The influence of mercury and cadmium on the activities of some enzymes from Siberian sturgeon [Acipenser baeri Brandt 1869] semen

• Autorzy: Sarosiek B , Zdanio G. , Kowalski R.K. , Glogowski J.

Warianty tytułu

PL

Wplyw jonow rteci i kadmu na aktywnosc wybranych enzymow w nasieniu jesiotra syberyjskiego [Acipenser baeri Brandt 1869]

• Języki publikacji: EN

Abstrakty

EN

The effects of mercury and cadmium ions, at the concentrations of 100, 10 and 1 mg · dm⁻³, on the activity of enzymes contained in the sperm of Siberian sturgeon were determined. Whole semen was frozen without a cryoprotector. Acid phosphatase (AcP) activity decreased significantly following the application of mercury and cadmium ions at the highest concentration. Lactic dehydrogenase (LDH) was found to be the most sensitive to metal ions of all enzymes examined in the study. The concentration of 10 mg · dm⁻³ almost completely inhibited the activity of this enzyme. It was also found that mercury and cadmium ions slightly stimulated the activity of arylsulfatase (AS). The activity of β -N-acetylglucosaminidase (β -NAG) decreased considerably when mercury ions were added to the reaction mixture, in contrast to cadmium ions. The effects of Hg and Cd ions on the affinity of AcP and AS for substrates were also determined. It was found that these metal ions decreased the affinity of the above enzymes for p-nitrophenylphosphate and p-nitrocatechol sulfate respectively.

PL

W pracy określono wpływ jonów rtęci i kadmu o stężeniach 100, 10 i 1 mg · dm⁻³ na aktywność enzymów obecnych w nasieniu jesiotra syberyjskiego. Pełne nasienie zamrożono bez dodatku krioprotektora. Aktywność fosfatazy kwaśnej (AcP) statystycznie istotnie malała jedynie przy najwyższym zastosowanym stężeniu jonów rtęci i kadmu. Bardziej wrażliwa na obecność metali ciężkich okazała się dehydrogenaza mleczanowa (LDH). Rtęć o stężeniu 10 mg · dm⁻³ powodowała prawie całkowite zahamowanie aktywności tego enzymu. Wykazano także, że wymienione metale powodują nieznaczne (statystycznie nieistotne) podwyższenie aktywności arylosulfatazy (AS). Jony rtęci znacząco obniżały aktywność β-N-acetyloglukozoaminidazy ( β -NAG), natomiast jony kadmu nie wykazywały hamującego wpływu na aktywność tego enzymu. Określono również wpływ jonów rtęci i kadmu na powinowactwo fosfatazy kwaśnej i arylosulfatazy do substratu. Stwierdzono, że jony metali ciężkich zmniejszają powinowactwo wymienionych enzymów odpowiednio do p-nitrofenylofosforanu i p-nitrokatecholu.

• Wydawca: -
• Czasopismo: Polish Journal of Natural Sciences
• Rocznik: 2007
• Tom: 22
• Numer: 1
• Strony: 137-149
• Opis fizyczny: p.137-149,fig.,ref.

Twórcy

autor

Sarosiek B

• Polish Academy of Sciences, Bydgoska 1-8, 10-243 Olsztyn, Poland

autor

Zdanio G.

autor

Kowalski R.K.

autor

Glogowski J.

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