Faster and cheaper PCR on a standard thermocycler

• Autorzy: Sobczak K. , Kozlowski P. , Krzyzosiak W.J.
• Języki publikacji: EN

Abstrakty

EN

The PCR conditions have been optimized to make the process faster and more economical. When short DNA fragments are to be amplified, the time of denaturation, annealing and extension steps can be as short as 1 s each, and the yield of PCR product is still high, sufficient for many types of analysis. The PCR can be done even in a reaction volume as low as 1 jxl. The recommended volume, 2.5 jil or 5 jil, allows significant savings in the laboratory budget especially for laboratories which use PCR frequently and on a large scale.

Słowa kluczowe

EN

SSCP method; polymerase chain reaction

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1995
• Tom: 42
• Numer: 3
• Opis fizyczny: p.363-366,fig.

Twórcy

autor

Sobczak K.

• Polish Academy of Sciences, Z.Noskowskiego 12-14, 61-704 Poznan, Poland

autor

Kozlowski P.

autor

Krzyzosiak W.J.

Bibliografia

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