The use of tissue culture methods in interspecific hybridization within the genus Trifolium L.

• Autorzy: Przywara L. , Pirog H. , Konieczny R. , Jach M.
• Języki publikacji: EN

Abstrakty

EN

An attempt was made to produce interspecific hybrids among Trifolium species. In situ pollination followed by in ovulo embryo culture and culture of the excised embryos, and in vitro pollination have been applied. 21 embryos were obtained from 636 in situ pollinations. 13 embryos were green and showed normal development. It was found that these embryos were produced in the result of self-fertilization. The remaining 8 embryos were albino or chlorotic. The albino embryos originated from the combinations: T. hybridum x T. montanum (1 embryo); T. repens x T. resupinatum (6 embryos) and T. resupinatum x T. montanum (1 embryo). All the albino embryos produced were cultured on EG medium. The growth and development of embryos were abnormal. Most of them did not grow and died within two or three weeks of culture. Only a single embryo from the cross T. hybridum x T. montanum survived and after transferring to MS medium supplemented with 0.5 mg/l NAA and 2 mg/l 2iP produced callus. It is supposed that albino embryos were hybrids. In vitro pollination of T. repens pistils with T. nigrescens pollen resulted in the formation of 27 hybrid embryos (6 embryos were produced in reciprocal cross) from which 11 hybrid plants were raised. Hybrid embryos not growing were passaged to the MS medium supplemented with 0.5 mg/l NAA and 2 mg/l 2iP to induce callus. Plants were regenerated from the calli via organogenesis.

Słowa kluczowe

EN

in ovulo embryo culture; in vitro pollination; interspecific hybridization; in situ pollination; tissue culture method; Trifolium

• Wydawca: -
• Czasopismo: Acta Biologica Cracoviensia. Series Botanica
• Rocznik: 1996
• Tom: 38
• Opis fizyczny: p.53-65,fig.

Twórcy

autor

Przywara L.

• Jagiellonian University, Grodzka 52, 31-044 Krakow, Poland

autor

Pirog H.

autor

Konieczny R.

autor

Jach M.

Bibliografia

• Ahuja P. S., Lu D. Y., Cocking E. C, and Davey M. R. 1983. An assessment of the culture capabilities of Trifolium repens (white clover) and Onobrychis viciifolia Scop. (sainfoin) mesophyll protoplasts. Plant Cell Reports 2, 269-272.
• Bhojwani S. S., Mullins K. and Cohen D. 1984. Intravarietal variation for in vitro plant regeneration in the genus Trifolium. Euphytica 33, 915-921.
• Bhojwani S. S. and White D. W. R. 1982. Mesophyll protoplasts of white clover isolation, culture and organogenesis. Plant Science Letters 26, 265-271.
• Brewbaker J. L. and Keim W. F. 1953. A fertile interspecific hybrid in Tri folium (4n T. repens L. x 4n T. nigrescens Viv.). Amer. Nat. 87, 323-326.
• Cleveland R. W. 1985. Reproductive cycle and cytogenetics, pp. 71-110. In Clover Science and Technology, 1 ed. N. L. Taylor, Agronomy Monograph No 25, Madison, Wisconsin, USA
• Douglas G. C. and Connolly V. 1989. Self fertilization and seed set in Trifolium repens L. by in situ and in vitro pollination. Theor. and Applied Genetics 77, 71-75.
• Gresshoff P. M. 1980. In vitro culture of white clover: callus, suspension, protoplast culture and plant regeneration. Bot. Gaz. 141, 157-164.
• Grosser J. W. 1985. Approaches to interspecific hybridization of red clover with perennial Trifolium species via embryo rescue and somatic cell fusion. Dissertation Abstracts Int. Vol. 45, No 09.
• Honkanen J., Ryoppy P. and Tigerstedt P. M. A. 1986. Protoplast culture and fusion of red and alsike clover. In: Genetic manipulation in plant breeding. Eds: Horn W., Jensen C. J., Odenbach W., Schieder O. Proc. Int. Symp. EUCARPIA, Sept. 8-13, 1985, Berlin (West Germany), De Gruyter, Berlin, pp. 685-687.
• Honkanen J., Ryoppy P. and Tigerstedt P. M. A. 1988. Towards somatic hybridization in clovers. Proc. Int. Symp. Genetic Manipulation in Crops. Oct. 22-26, 1984, Beijing, China (1RRI), 413.
• Honkanen J. and Ryoppy P. 1989. Somatic hybridization in Trifolium. In: Biotechnology in Agriculture and Forestry 8. Plant Protoplast and Genetic Engineering I. Springer Verlag, 397-409.
• Kao K. N. and Michalyuk M. R. 1975. Nutritional requirements for growth of Vicia hajastana cells and protoplasts in a very low density in liquid media. Planta 126, 105-110.
• Kazimierski T. and Kazimierska E. M. 1970. Badania mieszańców w rodzaju Trifolium L. III. Cechy morfologiczne i cytogenetyka mieszańca Trifolium repens L. x Trifolium nigrescens Viv. Acta Soc. Bot. Polon. 39, 565-591.
• Kazimierski T. and Kazimierska E. M. 1973. Badania mieszańców w rodzaju Trifolium L. IV. Cytogenetyka mieszańca Trifolium repens L. x T. isthmocarpum Brot. Acta Soc. Bot. Pol. 41, 129-147.
• Keyes G. J., Collins G. B. and Taylor N. L. 1980. Genetic variation in tissue cultures of red clover. Theoretical and Applied Genetics, 58, 265-271.
• Konieczny R. 1995. Plant regeneration in callus culture of Trifolium nigrescens Viv. Acta Biol. Cracov., Ser. Bot. 37, 47-52.
• Kowalska-Rębilas A. and Przywara L. 1995. Krytyczne punkty w procedurze otrzymywania protoplastów z Trifolium repens. In: Zastosowanie kultur in vitro w fizjologii roślin. Eds: Dubert F. and Skoczowski A. PAN, Kraków
• DeLautour G., Jones W. T. and Ross M. D. 1978. Production of inter-specific hybrids in Lotus aided by endosperm transplants. New Zealand Jour. Bot. 16, 61-68.
• Murashige T. and Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physi. Plant. 15, 473-497.
• Pandey K. K. 1957. A self-compatible hybrid from a cross between two self-incompatible species in Trifolium. Journ. of Heredity 48, 278-281.
• Pandey K. K., Grant J. E. and Williams E. G. 1987. Interspecific hybridization between Trifolium repens and T. uniflorum. Australian Jour. Bot. 35, 171-182.
• Phillips G. C. and Collins G. B. 1979. In vitro culture of selected legumes and plant regeneration from callus cultures of red clover. Crop Sci. 19, 59-64.
• Przywara L. and Konieczny R. 1993. An attempt to cross Trifolium repens L. with T. xerocephalum Fenzl. Fragm. Flor. et Geobot. 38, 69-74.
• Przywara L., White D. W. R., Sanders P. M. and Maher D. 1989. Interspecific hybridization of Trifolium repens with T. hybridum using in ovulo embryo and embryo culture. Ann Bot. 64, 613-624.
• Quesenberry K. H. and Taylor N. L. 1976. Interspecific hybridization in Trifolium L. sect. Trifolium Zoh. I. Diploid hybrids among T. alpestre L., T. rubens L., T. heldrechianum Hausskn., and T. noricum Wulf. Crop Science 16, 382-386.
• Richards K. W. and Rupert E. 1980. In vitro fertilization and seed development in Trifolium. In Vitro 16, 925-931.
• Rupert E. A., Seo A. and Richards K. W. 1979. Trifolium species hybrids obtained from embryo-callus tissue cultures. Agron. Abstracts 75.
• Rupert E. A. and Collins G. B. 1985. Tissue culture, In: Clover Science and Technology, led. Agronomy Monograph No 25, Ed. Taylor N. L. Madison, Wisconsin, USA
• Rybczyński J. J. 1991. Regeneracja roślin w kulturach in vitro niektórych gatunków z rodziny Fabaceae. Wydawnictwa SGGW Warszawa, 142, 7-64.
• Shillito R. D., Paszkowski J., and Potrykus I. 1983. Culture in agarose improves protoplast plating and permits division in otherwise unresponsive systems. In: Protoplasts 1983. Poster Proc. 6th Int. Protoplast Symp. Basel. Aug. 12-16, 1983, Eds: Potrykus I., Harms C. T., Hinnen A., Hutter R., King P. J., Shillito R. D. Birkhauser, Basel.
• Tilton V. R. and Russell S. H. 1983. In vitro pollination and fertilization of soybean Glycine max (L.) Merr. (Leguminosae). In: Pollen biology and implications for plant breeding. Eds: Mulcahy D. L. and Ottaviano E. Elsevier, Amsterdam, 281-286.
• Webb K. J., Woddcock S., Pike L. S. and Dale P. J. 1986. Plant regeneration in the forage legumes. In: Plant tissue culture and its agricultural application. Eds: Withers L. A. and Alderson P. G. London, Butterworths, 99-104.
• Webb K. J., Woodcock S. and Chamberlain D. A. 1987. Plant regeneration from protoplasts of Trifolium repens and Lotus corniculatus. Plant Breed. 98, 111-118.
• White D. W. R. 1983. Plant regeneration from mesophyll protoplasts of white clover {Trifolium repens) In: Protoplasts 1983. 6th Int. Protoplast Symp. Basel. Aug. 12-16, 1983. Eds: Potrykus I., Harms C. T., Hinnen A., Hutter R., King P. J., Shillito R. D. Birkhauser, Basel, pp. 61-62.
• Williams E. G. 1978. A hybrid between Trifolium repens and T. ambiguum obtained with the aid of embryo culture. New Zealand Jour. Bot. 16, 499-506.
• Williams E. O. 1987. Interspecific hybridization in pasture legumes. Plant Breed. Rev. 5, 237-305.
• Williams E. O. and deLautour G. 1980. The use of embryo culture with transplanted nurse endosperm for the production of interspecific hybrids in pasture legumes. Bot. Gaz. 141, 252-257.
• Yamada T. 1989. Selection of a highly-regenerative genotyp of white clover (Trifolium repens) and plant regeneration from protoplasts derived from this genotype. Euphytica 44, 181-186.
• Yamada T. and Fukuoka H. 1985. Application of ovule culture to interspecific hybridization between Trifolium repens and T. ambiguum. Proceedings of the XVth Internat. Grasslands Congress, pp. 241-243. Yamada T. and Higuchi S. 1990. In vitro culture of genus Trifolium germplasm and plant regeneration. Journ. of Jap. Soc. of Grasslands Sci. 36, 47-55.
• Zenkteler M. 1990. In vitro fertilization and wide hybridization in higher plants. Critical Reviews in Plant Sciences 9, 267-279.
• Zenkteler M. 1991. Ovule culture and test tube fertilization. Med. Fac. Landbouww. Rijksuniv. Gent, 56 (4a), 1403-1410.