Green tea effect on antioxidant status of erythrocytes and on haematological parameters in rats

• Autorzy: Zapora E , Holub M. , Waszkiewicz E. , Dabrowska M. , Skrzydlewska E.
• Języki publikacji: EN

Abstrakty

EN

The aim of this study was to estimate the influence of ethanol intoxication as well as in vitro effect of ethanol, acetaldehyde, and tBOOH on antioxidant status of erythrocytes and on haematological parameters in rats. It has been shown that ethanol intoxication caused a decrease in SOD, CAT, GSH-Px, and GSSG-R activities and in GSH and vitamin E levels in erythrocytes as well as in RBC count, and HGB and HCT values. These changes were accompanied by an enhanced lipid peroxidation estimated on the basis of MDA level. Green tea administration partially prevented the observed changes. In the in vitro experiment, ethanol and to a higher degree acetaldehyde and tBOOH caused a decrease in the activity of erythrocyte antioxidant enzymes (SOD, CAT, GSH-Px, GSSG-R) and in the concentration of non-enzymatic antioxidants (GSH, vitamins E and C). All the examined compounds enhanced lipid peroxidation process. However, a green tea due to its antioxidant properties partially protected erythrocytes against ethanol, acetaldehyde, and tBOOH action.

Słowa kluczowe

EN

antioxidant status; acetaldehyde; oxidative stress; human organism; hematological parameter; rat; ethanol; antioxidant; erythrocyte; green tea; animal organism

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2009
• Tom: 53
• Numer: 1
• Strony: 139-145
• Opis fizyczny: p.139-145,ref.

Twórcy

autor

Zapora E

• Medical University of Bialystok, 15-089 Bialystok, Poland

autor

Holub M.

autor

Waszkiewicz E.

autor

Dabrowska M.

autor

Skrzydlewska E.

Bibliografia

• 1. Aebi H.: Catalase in vitro. Meth Enzymol 1984, 105, 121-126.
• 2. Akkus I., Gultekin F., Akoz M., Caglayan O., Bahcaci S., Can U.G., Ay M., Gurel A.: Effect of moderate alcohol intake on lipid peroxidation in plasma, erythrocyte and leukocyte and on some antioxidant enzymes. Clin Chim Acta 1997, 266, 141-147.
• 3. Arora A., Byrem T.M., Nair M.G., Strasburg G.M.: Modulation of lyposomal membrane fluidity by flavonoids and isoflavonoids. Arch Biochem Biophys 2000, 373, 102-109.
• 4. Atamna H., Ginsburg H.: Heme degradation in the presence of glutathione. J Biol Chem 1995, 270, 24876- 24883.
• 5. Bengtsson G., Smith-Kielland A., Morland J.: Ethanol effect on protein synthesis in non-parenchymal liver cells, hepatocytes and density populations of hepatocytes. Exp Mol Pathol 1984, 41, 44-57.
• 6. Bilska A., Kryczyk A., Włodek L.: The different aspects of the biological role of glutatione. Postępy Hig Med Dosw 2007, 61, 438-453.
• 7. Braun K.P., Cody R.B., Jones D.R., Peterson C.M.: A structural assignment for a stable acetaldehyde-lysine adducts. J Biol Chem 1995, 270, 11263-11266.
• 8. Chen L., Yang X., Jiao H., Zhao B.: Tea catechins protect against lead induced cytotoxicity, lipid peroxidation, and membrane fluidity in HepG2 cells. Toxicol Sci 2002, 69, 149-156.
• 9. Chen S.T., Chen S.Y., Tu C.C., Chiou S.H., Wang K.T.: Physicochemical properties of alkaline serine proteases in alcohol. J Prot Chem 1995, 14, 205-215.
• 10. Cimen M.Y.: Free radical metabolism in human erythrocytes. Clin Chim Acta 2008, 390, 1-11.
• 11. Crespy V., Williamson G.: A review of the health effects of green tea catechins in in vivo animal models. J Nutr 2004, 134, 3431S-3440S.
• 12. Dean P.J.: Radical-free biology of oxidative stress. Am J Physiol 2008, 295, C849-C868.
• 13. De Leenheer A., De Bevere V., De Ruyter M.G., Claeys A.C.: Simultaneous determination of retinol and α- tocopherol in human serum by HPLC. J Chromatog 1979, 162, 408-413.
• 14. Dobrzyńska I., Szachowicz-Petelska B., Ostrowska J., Skrzydlewska E., Figaszewski Z.: Protective effect of green tea on erythrocyte membrane of different age rats intoxicated with ethanol. Chem Biol Interact 2005, 10, 41-53.
• 15. Frei B., Higdon J.V.: Antioxidant activity of tea polyphenols in vivo: evidence from animal studies. J Nutr 2003, 133, 3275-3284.
• 16. Gabbianelli R., Cifani C., Massi M., Polidori C., Falcioni G.: Oxidative damage in rat erythrocyte membranes following ethanol intake: effect of ethyl pyruvate. Chem Biol Interact 2007, 169, 122-131.
• 17. Genc S., Gurdol F., Oner-Iyidogan Y., Onaran I.: The effect of melatonin administration on ethanol-induced lipid peroxidation in rat. Pharmacol Res 1998, 37, 37-40.
• 18. Guo Q., Zhao B., Li M., Shen S., Xin W.: Studies on protective mechanism of four components of green tea polyphenols against lipid peroxidation in synaptosomes. Biochim Biophys Acta 1996, 1304, 210-222.
• 19. Henning S.M., Niu Y., Liu Y., Lee N.H., Hara Y., Thames G.D., Minutti R.R., Carpenter C.L., Wang H., Heber D.: Bioavailability and antioxidant effect of epigallocatechin gallate administered in purified form versus a green tea extract in healthy individuals. J Nutr Biochem 2005, 16, 610-616.
• 20. Londero D., Lo Greco P.L.: Automated high- performance liquid chromatographic separation with spectrofluorometric detection of a malondialdehyde- thiobarbituric acid adduct in plasma. J Chromatogr A 1996, 729, 207-210.
• 21. Łuczaj W., Waszkiewicz E., Skrzydlewska E., Roszkowska-Jakimiec W.: Green tea protection against age-dependent ethanol-induced oxidative stress. J Toxicol Environ Health A 2004, 67, 595-606.
• 22. Maiani G., Serafini M., Salicci M., Azzini E., Ferro- Luzzi A.: Application of a new high-performance liquid chromatographic method for measuring selected polyphenols in human plasma. J Chromatogr B 1997, 692, 311-317.
• 23. Mize C.E., Langdon R.G.: Hepatic glutathione reductase. I. Purification and general kinetic properties. J Biol Chem 1962, 237, 1589-1595.
• 24. Nicholls R.M., Fowles L.F., Worrall S., de Jersey J., Wilce P.A.: Distribution and turnover of acetaldehyde- modified proteins in liver and blood of ethanol-fed rats. Alcohol Alcohol 1994, 29, 149-157.
• 25. Ostrowska J., Łuczaj W., Kasacka I., Rózański A., Skrzydlewska E.: Green tea protects against ethanol- induced lipid peroxidation in rat organs. Alcohol 2004, 32, 25-32.
• 26. Paglia D.E., Valentine W.N.: Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 1967, 70, 158- 169.
• 27. Pryor W.A., Houk K.N., Foote C.S., Fukuto J.M., Ignarro L.J., Squadrito G.L., Davies K. J. A.: Free radical biology and medicine: it's a gas man! Am J Physiol Regulatory Intergrative Comp Physiol 2006, 291, R491-R511.
• 28. Saija A., Scalese M., Lanza M., Marzullo D., Bonina F., Castelli F.: Flavonoids as antioxidant agents: importance of their interaction with biomembranes. Free Radic Biol Med 1995, 19, 481-486.
• 29. Sykes J.A., McCormac F.X., O'Breien T.J.: Preliminary study of the superoxide dismutase content of some human tumors. Cancer Res 1978, 38, 2759-2762.
• 30. Tsuchiya H.: Effects of green tea catechins on membrane fluidity. Pharmacology 1999, 59, 34-44.
• 31. Wakimoto M., Masuoka N., Nakano T., Ubuka T.: Determination of glutathione peroxidase activity and its contribution to hydrogen peroxide removal in erythrocytes. Acta Med Okayama 1998, 52, 233-237.
• 32. Wickramasinghe S.N.: Role of superoxide anion radicals in ethanol metabolism by blood monocyte-derived human macrophages. J Exp Med 1989, 169, 755-763.
• 33. Zhao B., Guo Q., Xin W.: Free radical scavenging by green tea polyphenols. Methods Enzymol 2001, 335, 217-231.
• 34. Zima T., Fialová L., Mestek O., Janebová M., Crkovská J., Malbohan I., Stípek S., Mikulíková L., Popov P.: Oxidative stress, metabolism of ethanol and alcohol- related diseases. J Biomed Sci 2001, 8, 59-70.