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Shiga-like toxin-producing (SLTEC) Escherichia coli strains are one of the most important food borne emerging pathogens. One hundred and fifty-seven E. coli strains isolated from 39 children with diarrhea of unknown origin and one hundred and five E. coli strains from 20 healthy children were examined for Shiga-like toxin production in Vero cell line assay. The synthesis of Shiga-like toxin was observed on Vero cell line and confirmed by PCR for one of 262 E. coli strains tested. The shiga-like toxin II-positive E. coli strain was isolated from 2-years old healthy child with no symptoms of gastrointestinal tract infection.
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p.125-126,fig.,ref.
Twórcy
autor
- University of Medicine, 4 Chalubinskiego Street, 50-368 Wroclaw, Poland
autor
autor
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Bibliografia
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- Karch H., M. Bielaszewska, M. Bitzan and H. Schmidt. 1999. Epidemiology and diagnosis of shiga toxin-producing Escherichia coli infections. Diagn. Microbiol. Infect. Dis. 34: 229-243.
- Konowalchuk J., J.I. Speirs and S. Stavric. 1977. Vero response to a cytotoxin of Escherichia coli. Infect. Immun. 18: 775-779.
- Lindquist R. 1997. Preparation of PCR samples from food by a rapid and simple centrifugation technique evaluated by detection of Escherichia coli 0157:H7. Int. J. Food. Microbiol. 37: 73-78.
- Morabito S., H. Karch, P. Mariani-Kurkdijan, H. Schmidt, F. Mine Hi, E. Bingen and A. Caprioli. 1998. Enteroaggregative, shiga toxin producing Escherichia coli 0111:H2 associated with an outbreak of hemolytic-uremic syndrome. J. Clin. Microbiol. 36: 840-842.
- Ritchie M., S. Partington, J. Jessop and M.T. Kelly. 1992. Comparison of a direct fecal shiga-like toxin assay and sorbitol - MacConkey agar culture for laboratory diagnosis of enterohemorrhagic Escherichia coli infection. J. Clin. Microbiol. 30: 461-464.
- Weaver J.W. and M.T. Rowe. 1997. Effect of non-targed cells on the sensitivity of the PCR for Escherichia coli 0157:H7. Lett. Applied. Microbiol. 25: 109-112.
- Wieler L.H., B. Busse, H. Steinruck, L. Beutin, L. Weber, H. Karch and G. Baljer. 2000. Enterohemorrhagic Escherichia coli (EHEC) strains of serogroup 0118 display three distinctive clonal groups of EHEC pathogens. J. Clin. Microbiol. 38: 2162-2169.
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Bibliografia
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