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The studies aimed to evaluate the effect of experimental Aeromonas hydrophila vaccine on the total number of leukocytes, the number of neutrophils and T-lymphocytes and the phagocytic activity of the neutrophils and monocytes of carp (Cyprinus carpio L). The vaccine was given intraperitoneally or in an 1-hour bath. The number of leukocytes and the phagocytic activity were examined several times during a 42-day period after immunisation. Examinations were conducted at 12°C and 23°C. A visible increase of the total number of leukocytes and T-lymphocytes (21 days after immunisation) and neutrophils (7 to 14 days after immunisation) was found at 12°C. The percentage of engulfing phagocytes and their activity were significantly higher than those in the controls during the whole experimental period. A marked decrease in the total number of leukocytes was seen as early as 14 days after immunisation at 23°C; moreover, the number of neutrophils and T-lymphocytes was significantly lower on day 21 post immunisation as compared to that in the controls. On the other hand, the phagocytic activity was visibly higher than that in control fish but differences were less marked than those at 12°C.
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p.169-178,fig.
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Bibliografia
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