Lamium album extracts express free radical scavenging and cytotoxic activities

• Autorzy: Paduch R , Matysik G. , Wojcik-Kosior M. , Kandefer-Szerszen M. , Skalska-Kaminska A. , Nowak-Kryska M. , Niedziela P.
• Języki publikacji: EN

Abstrakty

EN

Lamium album, commonly known as “white dead nettle,” is a perennial herb widely used in folk medicine. The present paper presents the toxic, anti-proliferative, and free radical (DPPH˙) scavenging activities of methanol and ethyl acetate extracts of that plant. In order to determine the biologically active compounds, the plant extracts were separated by high performance thin layer chromatography (HPTLC) on silica gel Si 60 F254 and high performance liquid chromatography (HPLC) combined with densitometry. Methanol extract was rich mainly with flavonoids and phenolic acids. Ethyl acetate extract contained mainly triterpenes. Both extracts showed no toxic effects against normal human skin fibroblasts (HSF) in the range of applied concentrations (25–225 μg/ml). Anti-proliferative activity revealed that methanol extract expressed lower inhibitory properties than ethyl acetate one. The MTT test was, however, less sensitive than Neutral Red (NR) assay. Ethyl acetate extract did not exhibit DPPH˙ radical scavenging activity. Methanol extract reduced the radical of about 29% at the highest applied concentration (225 μg/ml). Both extracts slightly influenced cellular cytoskeleton organization and amount, and size of agyrophilic nucleolar organizer regions (AgNOR) protein deposits. These findings suggest that extracts of Lamium album exhibit potential usefulness in preparation of new natural formulations.

• Wydawca: -
• Czasopismo: Polish Journal of Environmental Studies
• Rocznik: 2008
• Tom: 17
• Numer: 4
• Strony: 569-580
• Opis fizyczny: p.569-580,fig.,ref.

Twórcy

autor

Paduch R

• Maria Curie-Sklodowska University, Akademicka 19, 20-033 Lublin, Poland

autor

Matysik G.

autor

Wojcik-Kosior M.

autor

Kandefer-Szerszen M.

autor

Skalska-Kaminska A.

autor

Nowak-Kryska M.

autor

Niedziela P.

Bibliografia

• 1. LEE J.-Y., HWANG W.-I., LIM S.-T. Antioxidant and anticancer activities of organic extracts from Platycodon grandiflorum A. De Candolle roots. J. Ethnopharmacol., 93, 409, 2004.
• 2. ŠTĔRBOVÁ D., MATĔJIČEK D., VLČEK J., KUBÁŇ V. Combined microwave-assisted isolation and solid-phase purification procedures prior to the chromatographic determination of phanolic compounds in plant materials. Anal. Chim. Acta., 513, 435, 2004.
• 3. DORMAN H.J.D., PELTOKETO A., HILTUNEN R., TIKKANEN M.J. Characterization of the antioxidant properties of de-odourised aqueous extracts from selected Lamiaceae herbs. Food Chem., 83, 255, 2003.
• 4. JU E.M., LEE S.E., HWANG H.J., KIM J.H. Antioxidant and anticancer activity of extract from Betula platyphylla var. japonica. Life Sci., 74, 1013, 2004.
• 5. PADUCH R., WÓJCIAK-KOSIOR M., MATYSIK G. Investigation of biological activity of Lamii albi Flos extracts. J. Ethnopharmacol., 110, 69, 2007.
• 6. KOSAR M., DORMAN H.J.D., BACHMAYER O., BASER K.H.C., HILTUNEN R. An improved on-line HPLC-DPPH method for the screening of free radical scavenging compounds in water extracts of Lamiaceae plants. Chem. Nat. Comp., 39, 161, 2003.
• 7. CHUDNICKA A., MATYSIK G. Research of enzymatic activities of fresh juice and water infusions from dry herbs. J. Ethnopharmacol., 99, 281, 2005.
• 8. KOWALSKI R., WOLSKI T. TLC and HPLC analysis of the phenolic acids in Silphium perfoliatum L. leaves, inflorescences and rhizomes. J. Planar Chromatogr., 3, 230, 2003.
• 9. ŚWIĄTEK L., DOMBROWICZ E., ZADERNOWSKI R. Zawartość fenolokwasów w korzeniach goryczki żółtej (Gentiana lutea L.). Herba Pol., 34, 15, 1988 [In Polish].
• 10. JORK H., FUNK W., FISCHER W., WIMMER H.H. Thinlayer chromatography, reagents and detection methods, vol.1a, VCH Publisher, New York, 1990.
• 11. WAGNER H., BLADT S. Plant drug analysis. SpringerVerlag, Berlin, Heidelberg, New York, 1996.
• 12. GÜLÇIN İ. Antioxidant activity of caffeic acid (3,4-dihydroxycinnamic acid). Toxicology, 217, 213, 2006.
• 13. GÜLÇIN İ. Antioxidant and antiradical activities of L-carnitine. Life Sci., 78, 803, 2006.
• 14. FOTAKIS G., TIMBRELL J.A. In vitro cytotoxicity assays: Comparison of LDH, neutral red, MTT and protein assay in hepatoma cell lines following exposure to cadmium chloride. Toxicol. Lett., 160, 171, 2006.
• 15. HSU Y.-L., KUO P.-L., LIN Ch.-Ch. Proloferative inhibition, cell-cycle dysregulation, and induction of apoptosis by ursolic acid in human non-small cell lung cancer A549 cells. Life Sci., 75, 2303, 2004.
• 16. JAKUBOWICZ - GIL J., PADUCH R., PIERSIAK T., GŁOWNIAK K., GAWRON A., KANDEFER-SZERSZEŃ M. The effect of quercetin on pro-apoptotic activity of cisplatin in Hela cells. Biochem. Pharmacol., 69, 1343, 2005.
• 17. MA CH.-M., CAI S.-Q., CUI J.-R., WANG R.-Q., TU P.-F., HATTORI M., DANESHTALAB M. The cytotoxic activity of ursolic acid derivatives. Eur. J. Med. Chem., 40, 582, 2005.
• 18. TRIATHI R., MOHAN H., KAMAT J.P. Modulation of oxidative damage by natural products. Food Chem., 100, 81, 2007.
• 19. DI MAMBRO V.M., FONSECA M.J.V. Assays of physical stability and antioxidant activity of a topical formulation added with different plant extracts. J. Pharm. Biomed. Anal., 37, 287, 2005.
• 20. TROUILLAS P., CALLISTE C.-A., ALLAIS D.-P., SIMON A., MARFAK A., DELAGE CH., DUROUX J.-L. Antioxidant, anti-inflammatory and antiproliferative properties of sixteen water plant extracts used in the Limousin countryside as herbal teas. Food Chem., 80, 399, 2003.
• 21. KANG K.A., LEE K.W., ZHANG R., PIAO M., CHAE S., KIM K.N., JEON Y.J. PARK D.B., YOU H.J., KIM J.S. HYUN J.W. Caffeic acid protects hydrogen peroxide induced cell damage in WI-38 human lung fibroblast cells. Biol. Pharm. Bull., 29, 1820, 2006.
• 22. REBOLLEDO GODOY A.P.R., REBOLLEDO GODOY M.R., MEISSNER C., OEHMICHEN M. Proliferative activity of epidermal basal cells after wounding. AgNOR counts compared with bromodeoxyuridine reactivity in rats. Exp. Toxicol. Pathol., 53, 65, 2001.
• 23. DERENZINI M. The AgNORs. Micron, 31, 117, 2000.
• 24. GIUFFRÈ G., CARUSO R.A., BARRESI G., TUCCARI G. Prognostic significance of standardized AgNOR analysis in early and advanced gastric carcinomas. Virchows Arch., 433, 261, 1998.
• 25. KOULOUKOUSSA M., ALEPOROU-MARINOU V., ANGELOPOULOU B., TROUGAKOS I.P., PANAGOPOULOU E., KITTAS CHR., MARINOS E. Phorbol myristate acetate induces changes on F-actin and vinculin content in immature rat Sertoli cells. Tissue Cell., 36, 149, 2004.
• 26. MARTIN M., SIMON-ASSMANN P., KEDINGER M., MARTIN M., MANGEAT P., REAL F.X., FABRE M. DCC regulates cell adhesion in human colon cancer derived HT-29 cells and associates with ezrin. Eur. J. Cell Biol., 85, 769, 2006.
• 27. PANCHATCHARAM M., MIRIYALA S., GAYATHRI V.S., SUGUNA L. Curcumin improves wound healing by modulating collagen and decreasing reactive oxygen species. Mol. Cell Biochem., 290, 87, 2006.
• 28. BOTH D.M., GOODTZOVA K., YAROSH D.B., BROWN D.A. Liposome-encapsulated ursolic acid increases ceramides and collagen in human skin cells. Arch. Dermatol. Res., 293, 569, 2002.