Effects of aflatoxin B1, ochratoxin A, patulin, citrinin, and zearalenone on the in vitro proliferation of pig blood lymphocytes

• Autorzy: Stec J , Zmudzki J. , Rachubik J. , Szczotka M.
• Języki publikacji: EN

Abstrakty

EN

The effects of aflatoxin B₁ (AFB₁), ochratoxin A (OTA), patulin (PAT), citrinin (CIT), and zearalenone (ZEA) on in vitro response of pig peripheral blood mononuclear cells to mitogen concanavalin A was assayed after three days of incubation using ³H- thymidine uptake. Dose response curves for each mycotoxin were generated and the concentrations producing 50% inhibition of cell proliferation (IC₅₀) were estimated. AFB₁, OTA, and PAT were the most potent toxins with the IC₅₀ of 0.06, 0.17, and 0.19 µmol/L, respectively (0.2, 0.7, and 0.3 µg/mL, respectively). Based on the molar concentration, the inhibition potencies relative to that of AFB₁ were determined. OTA had 35% and PAT 31% potency to that of AFB₁, but CIT and ZEA had only 1.6 and 1.9 of AFB₁ inhibition potencies.

Słowa kluczowe

EN

zearalenone; citrinin; proliferation assay; patulin; mycotoxin; lymphocyte; pig; aflatoxin B1; blood lymphocyte; feed contamination; ochratoxin

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2009
• Tom: 53
• Numer: 1
• Strony: 129-134
• Opis fizyczny: p.129-134,fig.,ref.

Twórcy

autor

Stec J

• National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Zmudzki J.

autor

Rachubik J.

autor

Szczotka M.

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