Agrobacterium-mediated transformation of Bangladeshi Indica rices

• Autorzy: Al-Forkan M , Power J.B. , Anthony P. , Lowe K.C. , Davey M.R.
• Języki publikacji: EN

Abstrakty

EN

Morphologically normal, fertile transgenic plants were obtained by co-culturing embryogenic calli of the Bangladeshi Indica rice cultivars BR26 and Binni with Agrobacterium tumefaciens strain LBA4404 carrying the super binary vector pTOK233. Acetosyringone (100 μM) in the medium during coculture (25-28°C) and selection on hygromycin B (50 mg 1-1) were essential for efficient transformation. Stable integration and expression of β-glucuronidase, neomycin phosphotransferase and hygromycin phosphotransferase genes in regenerated plants were confirmed by histochemical and fluorometric assays, ELISA and Southern analysis. Two to 3 copies of T-DNA were integrated into regenerated plants; transgene expression did not correlate with gene copy number. Mendelian segregation of transgenes occurred in T1 seed progeny.

Słowa kluczowe

EN

transgenic Indica rice; transgene integration; Agrobacterium tumefaciens; transgenic plant; Oryza sativa; transgene expression; Bangladeshi Indica rice cultivar; rice

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2004
• Tom: 09
• Numer: 2
• Opis fizyczny: p.287-300,fig.,ref.

Twórcy

autor

Al-Forkan M

• University of Nottingham, University Park, Nottingham NG7 2RD, U.K.

autor

Power J.B.

autor

Anthony P.

autor

Lowe K.C.

autor

Davey M.R.

Bibliografia

• 1. Kinoshita, T. and Mori, K. In vitro techniques for genomic alteration in rice plants. Euphytica 120 (2001) 367-372.
• 2. Vasil, I.K. Cellular and molecular genetic improvement of cereals, in: Current Issues in Plant Molecular Biology (Terzi, M., Cella, R. and Falavigna, A. Eds.) Kluwer Academic Publishers, Dordrecht, The Netherlands, 1995, 1-8.
• 3. Tyagi, A.K., Mohanty, A., Bajaj, S., Chaudhury, A. and Maheshwari, S.C. Transgenic rice: A valuable monocot system for crop improvement and gene research. Critical Rev. Biotechnol. 19 (1999) 41-79.
• 4. Taylor, N.J. and Fauquet, C.M. Microparticle bombardment as a tool in plant science and agricultural biotechnology. DNA Cell Biol. 21 (2002) 963-977.
• 5. Dong, J.J., Kharb, P., Teng, W.M. and Hall, T.C. Characterization of rice transformed via an Agrobacterium-mediated inflorescence approach. Mol. Breed. 7 (2001) 187-194.
• 6. Hiei, Y., Komari, T. and Kubo, T. Transformation of rice by Agrobacterium tumefaciens. Plant Mol. Biol. 35 (1997) 205-218.
• 7. Tsugawa, H., Otsuki, Y. and Suzuki, M. Efficient transformation of rice protoplasts mediated by a synthetic polycationic amino polymer. Theor. Appl. Genet. 97 (1998) 1019-1026.
• 8. Vain, P., James, V.A., Worland, B. and Snape, J.W. Transgene behaviour across two generations in a large random population of transgenic rice plants produced by particle bombardment. Theor. Appl. Genet. 105 (2002) 878-889.
• 9. Linsmaier, E.M. and Skoog, F. Organic growth factor requirements for tobacco tissue cultures. Physiol. Plant. 18 (1965) 100-127.
• 10. Hiei, Y., Ohta, S., Komari, T. and Kumashiro, T. Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA. The Plant J. 6 (1994) 271-282.
• 11. Azhakanandam, K., McCabe, M.S., Power, J.B., Lowe, K.C., Cocking, E.C. and Davey, M.R. T-DNA transfer, integration, expression and inheritance in rice: effects of plant genotype and Agrobacterium super-virulence. J. Plant Physiol. 157 (2000) 429-439.
• 12. Murashige, T. and Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15 (1962) 437-497.
• 13. Azhakanandam, K., Lowe, K.C., Power, J.B. and Davey, M.R. Haemoglobin (Erythrogen)-enhanced mitotic division and plant regeneration from cultured protoplasts (Oryza sativa L.). Enzyme Microb. Technol. 21 (1997) 572- 577.
• 14. Jefferson, R.A., Kavanagh, T.A. and Bevan, M.W. GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J. 6 (1987) 3901-3907.
• 15. Bradford, M.M. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72 (1976) 248-254.
• 16. Dellaporta, S.L., Wood, J. and Hicks, J.B. A plant DNA mini preparation: version II. Plant Mol. Biol. Rep. 1 (1983) 19-21.
• 17. McCabe, M.S., Mohapatra, U.B., Debnath, S.C., Power, J.B. and Davey, M.R. Integration, expression and inheritance of two linked T-DNA marker genes in transgenic lettuce. Mol. Breed. 5 (1999) 329-344.
• 18. Sambrook, J., Fritsch, E.F. and Maniatis, T. Molecular Cloning: A Laboratory Manual, 2nd Edn., 1989, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
• 19. McCabe, M.S., Power, J.B., de Laat A.M.M. and Davey, M.R. Detection of single-copy genes in DNA from transgenic plants by nonradioactive Southern blot analysis. Mol. Biotechnol. 7 (1997) 1-6.
• 20. Andras, S.C., Hartman, T.P.V., Marshall, J.A., Marchant, R., Power, J.B., Cocking, E.C. and Davey, M.R. A drop-spreading technique to produce cytoplasm-free mitotic preparations from plants with small chromosomes. Chromosome Res. 7 (1999) 641-647.
• 21. Snedecor, G.W. and Cochran, W.G. Statistical Methods, 8th Edn., 1989, Iowa State University Press, Ames.
• 22. Khanna, H.K. and Raina, S.K. Agrobacterium-mediated transformation of indica rice cultivars using binary and superbinary vectors. Aust. J. Plant Physiol. 26 (1999) 311-324.
• 23. Mohanty, A, Sarma, N.P. and Tyagi, A.K. Agrobacterium-mediated high frequency transformation of an elite indica rice variety Pusa Basmati 1 and transmission of the transgenes to R2 progeny. Plant Sci. 147 (1999) 127-137.
• 24. Sheng, J. and Citovsky, V. Agrobacterium-plant cell DNA transport: have virulence proteins will travel. The Plant Cell 8 (1996) 1699-1710.
• 25. Dillen, W., de Clercq, J., Kapila, J., Zambre, M., van Montagu, M. and Angenon, G. The effect of temperature on Agrobacterium tumefaciens-mediated gene transfer to plants. The Plant J. 12 (1997) 1459-1463.
• 26. Hood, E.E., Helmer, G.L., Fraley, R.T. and Chilton, M-D. The hypervirulence of Agrobacterium tumefaciens A281 is encoded in a region of pTiBo542 outside of
• T-DNA. J. Bacteriol. 168 (1986) 1291-1301.
• 27. Jin, S., Komari, T., Gordon, M.P. and Nester, E.W. Genes responsible for the super-virulence phenotype of Agrobacterium tumefaciens A281. J. Bacteriol. 169 (1997) 4417-4425.
• 28. Davey, M.R., Ingram, H., Azhakanandam, K. and Power, J.B. The genetic transformation of rice and maize, in: Cereal Biotechnology. (Morris, P.C. and Bryce, J.H. Eds.) Woodhead Publishing Ltd., Cambridge, 1999, 43-69.
• 29. Curtis, I.S., Power, J.B., Blackhall, N.W., de Laat, A.M.M. and Davey, M.R. Genotype-independent transformation of lettuce using Agrobacterium tumefaciens. J. Exp. Bot. 45 (1994) 1441-1449.
• 30. Chan, M-T., Chang, H-H., Ho, S-L., Tong, W-F. and Yu, S-M. Agrobacterium-mediated production of transgenic rice plants expressing a chimeric α-amylase promoter/β-glucuronidase gene. Plant Mol. Biol. 22 (1993) 491-506.
• 31. Aldemita, R.R. and Hodges, T.K. Agrobacterium tumefaciens-mediated transformation of Japonica and Indica rice varieties. Planta 199 (1996) 612-617.
• 32. Uzé, P., Wunn, J., Puonti-Kaerlas, J., Potrykus, I. and Sautter, C. Plasmolysis of precultured immature embryos improves Agrobacterium-mediated gene transfer to rice (Oryza sativa L.). Plant Sci. 130 (1997) 87- 95.
• 33. Li, H-Q., Sautter C., Potrykus, I. and Puonti-Kaerlas, J. Genetic transformation of cassava (Manihot esculenta Crantz). Nature Biotechnol. 14 (1996) 736-740.