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Abstrakty
We have estimated the number of 5S rRNA genes in Aspergillus nidulans using two-dimensional agarose gel electrophoresis and hybridization to appropriate probes, representing the 5'-halves, the 3'-halves of the 5S rRNA sequence and a sequence found at the 3'-end of all known A. nidulans pseudogenes (block C). We have found 23 5S rRNA genes, 15 pseudogenes consisting of the 5'-half of the 5S rRNA sequence (of which 3 are flanked by block C) and 12 copies of block C which do not seem to be in the vicinity of 5S rRNA sequences. This number of genes is much lower than our earlier estimates, and makes our previously analyzed sample of 9 sequenced genes and 3 pseudogenes much more representative.
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Opis fizyczny
p.449-452,fig.
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autor
- University of Warsaw, A.Pawinskiego 5a, 02-106 Warsaw, Poland
autor
autor
Bibliografia
- 1. Bartnik, E., Strugała, K. & Stępień, P.P. (1981) Goning and analysis of recombinant plasmids containing genes for Aspergillus nidulans 5S rRNA. Curr Genet. 4,173-176.
- 2. Bartnik, E., Bartoszewski, S., Borsuk, P. & Empel, J. (1986) Aspergillus nidulans 5S rRNA genes and pseudogenes. Curr. Genet. 10, 453-457.
- 3. Bartoszewski, S., Borsuk, P., Kem, I. & Bartnik, E. (1987) Microheterogeneity in Aspergillus nidulans 5S rRNA genes. Curr. Genet. 11, 571-573.
- 4. Borsuk, P., Gniadkowski, M., Bartnik, E. & Stępień, P.P. (1989) Unusual evolutionary conservation of 5S rRNA pseudogenes in Aspergillus nidulatis: similarity of the DNA sequence associated with the pseudogenes with the mouse immunoglobulin switch region. /. Mol. Evol. 28,125-130.
- 5. Gniadkowski, M., Fiett, J., Borsuk, P., Stępień, P.P. & Bartnik, E. (1990) Structure and evolution of 5S rRNA genes and pseudogenes in the genus Asj)ergillus. J. Mol. Evol. 33,175-178.
- 6. Yi, M., Au, L., Ichikawa, N. & Ts'o, P.O.P. (1990) Enhanced resolution of DNA restriction fragments: a procedure by two-dimensional electrophoresis and double-labeling. Proc. Natl. Acad. Sci. U.S.A. 87,3919-3923.
- 7. Van Ryk, D.I., Lee, Y. & Nazar, R.N. (1990) Efficient expression and utilization of mutant 5 S rRNA in Saccharwnyces cerevisiae. J. Biol. Chan. 265,8377-8381.
Typ dokumentu
Bibliografia
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bwmeta1.element.agro-article-6058eb23-56b4-45d8-9b6b-7d95a8ff7c91