Warianty tytułu
Języki publikacji
Abstrakty
SNP-minisequencing has become common in forensic genetics, especially for analysing degraded or low copy number DNA (LCN DNA). The aim of this study was to examine the usefulness of five SNP (single nucleotide polymorphism) markers for analyzing degraded and LCN DNA recovered from archival samples. DNA extractions of eight formalin-fixed paraffin-embedded (FFPE) tissues were performed and DNA fragments were amplified in one multiplex PCR (polymerase chain reaction). SNPs were identified in a minisequencing reaction and a gel electrophoresis in ABI Prism®377 Sequencer. The research confirmed the usefulness of SNP-minisequencing for analysing FFPE tissues.
Wydawca
Czasopismo
Rocznik
Tom
Numer
Strony
815-819
Opis fizyczny
p.815-819,fig.,ref.
Twórcy
autor
- Medical University of Lodz, Sedziowska 18a, 91-304 Lodz, Poland
autor
Bibliografia
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Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
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