The optimal eukaryotic signal for translation initiation from non-aug codons, present upstream of bacteriophage lambda P cistron, is inactive in Escherichia coli

• Autorzy: Wrobel B , Slominski B. , Wegrzyn G.
• Języki publikacji: EN

Abstrakty

EN

Expression of the replication genes of bacteriophage λ, O and P, is believed to be translationally coupled. However, it was previously noted that, under conditions of amino acid starvation, when O is not synthesized, P continues to be expressed at a relatively high level. The results presented in this report, contrary to the previously presented hypothesis, suggest that an AGACUGGAU sequence (an optimal context for translation initiation from non-AUG codons in eukaryotes, and present upstream the P cistron) is inactive in Escherichia coli. Comparative sequence analysis confirms that such a signal is unlikely to be important for P synthesis. Instead, a weak Shine-Dalgarno sequence may be present upstream the P cistron, and be active in the absence of O gene expression.

Słowa kluczowe

EN

bacteriophage; translation reinitiation; cell; S-gal [3,4-cyclohexenoesculetin-beta-D-galacto-pyranoside; replication gene; Escherichia coli; amino acid

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2003
• Tom: 08
• Numer: 2
• Opis fizyczny: p.305-310,fig.

Twórcy

autor

Wrobel B

• Polish Academy of Sciences, Sw.Wojciecha 5, 81-347 Gdynia, Poland

autor

Slominski B.

autor

Wegrzyn G.

Bibliografia

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