Zastosowanie metod biologii molekularnej w rozpoznawaniu zakazen grzybiczych

• Autorzy: Garczewska B. , Dzierzanowska D.
• Języki publikacji: PL

Abstrakty

PL

Badania przeprowadzono u 48 chorych hospitalizowanych w latach 1998-1999 z podejrzeniem uogólnionego bądź układowego zakażenia grzybiczego. Próbki uzyskanego materiału klinicznego badano klasycznymi metodami mikrobiologicznymi oraz zastosowano technikę PCR. Wykazano i potwierdzono możliwość wykorzystania technik biologii molekularnej do szybkiego wykrywania DNA Candida albicans w próbkach materiału klinicznego oraz do monitorowania leczenia przeciwgrzybiczego.

EN

A total of 130 various clinical materials taken from 48 children with suspected systemic fungal infection were used for the study. Clinical samples were tested by use of classical mycological procedures well as by use of molecular technique (PCR assay). The fragments of 125-bp (EO3) and 317bp (HSP) specific for C.albicans were used for amplification. Fifty seven samples (48%) were positive for Candida albicans and eighty four (68%) by use of PCR. It should be stressed that 4 blood samples, 21 urine samples and 5 other samples were positive by use of molecular technique, only. PCR is sensitive and rapid method for detection and identification of Candida albicans from clinical materials of children with fungal infection. This technique can be applied for monitoring presence of fungal DNA in tested samples during antifungal therapy.

Słowa kluczowe

PL

Candida albicans; kandydoza; diagnostyka mikrobiologiczna; szpitale; choroby czlowieka; zakazenia grzybicze; lancuchowa reakcja polimerazy; biologia molekularna

• Wydawca: -
• Czasopismo: Medycyna Doświadczalna i Mikrobiologia
• Rocznik: 2002
• Tom: 54
• Numer: 2
• Strony: 157-165
• Opis fizyczny: s.157-165,tab.,fot.,bibliogr.

Twórcy

autor

Garczewska B.

• Centrum Zdrowia Dziecka, Aleje Dzieci Polskich 20, 04-736 Warszawa

autor

Dzierzanowska D.

Bibliografia

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