YOR129c, a new element interacting with Cnm67p, a component of the spindle pole body of Saccharomyces cerevisiae

• Autorzy: Wysocka M. , Bialkowska A. , Micialkiewicz A. , Kurlandzka A.
• Języki publikacji: EN

Abstrakty

EN

The Saccharomyces cerevisiae spindle pole body (SPB) consists of numerous pro­teins forming the outer, inner and central plaques. The protein Cnm67 is an important component of the outer plaque. The C-terminus of this protein contains a determinant important for its SPB localization. We identified a protein encoded by YOR129c which interacts with this C-terminus in the two-hybrid system. YOR129c and CNM67 exhibit weak genetic interaction. The double deletion strain yor129cΔ cnm67Δ exhibits mod­erately increased resistance to 0.1 M LiCl and hygromycin B compared with the cnm67Δ single mutant. We propose that the YOR129c protein is an accessory factor associated with the cytoplasmic face of SPB and plays a role in cation homeostasis and/or multidrug resistance.

Słowa kluczowe

EN

hygromycin B; lithium tolerance; yeast; spindle pole body; new element; Saccharomyces cerevisiae

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2003
• Tom: 50
• Numer: 3
• Opis fizyczny: p.883-890,fig.

Twórcy

autor

Wysocka M.

• Polish Academy of Sciences, A.Pawinskiego 5A, 02-106 Warsaw, Poland

autor

Bialkowska A.

autor

Micialkiewicz A.

autor

Kurlandzka A.

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