The effect of lead on photosynthesis and respiration in detached leaves and in mesophyll protoplasts of Pisum sativum

• Autorzy: Parys E , Romanowska E. , Siedlecka M. , Poskuta J.W.
• Języki publikacji: EN

Abstrakty

EN

Photosynthesis and transpiration rate of detached leaves of pea (Pisum sativum L. cv. Iłowiecki) exposed to solution of Pb(NO₃)₂ at 1 or 5 mmol·dm⁻³ concentrations were inhibited. The higher concentration of this toxicant decreased photosynthesis and transpiration rates 2 and 3 times respectively, and increased respiration by about 20 %, as measured after 24 hours of treatment. Similarly to Pb(NO₃)₂, glyceraldehyde solution, an inhibitor of phosphoribulokinase, at 50 mmol·dm⁻³ concentration decreased the rates of photosynthesis and transpiration during introduction into pea leaves. The rate of dark respiration, however, remained unchanged during 2 hours of experiment. The potential photochemical efficiency of PS II (Fv/Fm) and the activity of Rubisco (EC 4.1.1.39) at 5 mmol·dm⁻³ of Pb(NO₃)₂ were lowered by 10 % and 20 % respectively, after 24 hours. Neither changes in the activity of PEPC (EC 4.1.1.31) or protein and pigment contents were noted in Pb-treated leaves. The photosynthetic activity of protoplasts isolated from leaves treated for 24 or 48 hours with Pb(NO₃)₂ at 5 mmol·dm⁻³ concentration was decreased 10 % or 25 %, whereas, the rate of dark respiration was stimulated by about 40 % and 75 %, respectively. The content of abscisic acid, a hormone responsible for stomatal closure, in detached pea leaves treated for 24 h with 5 mmol·dm⁻³ of Pb(NO₃)₂ solution was increased by about 3 times; a longer (48h) treatment led to further increase (by about 7 times) in the amount of this hormone. The results of our experiments provide evidences that CO₂ fixation in detached pea leaves, at least up to 24 hours of Pb(NO₃)₂ treatment, was restricted mainly by stomatal closure.

Słowa kluczowe

EN

pigment; protoplast; fluorescence; abscisic acid; transpiration; lead; leaf; glyceraldehyde; Pisum sativum; respiration; photosynthesis

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 1998
• Tom: 20
• Numer: 3
• Opis fizyczny: p.313-322,fig.

Twórcy

autor

Parys E

• University of Warsaw, 00-927 Warsaw, Krakowskie Przedmiescie 26-28, Poland

autor

Romanowska E.

autor

Siedlecka M.

autor

Poskuta J.W.

Bibliografia

• Arteca R. N., Tsai D. 1987. Effects of abscisic acid on the photosynthesis, transpiration and growth of tomato plants. Crop. Res. (Hort. Res) 27: 91–96.
• Barceló J., Poschenrieder Ch. 1990. Plant water relations as affected by heavy metal stress: A review. J. Plant Nutr. 13: 1–37.
• Barceló J., Cabot C., Poschenrieder Ch. 1986. Cadmium-induced decrease of water stress resistance in bush bean plants (Phaseolus vulgaris L. cv. Contender). II. Effects of Cd on endogenous abscisic acid levels. J. Plant Physiol. 125: 27–34.
• Bazzaz F. A., Rolfe G. L., Carlson R. W. 1974. Effect of Cd on photosynthesis and transpiration of excised leaves of corn and sunflower. Physiol. Plant. 32: 373–376.
• Bazzaz F. A., Carlson R. W., Rolfe G. L. 1975. Inhibition of corn and sunflower photosynthesis by lead. Physiol. Plant. 34: 326–329.
• Becerril J. M., González-Murua C., Muńoz-Rueda A., De Felipe M. R. 1989. Changes induced by cadmium and lead in gas exchange and water relations of clover and lucerne. Plant Physiol. Biochem. 27: 913–918.
• Bittell J. F., Koeppe D. E., Miller R. J. 1974. Sorption of heavy metal cations by corn mitochondria and the effects on electron and energy transfer reactions. Physiol. Plant. 30: 226–230.
• Clijsters H., van Assche F. 1985. Inhibition of photosynthesis by heavy metals. Photosynthesis Res. 7: 31–40.
• Foy C. D., Chaney R. L., White M. C. 1978. The physiology of metal toxicity in plants. Pl. Physiol. 29: 511–566.
• Greger M., Ögren E. 1991. Direct and indirect effects of Cd²⁺ on photosynthesis in sugar beat (Beta vulgaris). Physiol. Plant. 83: 129–135.
• Hurry V., Tobiaeson M., Krömer S., Gardeström P., Öquist G. 1995. Mitochondria contribute to increased photosynthetic capacity of leaves of winter rye (Secale cereale L.) following cold-hardening. Plant Cell Environ. 18: 69–76.
• Koeppe D. E., Miller R. J. 1970. Lead effects on corn mitochondrial respiration. Science 167: 1376–1377.
• Krömer S. 1997. Respiration during photosynthesis. Annu. Rev. Plant Physiol. Plant Mol. Biol. 46: 45–70
• Krömer S., Sitt M., Heldt H. 1988. Mitochondria oxidative phosphorylation participating in photosynthetic metabolism of a leaf cell. FEBS Letters 226: 352–356.
• Krömer S., Heldt H. W. 1991. Respiration of pea leaf mitochondria and redox transfer between the mitochondrial and extramitochondrial compartment. Biochem. Biophys. Acta 1057: 42–50.
• Krömer S., Malmberg G., Gardeström P. 1993. Mitochondrial contribution to photosynthetic metabolism. A study with barley (Hordeum vulgare L.) leaf protoplasts at different light intensities and CO₂ concentrations. Plant Physiol. 102: 947–955.
• Krupa Z., Öquist G., Huner N. P. A. 1992. The influence of cadmium an primary photosystem II photochemistry in bean as revealed by chlorophyll a fluorescence — a preliminary study. Acta Physiol. Plant. 14: 71–76.
• Krupa Z., Öquist G., Huner N. P. A. 1993. The effects of cadmium on photosynthesis of Phaseolus vulgaris — a fluorescence analysis. Physiol. Plant. 88: 626–630.
• Lamoreaux R. J., Chaney W. R. 1978. The effect of cadmium on net photosynthesis, transpiration, and dark respiration of excised silver maple leaves. Physiol. Plant. 43: 231–236.
• Lee K. C., Cunningham B. A., Paulsen G. H., Liang G. M., Moore R. B. 1976. Effects of cadmium on respiration rate and activities of several enzymes in soybean seedlings. Physiol. Plant. 36: 4–6.
• Lichtenthaler H. K., Wellburn R. R. 1983. Determination of total carotenoids and chlorophylls a and b on leaf extracts in different solvents. Biochem. Soc. Trans. 603: 591–592.
• Loveys B. R., van Dijk H. M. 1988. Improved extraction of abscisic acid from plant tissue. Aust. J. Plant Physiol. 15: 421–427.
• Malla Reddy M., Vani T., Raghavendra A. S. 1991. Light-enhanced dark respiration in mesophyll protoplasts from leaves of pea. Plant Physiol. 96: 1368–1371.
• Mertens R., Deus-Neumann B., Weiler E. W. 1983. Monoclonal antibodies for the detection and quantitation of the endogenous plant growth regulator, abscisic acid. FEBS Letters 160: 269–272.
• Miller R. J., Bittel J. E., Koeppe D. E. 1973. The effect of cadmium on electron and energy transfer reactions in corn mitochondria. Physiol. Plant. 28: 166–171.
• Öquist G., Wass R. 1988. A portable, microprocessor operated instrument for measuring chlorophyll fluorescence kinetics in stress physiology. Physiol. Plant. 73: 211–217.
• Parys E., Romanowska E., Lechowicz W., Poskuta J. 1989. Postillumination burst of CO₂ in maize and reed grass leaves determined in a closed gas exchange system. Plant Physiol. Biochem. 27: 27–34.
• Poschenrieder C., Gunse B., Barceló J. 1989. Influence of cadmium on water relations, stomatal resistance and abscisic acid content in expanding bean leaves. Plant Physiol. 90: 1365–1371.
• Poskuta J. W., Parys E., Romanowska E. 1987. The effects of lead on the gaseous exchange and photosynthetic carbon metabolism of pea seedlings. Acta Soc. Bot. Pol. 56: 127–137.
• Poskuta J. W., Parys E., Romanowska E. 1996. Toxicity of lead to photosynthesis, accumulation of chlorophyll, respiration and growth of Chlorella pyrenoidosa. Protective role of dark respiration. Acta Pysiol. Plant. 18: 165–171.
• Poskuta J. W., Wacławczyk-Lach E. 1995. In vivo responses of primary photochemistry of photosystem II and CO₂ exchange in light and in darkness of tall fescue genotypes to lead toxicity. Acta Physiol. Plant. 17: 233–240.
• Romanowska E., Treumann H-J. 1992. Effect of red light on ribulose −1.5-bisphosphate carboxylase activity in pea leaves. Biologia Plantarum 33: 505–509.
• Slabas A. R., Walker D. A. 1976. Inhibition of spinach phosphoribulokinase by DL-glyceraldehyde. Biochem. J. 153: 613–619.
• Stokes D. M., Walker D. A. 1972. Photosynthesis by isolated chloroplasts. Inhibition by DL-glyceraldehyde of carbon dioxide assimilation. Biochem. J. 128: 1147–1157.
• Tardieu F., Davies W. J. 1992. Stomatal response to abscisic acid is a function of current plant water status. Plant Physiol. 98: 540–545.
• Vani T., Malla Reddy M., Ragavendra A. S. 1990. Beneficial interactions between photosynthesis and respiration in mesophyll protoplasts of pea during short-dark cycles. Physiol. Plant. 80: 467–471.
• Walker D. 1987. The use of the oxygen electrode and fluorescence probes in simple measurements of photosynthesis. Research Institute for Photosynthesis, The University of Sheffield, Sheffield, S10 2TN. UK., pp.: 116–120. ISBN 1-870-23200-3.
• Weigel H. J. 1985a. Inhibition of photosynthetic reactions of isolated intact chloroplasts by cadmium. J. Plant Physiol. 119: 179–189.
• Weigel H. J. 1985b. The effect of Cd²⁺ on photosynthetic reactions of mesophyll protoplasts. Physiol. Plant. 63: 192–200.