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Zearalenone (ZEA) is an undesirable substance in feed materials and feed of plant origin. It is an example of the micotoxin that causes disturbances in the functioning of the reproductive system. The wide range of plant compounds in pet food means that ZEA may frequently have a negative effect on pet reproduction. An assessment of the influence of ZEA on the granulosa and theca cells of the ovarian follicle in bitches in vitro was carried out. The co-culture of the ovarian follicles was incubated for 72 hours with the addition of 12.5 ng/ml and 25.0 ng/ml of ZEA. Numerous vacuoles in the cytoplasm of the granulosa cells were noted in the culture with the addition of 25.0 ng/ml of ZEA. Preliminary investigations suggest negative effect of ZEA on the granulosa cells in bitches in vitro.
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p.305-309,fig.,ref.
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- University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-718 Olsztyn, Poland
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autor
Bibliografia
- Gajcka M, Jakimiuk E, Polak M, Otrocka-Domagala I, Janowski T, Zwierzchowski W, Obremski K, Zielonka L, Apoznanski J, Gaj^cki M (2004) Zearalenone applied per os provides adverse effects in structure of chosen parts of bitch reproductive system. Pol J Vet Sci 7: 59-66.
- Hsueh AJ, Adashi EY, Jones PB, Welsh THJr (1984) Hormonal regulation of differentiation of cultured ovarian granulose cells. Endocr Rev 5: 76-127.
- Kotsuji F, Kamitani N, Goto K, Kominaga T (1990) Bovine theca and granulosa cell interactions modulate their growth, morphology and function. Biol Reprod 43: 726-732
- Minervini F, DelPAquila ME, Maritato F, Minoia P, Vis- contini A (2001) Toxic effects of the mycotoxin zearalenone and it derivatives on in vitro maturation of bovine oocytes and 17 beta-estradiol levels in mural granulose cells culture. Toxicol In Vitro 15: 489-495.
- Scientific Committee on Food, 22 Jun. 2000, European Commission, Opinion of Scientific Committee on Food on Fusarium toxin. Part 2: Zearalenone (ZEA).
- Shier WT, Shier AC, Xie W, Mirocha CJ (2001) Structure-activity relationships for human estrogenic activity in zearalenone mycotoxins. Toxicol 39: 1435-1438.
- Stomczynska M (2002) Dynamics of arrangement of receptors of steroid hormones in ovary. Post Biol Kom 29: 21-46.
- Stokiosowa S (1982) Tissue culture of gonadal cells. Acta Biol Acad Sci Hung 33: 367-379.
- Stokiosowa S (2002) Cellular models in vitro in the study of reproduction. Molecular basis of reproduction in humans and other mammals. SRC instruction for authors ed etc., Poznan, pp 95-112.
- Stokiosowa S, Bahr J, Gregoraszczuk E (1978) Some morphological and functional characteristics of cells of the porcine theca interna in tissue culture. Biol Reprod 19: 712-719.
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Bibliografia
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