Genetic characterisation of the cjaAB operon of Campylobacter coli

• Autorzy: Wyszynska A , Pawlowski M. , Bujnicki J. , Pawelec D. , Van Putten J.P.M. , Brzuszkiewicz E. , Jagusztyn-Krynicka E.K.
• Języki publikacji: EN

Abstrakty

EN

We investigated the regulation of the cjaA and cjaB genes of Campylobacter coll. These genes are seemingly arranged into one operon but appear to encode functionally different proteins i.e. an extracytoplasmic solute receptor and a MHS - metabolite: H⁺ symporter transport protein. Analysis of various transcriptional cjaA and/or cjaB lacZ fusion constructs revealed that both genes are arranged in an operon. RACE analysis located the transcription start site of the cjaAB operon 46 bp upstream of the translation start point. β-galactosidase reporter assays yielded much higher activity for the cjaA than the cjaB gene fusion products. RT-PCR showed unequal amounts of mRNA, indicating differential post-transcriptional processing of cjaA and cjaB mRNA possibly related to the presence of inverted repeats in the intergenic region. Phylogenetic analysis grouped CjaB into a new MHS sub-family together with potential transporters with uncharacterised functions of Campylobacter and Helicobacter. Notably, no CjaB family members were identified in ε-Proteobacteria from different ecological niches, such as H. hepaticas and Wolinella succinogenes.

Słowa kluczowe

EN

cjaB gene; Campylobacter coli; Campylobacter jejuni; gene expression; phylogenetic analysis; cjaAB operon; bacteria; cjaA gene

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2006
• Tom: 55
• Numer: 2
• Opis fizyczny: p.85-94,fig.,ref.

Twórcy

autor

Wyszynska A

• Warsaw University, Miecznikowa 1, 02-096 Warsaw, Poland

autor

Pawlowski M.

autor

Bujnicki J.

autor

Pawelec D.

autor

Van Putten J.P.M.

autor

Brzuszkiewicz E.

autor

Jagusztyn-Krynicka E.K.

Bibliografia

• Altschul S.F., T.L. Madden, A.A. Schaffer, J. Zhang, Z. Zhang, W. Miller and D.J. Lipman. 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25: 3389-3402.
• Barrios H., B. Valderrama and E. Morett. 1999. Compilation and analysis of sigma(54)-dependent promoter sequences. Nucleic Acids Res. 27: 4305-4313.
• Boos W. and J.M. Lucht. 1994. Periplasmic binding protein dependent ABC transporters, p. 1175-1210. In: H.E. Umbarger (eds), Escherichia coli and Salmonella. Cellular and Molecular Biology. American Society for Microbiology, Washington, DC.
• Bult C.J., O. White, G.J. Olsen, L. Zhou, R.D. Fleischmann, G.G. Sutton, J.A. Blake, L.M. Fitz Gerald, R.A. Clayton, J.D. Gocayne, A.R. Kerlavage, B.A. Dougherty, J.F. Tomb, M.D. Adams, C.I. Reich, R. Overbeek, E.F. Kirkness, K.G. Weinstock, J.M. Merrick, A. Glodek, J.L. Scott, N.S. Geoghagen and J.C. Venter. 1996. Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii. Science 273: 1058-1073.
• Chan V.L., E.K. Hani, A. Joe, J. Lynett, D. Ng and M. Steele. 2000. The hippurate hydrolase gene and other unique genes of Campylobacter jejuni, p. 455-463. In: M.J. Blaser (eds), Campylobacter, 2nd edition. ASM Press, Washington, DC.
• Coker A.O., R.D. Isokpehi, B.N. Thomas, K.O. Amisu and C.L. Obi. 2002. Human campylobacteriosis in developing countries. Emerg. Infect. Dis. 8: 237-244.
• Culham D.E., B. Lasby, A.G. Marangoni, J.L. Milner, B.A. Steer, R.W. van Nues and J.M. Wood. 1993. Isolation and sequencing of Escherichia coli gene proP reveals unusual structural features of the osmoregulatory proline/ betaine transporter, ProP. J. Mol. Biol. 229: 268-276.
• Deckert G., P.V. Warren, T. Gaasterland, W.G. Young, A.L. Lenox, D.E. Graham, R. Overbeek, M.A. Snead, M. Keller, M. Aujay, R. Huber, R.A. Feldman, J.M. Short, G.J. Olsen and R.V. Swanson. 1998. The complete genome of the hyperthermophilic bacterium Aquifex aeolicus. Nature 392: 353-358.
• Grunberg-Manago M. 1999. Messenger RNA stability and its role in control of gene expression in bacteria and phages. Annu. Rev. Genet. 33: 193-227.
• Jin S., A. Joe, J. Lynett, E.K. Hani, P. Sherman and V.L. Chan. 2001. JlpA, a novel surface-exposed lipoprotein specific to Campylobacter jejuni, mediates adherence to host epithelial cells. Mol. Microbiol. 39: 1225-1236.
• Kumar S., K. Tamura, I.B. Jakobsen and M. Nei. 2001. MEGA2: molecular evolutionary genetics analysis software. Bioinformatics 17: 1244-1245.
• Meinersmann R.J. and T.M. Wassenaar. 2003. Patterns in the intergenic regions of Campylobacter jejuni. Genome Letters 2: 28-33.
• Miller J.H. 1972. Experiments in Molecular Genetics. New York: Cold Spring Harbor.
• Pao S.S., I.T. Paulsen and M.H. Saier, Jr. 1998. Major facilitator superfamily. Microbiol. Mol. Biol. Rev. 62: 1-34.
• Parkhill J., B.W. Wren, K. Mungall, J.M. Ketley, C. Churcher, D. Basham, T. Chillingworth, R.M. Davies, T. Feltwell, S. Holroyd, K. Jagels, A.V. Karlyshev, S. Moule, M.J. Pallen, C.W. Penn, M.A. Quail, M.A. Rajandream, K.M. Rutherford, A.H. van Vliet, S. Whitehead and B.C. Barrell. 2000. The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences. Nature 403: 665-668.
• Pawelec D., E. Rozynek, J. Popowski and E.K. Jagusztyn-Krynicka. 1997. Cloning and characterization of a Campylobacter jejuni 72Dz/92 gene encoding a 30 kDa immunopositive protein, component of the ABC transport system; expression of the gene in avirulent Salmonella typhimurium. FEMS Immunol. Med. Microbiol. 19: 137-150.
• Petersen L., T.S. Larsen, D.W. Ussery, S.L. On and A. Krogh. 2003. RpoD promoters in Campylobacter jejuni exhibit a strong periodic signal instead of a-35 box. J. Mol. Biol. 326: 1361-1372.
• Saier M.H., Jr., J.T. Beatty, A. Goffeau, K.T. Harley, W.H. Heijne, S.C. Huang, D.L. Jack, P.S. Jahn, K. Lew, J. Liu, S.S. Pao, I.T. Paulsen, T.T. Tseng and P.S. Virk. 1999. The major facilitator superfamily. J. Mol. Microbiol. Biotechnol. 1: 257-279.
• Sambrook J. and D.W. Russell. 2001. Molecular Cloning. A Laboratory Manual. New York: Cold Spring Harbor.
• Spratt B.G., P.J. Hedge, S. te Heesen, A. Edelman and J.K. Broome-Smith. 1986. Kanamycin-resistant vectors that are analogues of plasmids pUC8, pUC9, pEMBL8 and pEMBL9. Gene 41: 337-342.
• Thompson J.D., T.J. Gibson, F. Plewniak, F. Jeanmougin and D.G. Higgins. 1997. The CLUSTAL X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 25: 4876-4882.
• Tomb J.F., O. White, A.R. Kerlavage, R.A. Clayton, G.G. Sutton, R.D. Fleischmann, K.A. Ketchum, H.P. Klenk, S. Gill, B.A. Dougherty, K. Nelson, J. Quackenbush, L. Zhou, E.F. Kirkness, S. Peterson, B. Loftus, D. Richardson, R. Dodson, H.G. Khalak, A. Głodek, K. McKenney, L.M. Fitzegerald, N. Lee, M.D. Adams and J.C. Venter. 1997. The complete genome sequence of the gastric pathogen Helicobacter pylori. Nature 388: 539-547.
• van Vliet A JH., J.D. Rock, L.N. Madeleine and J.M. Ketley. 2000. The iron-responsive regulator Fur of Campylobacter jejuni is expressed from two separate promoters. FEMS Microbiol. Lett. 188: 115-118.
• Wosten M.M., M. Boeve, M.G. Koot, A.C. van Nuene and B.A. van der Zeijst. 1998. Identification of Campylobacter jejuni promoter sequences. J. Bacteriol. 180: 594-599.
• Wyszyńska A., R. Godlewska and E.K. Jagusztyn-Krynicka. 2003. Product of the cjaA (cj()982c) Campylobacter jejuni gene is bifunctional protein, p. 71. In: XIIth International Workshop on Campylobacter, Helicobacter and related microrganisms. Aarhus, Danmark.