Czynniki wplywajace na dojrzewanie oocytow psa w warunkach in vitro

• Autorzy: Wlodarczyk R , Bukowska D. , Jaskowski J.M.

Warianty tytułu

EN

Factors involved in the maturation of dog oocytes in vitro

• Języki publikacji: PL

Abstrakty

EN

Modern methods of supported reproduction find little application in relation to dogs. Although there is some progress in the production dogs embryos in vitro, results to date remain far from unsatisfactory. The main cause of failures is the low ability of dog oocytes to end the maturation process. The percentage of dog oocytes matured in vitro does not exceed 24% while in different animals species this percentage was, respectively, in goats, cattle and pigs 94%, 74.5% and 58%. There are a good deal of studies to make optimal composition of a maturation medium. Oocyte incubation in a medium with the addition of hCG resulted in a maturation of 31.7% oocytes, while in a medium without hCG this percentage was 23.3%. Estrogens are important in the process of meiosis resumption and oocyte maturation. The addition of these hormones to the medium caused a significant increase of the percentage of maturated oocytes from 2.9% to 14.7% respectively in control and experimental groups. In a few studies the influence of such supplements as synthetic oviductal fluid (SOF) and the epithelial cells of oviduct on maturation dogs oocytes was definite. It seems that these supplements had no significant influence on initiation of meiosis resumption connected with oocyte maturation. However a positive influence on the enlargement of the percentage of matured dog oocytes was evoked by a culture condensation and an addition of cummulus cells.

Słowa kluczowe

PL

pozywki hodowlane; psy; dojrzewanie in vitro; oocyty; dojrzewanie oocytow; komorki rozrodcze; wielkosc oocytu; rozrod zwierzat; fazy cyklu plciowego

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2007
• Tom: 63
• Numer: 04
• Strony: 389-393
• Opis fizyczny: s.389-393,rys.,fot.,tab.,bibliogr.

Twórcy

autor

Wlodarczyk R

• Uniwersytet Przyrodniczy w Poznaniu, ul.Wojska Polskiego 52, 60-625 Poznan

autor

Bukowska D.

autor

Jaskowski J.M.

Bibliografia

• 1. Albarracin J. L., Morato R., Izquierdo D., Mogas T.: Effects of roscovitine on the nuclear and cytoskeletal components ot calf oocytes and their subsequent development. Theriogenology 2005, 64, 1740-1755.
• 2. Choi Y. H., Carnevale E. M., Seider E. G., Squire E. L.: Effects of gonadotrophins on bovine oocytes maturation in TCM-199. Theriogenology 2001, 56, 661-670.
• 3. De Avila Rodrigues B., Rodrigues J. L.: Influence of reproductive status on in vitro oocyte maturation in dogs. Theriogenology 2003, 60, 59-66.
• 4. De los Reyes M., De Lange J., Miranda P., Palominos J., Barros C.: Effect of human chorionic gonadotrophin supplementation during different culture periods on in vitro maturation of canine oocytes. Theriogenology 2005, 64, 1-11.
• 5. Durinzi K. L., Saniga E. M., Lanzendorf S. E.: The relationship between size and maturation in vitro in the unstimulated human oocyte. Fertil. Steril. 1995, 63, 404-406.
• 6. England G. C. W., Verstegen J. P., Hewn D. A.: Pregnancy following in vitro fertilisation of canine oocytes. Vet. Rec. 2001, 148, 20-22.
• 7. Eppig J. J.: Oocyte control of ovarian follicular development and function in mammals. Reproduction 2001, 122, 829-838.
• 8. Eppig J. J., Schroeder A. C., O'Brien M. J.: Developmental capacity of mouse oocytes maturated in vitro: effects of gonadotrophic stimulation, follicular origin and oocyte size. J. Reprod. Fertil. 1992, 95, 119-127.
• 9. Fuji M., Otoi T., Muramaki M., Tanaka M., Une S., Suzuki T.: The quality and maturation of bitch oocytes recovered from ovaries by the slicing method. J. Vet. Med. Sci. 1999, 62, 305-307.
• 10. Gilchrist R. B., Nayudu P. L., Nowshari M. A., Hodges J. K.: Meiotic competence of marmoset monkey oocytes is related to follicle size and oocytesomatic cell association. Biol. Reprod. 1995, 52, 1234-1243.
• 11. Hewitt D. A., England G. C.: Influence of gonadotrophin supplementation on the in vitro maturation of bitch oocytes. Vet. Rec. 1999, 144, 237-239.
• 12. Hewitt D. A., England G. C.: Synthetic oviductal fluid and oviductal cells coculture for canine oocyte maturation in vitro. Anim. Reprod. Sci. 1999, 55, 63-75.
• 13. Hewitt D. A., England G. C.: The effect ot oocyte size and bitch age upon oocyte nuclear maturation in vitro. Theriogenology 1997, 49, 957-966.
• 14. Kalab P., Srsen V., Farstad W., Krogenases A., Motlik J., Hafne A. L.: MAP kinase activation and RAF-1 synthesis in blue fox oocytes is controlled by cumulus granulose sells. Theriogenology 1997, 47, 400 (abstract).
• 15. Kim M. K., Fibrianto Y. H., Oh H. J., Jang G., Kim H. J., Lee K. S., Kang K. S., Lee B. C., Hwang W. S.: Effect of estradiol-17b and progesterone supplementation on in vitro nuclear maturation of canine oocytes. Theriogenology 2005, 63, 1342-1353.
• 16. Kito S., Bavister B. D.: Male pronuclear formation and early embryonic development of hamster oocytes maturated in vitro with gonadotrophins, amino acids and cysteamine. J. Reprod. Fertil. 1997, 110, 35-46.
• 17. Luvoni G. C.: Current progress on assisted reproduction in dog and cats: in vitro embryo production. Reprod. Nutr. Dev. 2000, 40, 505-512.
• 18. Luvoni G. C., Luciano A. M., Modina S., Gandolfi F.: Influence of different stages of estrus cycle of cumulus- oocyte communication in canine oocyte: effects on the efficiency of in vitro maturation. J. Reprod. Fertil. Suppl. 2001, 57, 141-146.
• 19. Mattioli M., Bacci M. L., Galeati G., Seren E.: Effect of LH and FSH on the maturation of pig oocytes in vitro. Theriogenology 1999, 36, 95-105.
• 20. Otoi T., Murakami M., Fujii M., Tanaka M., OOka A., Une S., Suzuki T.: Development of canine oocytes maturated and fertilised in vitro. Vet. Rec. 2000, 146, 52-53.
• 21. Otoi T., Willingham L., Shin T., Kraemer D. C., Westhusin M.: Effects of oocyte culture density on meiotic competence of canine oocytes. Reproduction 2002, 124, 775-781.
• 22. Parrott J. A., Skinner M. K.: Kit- ligand/stem cell factor induces primordial follicle development and initiates folliculogenesis. Endocrinology 1999, 140, 4262-4271.
• 23. Rodrigues B. A., Dos Santos L. C., Rodrigues J. L.: Embryonic development of in vitro matured and in vitro fertilized dog oocytes. Mol. Reprod. Dev. 2004, 67, 215-223.
• 24. Saint-Dizier M., Renard J. P., Chastant-Maillard S.: Induction of final maturation by sperm penetration in canine oocytes. Reproduction 2001, 121, 97-105.
• 25. Schoevers E. J., Kidson A., Verheijden J. H. M., Bevers M. M.: Effect of follicle-stimulating hormone on nuclear cytoplasmic maturation of sow oocytes in vitro. Theriogenology 2003, 59, 2017-2028.
• 26. Srsen V., Kalous J., Nagyova E., Sutovsky P., King W. A., Motlik J.: Effects of follicular stimulating hormone, bovine somatotrophin and okadaic acid on cumulus expansion and nuclear maturation of blue fox (Alopex lagopus) oocyte in vitro. Zygote 1998, 6, 299-309.
• 27. Tajik P., Shams Esfandabadi N.: In vitro maturation of caprine oocytes in different culture media. Small Ruminant Res. 2003, 47, 155-158.
• 28. Willingham-Rocky L. A., Hinrichs K., Westhusin M. E., Kraemer D. C.: Effects of stage of oestrous cycle and progesterone supplementation during culture on maturation of canine oocytes in vitro. Reproduction 2003, 126, 501-508.
• 29. Yamada S., Shimazu Y., Kawano Y., Nakazawa M., Naito K., Toyada Y.: Maturation, fertilization and development of oocytes in vitro. Biol. Reprod. 1992, 46, 853-858.
• 30. Zuelke K. A., Brackett B. G.: Luteinizing hormone enhanced in vitro maturation of bovine oocytes with or without protein supplementation. Biol. Reprod. 1990, 43, 784-787.