Aldolase A is present in smooth muscle cell nuclei

• Autorzy: Mamczur P. , Dzugaj A.
• Języki publikacji: EN

Abstrakty

EN

Previously we have shown that aldolase (ALD; EC 4.1.2.13) is present in cardiomyocyte nuclei. Now, we focused our attention on ALD localization in smooth muscle cells. Immunocytochemical methods were used to study the subcellular localization of ALD. Aldolase was localized in the cytoplasm as well as in the nuclei. Within the nuclei ALD was located in the heterochromatin region. Native polyacrylamide gel electrophoresis followed by aldolase activity staining in gel was used to study the ALD isoenzyme pattern in porcine smooth muscle cells. Two ALD isoenzymes, A and C, were found in these cells but in the nuclei only the muscle isoenzyme was detected. To support the nuclear localization of ALD, measurement of aldolase activity in the smooth muscle cell nuclei isolated from porcine stomach was performed. The ALD activity in the isolated nuclei was detectable only after preincubation of the nuclear fraction with Triton X-100 and high concentration of KCl.

Słowa kluczowe

PL

aldolase; heterochromatin; isoenzyme; nucleus; smooth muscle cell; localization; immunocytochemical method; cytoplasm

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2008
• Tom: 55
• Numer: 4
• Strony: 799-805
• Opis fizyczny: p.799-805,fig.,ref.

Twórcy

autor

Mamczur P.

• University of Wroclaw, Przybyszewskiego 65/77, 51-148 Wroclaw, Poland

autor

Dzugaj A.

Bibliografia

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