Cell separation with horizontal cell electrophoresis under near-isopycnic conditions on a 'density cushion'

• Autorzy: Wilk A , Urbanska K. , Woolley D.E. , Korohoda W.
• Języki publikacji: EN

Abstrakty

EN

This report describes an improvement made to the horizontal cell electrophoresis methodology. It involves using two liquid layers differing in density to produce an interface described as a “density cushion”. The electrophoretic system that employed an anti-convective porous matrix to separate red blood cells (RBC) and charged dyes effectively was found to be unsuitable for some other mammalian cells. The “density cushion” method was found to be more versatile and applicable to studies on the separation of a variety of cell types. The experiments described show the differences between the electrophoretic mobilities of a human eosinophilic leukaemia cell line (Eol-1) and RBC, both with and without the modification of the cell surface properties.

Słowa kluczowe

EN

cell electrophoresis; cell separation; cell surface; horizontal cell electrophoresis; erythrocyte; mammalian cell; cell surface property; density cushion method; electrochemical property; measurement

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2008
• Tom: 13
• Numer: 3
• Strony: 366-374
• Opis fizyczny: p.366-374,fig.,ref.

Twórcy

autor

Wilk A

• Jagiellonian University, Gronostajowa 7, 30-378 Krakow, Poland

autor

Urbanska K.

autor

Woolley D.E.

autor

Korohoda W.

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