Mitochondrial DNA differentiation in chamois [genus Rupicapra]: implications for taxonomy, conservation, and management

• Autorzy: Hammer S. , Nadlinger K. , Hartl G.B.
• Języki publikacji: EN

Abstrakty

EN

Restriction fragment length polymorphisms (RFLPs) of mitochondrial (mt) DNA were used for investigating genetic differentiation in chamois (genus Rupicapra). Digestion of the mtDNAs of 58 individuals from 6 populations with a battery of 16 six-base cutting (restriction endonucleases yielded a total of 67 restriction sites. Based on the presence and absence of these restriction sites a total of 8 haplotypes could be defined. Six of them served for assessing genetic diversity within and among 4 local populations of R. rupicapra rupicapra. Estimates of nucleotide divergence among those haplotypes ranged from 0.05% to 0.25%. One chamois from the High Tatra (subspecies R. r. tatrica) was examined and showed the standard haplotype found in R. r. rupicapra. MtDNA in chamois from Catalunya, belonging to R. pyrenaica pyrenaica, was polymorphic for two haplotypes not found in any population of R. rupicapra. Mean nucleotide divergence among haplotypes found in R. rupicapra and R. pyrenaica was 0.56% (SD = 0.16%). Based on this value, an estimated divergence time of about 280 000 years suggests that the mtDNA lineages of R. rupicapra and R. pyrenaica separated prior to the Riss glacial in the later Pleistocene.

Słowa kluczowe

EN

restriction site; mitochondrial DNA; conservation; haplotype; Pleistocene; DNA; Rupicapra; taxonomy; chamois; population genetics

• Wydawca: -
• Czasopismo: Acta Theriologica. Supplement
• Rocznik: 1995
• Numer: 03
• Opis fizyczny: p.145-155,fig.

Twórcy

autor

Hammer S.

• Veterinarmedizinischen Universitat Wien, Savoyenstrasse 1, A-1160 Vienna, Austria

autor

Nadlinger K.

autor

Hartl G.B.

Bibliografia

• Avise J. C. 1994. Molecular markers, natural history and evolution. Chapman and Hall, New York, London: 1-511.
• Avise J. C., Arnold J., Ball R. M., Bermingham E., Lamb T., Neigel J. E., Reeb C. A. and Saunders N. 1987. The mitochondrial DNA bridge between population genetics and systematics. Annual Review of Ecology and Systematics 18: 489-522.
• Bandelt H.-J. 1992. Generating median graphs from Boolean matrices. [In: Li-statistical analysis. Y. Dodge, ed]. North-Holland, Elsevier: 305-309.
• Camerano L. 1914. Ricerche intorno ai camosci (Parte la, IIa, Ilia). Memorie della Regia Accademia di Scienze di Torino (Classe Scienze Fisiche Matematiche Naturali) 64(4): 1-82, 64(12): 1-88, 65(14): 1-82.
• Cronin M. A. 1993. Mitochondrial DNA in wildlife taxonomy and conservation biology: cautionary notes. Wildlife Society Bulletin 21: 339-348.
• Dolan J. M. 1963. Beitrag zur systematischen Gliederung des Tribus Rupicaprini Simpson 1945. Zeitschrift für zoologische Systematik und Evolutionsforschung 1: 311-407.
• Felsenstein J. 1993. PHYLIP (Phylogeny Inference Package), version 3.5c. Department of Genetics, University of Washington, Seattle.
• Hartl G. B., Burger H., Willing R. and Suchentrunk F. 1990. On the biochemical systematics of the Caprini and the Rupicaprini. Biochemical Systematics and Ecology 18: 175-182.
• Hartl G. B., Meneguz P. G., Apollonio M., Marco-Sanchez I., Nadlinger K. and Suchentrunk F. 1992. Molecular systematics of ibex in western Europe. Proceedings of the International Congress on the genus Capra in Europe, 20- 22.10.1992, Ronda (in press).
• Hartl G. B., Nadlinger K., Apollonio M., Markov G., Klein F., Lang G., Findo S. and Markowski J. 1995. Extensive mitochondrial-DNA differentiation among European red deer (Cervus elaphus) populations: implications for conservation and management. Zeitschrift für Säugetierkunde 60: 41-52.
• Hartl G. B., Suchentrunk F., Nadlinger K. and Willing R. 1993. An integrative analysis of genetic differentiation in the brown hare Lepus europaeus based on morphology, allozymes, and mito­chondrial DNA. [In: Ecological genetics in mammals. G. B. Hartl and J. Markowski, eds]. Acta Theriologica 38, Suppl. 2: 33-57.
• Hartl G. B., Willing R. and Nadlinger K. 1994. Allozymes in mammalian population genetics and systematics: Indicative function of a marker system reconsidered. [In: Molecular ecology and evolution: Approaches and applications. B. Schierwater, B. Streit, G. P. Wagner and R. DeSalle, eds]. Birkhäuser, Basel, Experientia, Suppl. 69: 299-310.
• Knaus W. and Schröder W. 1983. Das Gamswild. Paul Parey, Hamburg und Berlin.
• Lovari S. 1987. Evolutionary aspects of the biology of chamois, Rupicapra spp. (Bovidae, Caprinae). [In: The biology and management of Capricornis and related mountain antelopes. H. Soma, ed]. Croom-Helm, London: 51-61.
• Masini F. and Lovari S. 1988. Systematics, phylogenetic relationships and dispersal of the chamois (.Rupicapra spp.). Quaternary Research 30: 339-349.
• Miller C. 1987. Populationsgenetische Untersuchungen an Alpengams. Unpublished Ph D thesis, Ludwig-Maximillians-Universität München: 1-277.
• Miller C. and Hartl G. B. 1986. Genetic variation in two alpine populations of chamois CRupicapra rupicapra L.) Zeitschrift für Säugetierkunde 51: 114-121.
• Miller C. and Hartl G. B. 1987. Genetische Variation bei Gemsen der Alpen (.Rupicapra rupicapra rupicapra L.). Zeitschrift für Jagdwissenschaft 33: 220-227.
• Miller G. S. 1912. Catalogue of the mammals of Western Europe (Europe exclusive of Russia). British Museum (Natural History) Publications, London: XVI+1-1019.
• Nascetti G., Lanfranchi P., Ratti P., Peracino V., Mattiucci S., Meneguz P. G., Rossi L. and Bullini L. 1987. Studi elettroforetici sulla variabilita e divergenza genetica di Capra ibex ibex e Capra aegagrus hircus delle Alpi. [In: Lo stambecco delle Alpi: Realta attuale e prospettive. T. Balbo, D. DeMeneghi, P. G. Meneguz and L. Rossi, eds]. Universita di Torino, Torino: 11-15.
• Nascetti G., Lovari S., Lanfranchi P., Berducou C., Mattiucci S., Rossi L. and Bullini L. 1985. Revision of Rupicapra genus III. Electrophoretic studies demonstrating species distinction of chamois populations of the Alps from those of the Apennines and Pyrenees. [In: Biology and management of mountain ungulates. S. Lovari, ed]. Croom-Helm, London: 57-62.
• Nei M. 1972. Genetic distance between populations. American Naturalist 106: 283-292.
• Nei M. 1978. Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89: 583-590.
• Nei M. 1987. Molecular evolutionary genetics. Columbia University Press, New York: 1-512.
• Nei M. and Li W.-H. 1979. Mathematical model for studying genetic variation in terms of restriction endonucleases. Procedings of the National Academy of Sciences, USA 76: 5269-5273.
• Pemberton J. M., King P. W., Lovari S. and Bauchau V. 1989. Genetic variation in the Alpine chamois, with special reference to the subspecies Rupicapra rupicapra cartusiana Couturier 1938. Zeitschrift für Säugetierkunde 54: 243-250.
• Randi E., Tosi G., Toso S., Lorenzini R. and Fusco G. 1990. Genetic variability and conservation problems in Alpine ibex, domestic and feral goat populations (genus Capra). Zeitschrift für Säugetierkunde 55: 413-420.
• Rubin A. 1992. Untersuchungen über einen Zusammenhang zwischen Alloenzym-Polymorphismen und Kupfergehalten von Leber und Niere bei Wildwiederkäuern. Unpublished Diploma Thesis, University of Vienna: 1-53.
• Stüwe M., Scribner K. T. and Alkon P. U. 1992. A comparison of genetic diversity in Nubian ibex (Capra ibex nubiana) and Alpine ibex (Capra i. ibex). Zeitschrift für Säugetierkunde 57: 120-123.
• Templeton A. R., Hemmer H., Mace G., Seal U., Shields W. M. and Woodruff D. S. 1986. Local adaptation, coadaptation, and population boundaries. Zoo Biology 5: 115-125.
• Tiedemann R. 1994. Taxonomische und populationsgenetische Untersuchungen an der mitochon­drialen DNA von Eiderenten (Somateria mollissima L. 1758) und Watvögeln der Familien Haematopodidae, Charadriidae und Scolopacidae. Unpublished Ph D Thesis, Christian-Alb rechts- -Universität zu Kiel: 1-173.
• Wilson A. C., Cann R. E., Carr S. M., George M., Gyllensten U. B., Helm-Bychowski K. M., Higuchi R. G., Palumbi S. R., Prager E. M., Sage R. D. and Stoneking M. 1985. Mitochondrial DNA and two perspectives on evolutionary genetics. Biological Journal of the Linnean Society 26: 375-400.