Carvedilol and adrenergic agonists suppress the lipopolysaccharide-induced NO production in RAW 264.7 macrophages via the adrenergic receptors

• Autorzy: Pekarova M. , Kralova J. , Kubala L. , Ciz M. , Papezikova I. , Macickova T. , Pecivova J. , Nosal R. , Lojek A.
• Języki publikacji: EN

Abstrakty

EN

The interaction of adrenergic agonists and/or antagonists with the adrenergic receptors expressed on immunologically active cells including macrophages plays an important role in regulation of inflammatory responses. Our study investigated the effects of carvedilol, a unique vasodilating b-adrenergic antagonist, and endogenous adrenergic agonists (adrenalin, noradrenalin, and dopamine) and/or antagonists (prazosin, atenolol) on lipopolysaccharide-stimulated nitric oxide (NO) production from murine macrophage cell line RAW 264.7. The production of NO was determined as the concentration of nitrites in cell supernatants (Griess reaction) and inducible nitric oxide synthase (iNOS) protein expression (Western blot analysis). Scavenging properties against NO were measured electrochemically. Carvedilol in a concentration range of 1, 5, 10 and 25 µM inhibited iNOS protein expression and decreased the nitrite concentration in cell supernatants. Adrenalin, noradrenalin or dopamine also inhibited the iNOS protein expression and the nitrite accumulation. Prazosine and atenolol prevented the effect of both carvedilol and adrenergic agonists on nitrite accumulation and iNOS expression in lipopolysaccharide-stimulated cells. These results, together with the absence of scavenging properties of carvedilol against NO, imply that both carvedilol and adrenergic agonists suppress the lipopolysaccharide-evoked NO production by macrophages through the activation and modulation of signaling pathways connected with adrenergic receptors.

Słowa kluczowe

EN

macrophage; adrenergic agonist; inflammatory response; nitric oxide; carvedilol; adrenergic receptor; lipopolysaccharide

• Wydawca: -
• Czasopismo: Journal of Physiology and Pharmacology
• Rocznik: 2009
• Tom: 60
• Numer: 1
• Strony: 143-150
• Opis fizyczny: p.143-150,fig.,ref.

Twórcy

autor

Pekarova M.

• Academy of Sciences of the Czech Republic, Kralovopolska 135, 612 65 Brno, Czech Republic

autor

Kralova J.

autor

Kubala L.

autor

Ciz M.

autor

Papezikova I.

autor

Macickova T.

autor

Pecivova J.

autor

Nosal R.

autor

Lojek A.