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Coculture of xylose-fermenting yeast P. stipitis CCY 39501 and respiratory deficient mutant of S. cerevisiae V30 designated as V30 I 40 was used for ethanol fermentation on a medium containing glucose and xylose mixture and compared to P. stipitis monoculture or coculture of P. stipitis and S. cerevisiae V30. Batch fermentations were carried out on a model medium or on a medium containing both sugars derived from direct saccharification of either wheat straw or birch sawdust. The yields obtained were 0.38 g/g, 0.34 g/g and 0.4 g/g for model medium, wheat straw and birch sawdust hydrolysates respectively, after cofermentation of P. stipitis with RD mutant V30 I 40. The results confirmed the application of this coculture for ethanol fermentation of sugars derived from lignocellulosic hydrolysates.
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http://www.ejpau.media.pl
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- Agricultural University of Lublin, 8 Skromna, 20-750 Lublin, Poland
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Bibliografia
- Awafo V.A., Chahal D.S., Simpson B.K., 1998, Optimization of ethanol production by Saccharomyces cerevisiae ATCC 60868 and Pichia stipitis Y-7124: a responce surface model for simultaneous hydrolysis and fermentation of wheat straw. J. Food Biochem.22: 489-509.
- Delgenes J.P., Moletta R., Preziosi-Belloy L., Navarro J.M., 1995, Alcoholic cofermentation of lignocellulosic sugars using a membrane recycle bioreactor. In: Proc. of the 8th European Biomass Conf., 2. Pergamon, Oxford: 1350-1355.
- Delgenes J.P., Laplace J.M., Moletta R., Navarro J.M., 1996, Comparative study of separated fermentations and cofermentation process to produce ethanol from hardwood derived hydrolysates. Biomass Bioeng. 11: 353-360.
- Grootjen R.D.J., Jansen M.L., van der Lans R.G.J.M., Luyben K.Ch.A.M., 1991, Reactors in series for the complete conversion of glucose/xylose mixtures by Pichia stipitis and Saccharomyces cerevisiae. Enzyme Microb. Technol. 13: 828-833.
- Hinfray C., Jouenne T., Junter G.A., 1995, Simultaneous fermentation of glucose and xylose by Saccharomyces cerevisiae and Candida shehatae coimmobilized in a double-chambered bioreactor. In: Proceedings of the 8th European Biomass Conference, vol 2. Pergamon, Oxford, 1291-1302.
- Ishihara M., Uemura S., Hayashi N., Shimizu K., 1991, Semicontinuous enzymatic hydrolysis of lignocelluloses. Biotechnol. Bioeng. 37: 948-954.
- Jeffries T.W., Kurtzman C.P., 1994, Strain selection, taxonomy, and genetics of xylose-fermenting yeasts. Enzyme Microb. Technol. 16: 922-932.
- Kellermann E., 1960, Quick method for determination of alcohol in solutions and mashes. Kvas. Prum. 1: 11-12 [in Czech].
- Kordowska-Wiater M., 2000, Ph.D. Thesis, Maria Curie Skłodowska University, Lublin, Poland.
- Kordowska-Wiater M., Targoński Z., 1997, Using of respiratory deficient mutants of Saccharomyces cerevisiae to glucose and xylose cofermentation with Pichia stipitis. Biotechnologia 4: 94-102 [in Polish].
- Laplace J.M., Delgenes J.P., Moletta R., 1992, Alcoholic glucose and xylose fermentations by the coculture process: compatibility and typing of associated strains. Can. J. Microbiol. 38: 654-658.
- Laplace J.M., Delgenes J.P., Moletta R., Navarro J.M., 1991, Alcoholic fermentation of glucose and xylose by Pichia stipitis, Candida shehatae, Saccharomyces cerevisiae and Zymomonas mobilis: oxygen requirement as a key factor. Appl. Microbiol. Biotechnol. 36: 158-162.
- Laplace J.M., Delgenes J.P., Moletta R., Navarro J.M., 1991, Combined alcoholic fermentation of D-xylose and D-glucose by four selected microbial strains: process considerations in relation to ethanol tolerance. Biotechnol. Lett. 13: 445-450.
- Laplace J.M., Delgenes J.P., Moletta R., Navarro J.M., [1993] Cofermentation of glucose and xylose to ethanol by a respiratory-deficient mutant of Saccharomyces cerevisiae co-cultivated with a xylose-fermenting yeast. J. Ferment. Bioeng. 75: 207-212.
- Laplace J.M., Delgenes J.P., Moletta R., Navarro J.M., 1993, Effects of culture conditions on the co-fermentation of glucose and xylose mixture to ethanol by a mutant of Saccharomyces diastaticus associated with Pichia stipitis. Appl. Microbiol. Biotechnol. 39: 760-763.
- Laplace J.M., Delgenes J.P., Moletta R., Navarro J.M., 1993c, Ethanol production from glucose and xylose by separated and coculture process using high cell density systems. Process Biochem. 28: 519-525.
- Moritz J.W., Duff S.J.B., 1996, Simultaneous saccharification and extractive fermentation of cellulosic substrates. Biotechnol. Bioeng. 49: 504-511.
- Picataggio S.K., Zhang M., Finkelstein M., 1994, Development of genetically engineered microorganisms for ethanol production. In: Enzymatic Conversion of biomass for fuels production. Am. Chem. Soc., Washington: 343-362.
- Preez du J.C., Bosch M., Prior B.A., 1987, Temperature profiles of growth and ethanol tolerance of the xylose-fermenting yeasts Candida shehatae and Pichia stipitis. Appl. Microbiol. Biotechnol. 25: 521-525.
- Preez du J.C., Prior B.A., 1985, A quantitative screening of some xylose-fermenting yeast isolates. Biotechnol. Lett. 7: 241-246.
- Szczodrak J., Fiedurek J., 1996, Technology for conversion of lignocellulosic biomass to ethanol. Biomass Bioeng. 10: 367-375.
- Szczodrak J., Targoński Z., [1989], Simultaneous saccharification and fermentation of cellulose: effect of ethanol and cellulases on particular stages. Acta Biotechnol. 9: 555-564.
- Taniguchi M., Tohma T., Itaya T., Fujii M., 1997, Ethanol production from a mixture of glucose and xylose by co-culture of Pichia stipitis and a respiratory-deficient mutant of Saccharomyces cerevisiae. J. Ferment. Bioeng. 83: 364-370.
- Zayed G., Meyer O., 1996, The single-batch bioconversion of wheat straw to ethanol emploing the fungus Trichiderma viride and the yeast Pachysolen tannophylus. Appl. Microbiol. Biotechnol. 45: 551-555.
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Bibliografia
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