Antioxidative defense to lead stress in subcellular compartments of pea root cells

• Autorzy: Malecka A. , Jarmuszkiewicz W. , Tomaszewska B.
• Języki publikacji: EN

Abstrakty

EN

Lead, similar to other heavy metals and abiotic factors, causes many unfavorable changes at the subcellular and molecular levels in plant cells. An increased level of superoxide anion in Pisum sativum root cells treated with 1 mM Pb(NO3)2 evidenced oxidative stress conditions. We found increased activities of enzymatic components of the antioxidative system (catalase and superoxide dismutase) in the cytosol, mito- chondrial and peroxisomal fractions isolated from root cells of Pisum sativum grown in modified Hoagland medium in the presence of lead ions (0.5 or 1 mM). Two isoenzyme forms of superoxide dismutase (Cu,Zn-SOD and Mn-SOD) found in differ­ent subcellular compartments of pea roots were more active in Pb-treated plants than in control. Increased amount of alternative oxidase accompanied by an increased ac­tivity of this enzyme was found in mitochondria isolated from lead-treated roots. These results show that plants storing excessive amounts of lead in roots defend them­selves against the harmful oxidative stress caused by this heavy metal.

Słowa kluczowe

EN

superoxide dismutase; lead stress; plant cell; oxidative stress; root cell; pea; lead; alternative oxidase; stress condition; superoxide anion; abiotic factor; mitochondrion; catalase; antioxidative system; Pisum sativum; peroxisome; heavy metal

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2001
• Tom: 48
• Numer: 3
• Opis fizyczny: p.687-698,fig.

Twórcy

autor

Malecka A.

• Adam Mickiewicz University, A.Fredry 10, 61-701 Poznan, Poland

autor

Jarmuszkiewicz W.

autor

Tomaszewska B.

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