majewska A C

• Karol Marcinkowski University of Medical Sciences, Fredry 10, 61-701 Poznan, Poland

autor

Kasprzak W.

autor

Werner A.

autor

Kozakiewicz B.

Bibliografia

• Anonymous. 1991. Basic laboratory methods in medical parasitology. World Health Organization, Geneva, 18.
• Bajer A., Bednarska M., Siński E. 1997. Wildlife rodents from different habitats as a reservoir for Cryptosporidium parvum. Acta Parasitologica, 42, 192-194.
• Bednarska M., Bajer A., Siński E. 1998. Calves as a potential reservoir of Cryptosporidium parvum and Giardia sp. Annals of Agricultural and Environmental Medicine, 5, 135-138.
• Bornay-Llinares F. J., Da Silva A. J., Moura I. N. S., Myjak P.. Pietkiewicz H., Kruminis-Łozowska W., Graczyk T. K., Pieniazek N. J. 1999. Identification of Cryptosporidium felis in a cow by morphologic and molecular methods. Applied and Environmental Microbiology, 65, 1455-1458.
• Casemore D. P., Wright S. E., Coop R. L. 1997. Cryptosporidiosis - human and animal epidemiology. In: Cryptosporidium and cryptosporidiosis (Ed. R. Fayer). CRC Press, Boca Raton, FL, 65-92.
• Chalmers R. M., Strudee A. P., Bull S. A., Miller A., Wright S. E. 1997. The prevalence of Cryptosporidium parvum, and C. muris in Mus domesticus, Apodemus sylvaticus and Clethrionomys glareolus in an agricultural system. Parasitology Research, 83, 478-482.
• Chappell C. L., Okhuysen P. C., Sterling C. R., DuPont H. L.1996. Cryptosporidium parvum: intensity of infection and oocysts excretion patterns in healthy volunteers. Journal of Infectious Diseases, 173, 232-236.
• Fayer R., Speer C. A., Dubey J. P. 1997. The general biology of Cryptosporidium. In: Cryptosporidium and cryptosporidiosis (Ed. R. Fayer). CRC Press, Boca Raton, FL, 1-41.
• Ignatius R., Eisenblätter M., Regnath T., Mansmann U., Futh U., Hahn H., Wagner J. 1997. Efficacy of different methods for detection of low Cryptosporidium parvum oocyst numbers or antigen concentrations in stool specimens. European Journal of Clinical Microbiology and Infectious Diseases, 16, 732-736.
• Kozakiewicz B., Maszewska I. 1988. Ekstensywność inwazji Cryptosporidium sp. u cieląt z objawami biegunki w gospodarstwach wielkostadnych. Medycyna Weterynaryjna, 44, 404-407.
• Majewska A. C., Kasprzak W., Werner A. 1997. Prevalence of Cryptosporidium in mammals housed in Poznań Zoological Garden, Poland. Acta Parasitologica, 42, 195-198.
• Majewska A. C., Werner A., Sulima P. 1998. Prevalence of cryptosporidiosis in livestock in Wielkopolska region. 18th Congress of the Polish Parasitological Society, 9-11 September, 1998, Olsztyn, Poland. Abstracts. Wiadomości Parazytologiczne, 44, 471.
• Millard P. S., Gensheimer K. F., Addiss D. G., Sosin D. M., Beckett G. A., Houck-Jankoski A., Hudson A. 1994. An outbreak of cryptosporidiosis from fresh-pressed apple cider. Journal of the American Medical Association, 272, 1592-1596.
• Morgan U. M., Sargent K. D., Deplazes P., Forbes D. A., Spano F., Hertzberg H., Elliot A., Thompson R. C. A. 1998. Molecular characterization of Cryptosporidium from various hosts. Parasitology, 117, 31-37.
• O'Donoghue P. J. 1995. Cryptosporidium and cryptosporidiosis in man and animals. International Journal for Parasitology, 25, 139-195.
• Peng M. M., Xiao L., Freeman A. R., Arrowood M. J., Escalante A. A., Weltman A. C., Ong C. S. L., Mackenzie W. R., Lal A. A., Beard C. B. 1997. Genetic polymorphism among Cryptosporidium parvum isolates: evidence of two distinct human transmission cycles. Emerging Infectious Diseases, 3, 567-573.
• Pieniazek N. L., Bornay-Llinares F. J., Slemenda S. B., Da Silva A. J., Moura I. N. S., Arrowood M. J., Ditrich O., Addiss D. G. 1999. New Cryptosporidium genotypes in HIV-infected persons. Emerging Infectious Diseases, 5, 444-449.
• Pohjola S., Jokipii A. M. M., Jokipii L. 1986. Sporadic cryptosporidiosis in a rural population is asymptomatic and associated with contact to cattle. Acta Veterinaria Scandinavica, 27, 91-102.
• Smith H. V., Rose J. B. 1998. Waterborne cryptosporidiosis: current status. Parasitology Today, 14, 14-22.
• Weber R., Bryan R. T., Bishop H. S., Wahlquist S. P., Sullivan J. J., Juranek D. D. 1991. Threshold of detection of Cryptosporidium oocysts in human stool samples: evidence for low sensitivity of current diagnostic methods. Journal of Clinical Microbiology, 29, 1323-1327.