Cathepsins and BID are involved in the molecular switch between apoptosis and autophagy in breast cancer MCF-7 cells exposed to camptothecin

• Autorzy: Lamparska-Przybysz M. , Gajkowska B. , Motyl T.
• Języki publikacji: EN

Abstrakty

EN

The details of molecular switching points between apoptosis and autophagy in tumor cells have still not been fully elucidated. This study focused on the role of cathepsin B and its substrate, BID as molecular links between apoptosis and autophagy in human breast cancer MCF-7 cells exposed to camptothecin. Apoptosis occurred rapidly with a peak in 60 min after drug administration, whereas autophagy developed at a much slower rate with continuous progression during 24 h of cell exposure to the drug. CPT induced very rapid activation of cathepsin B. Inhibition of cathepsins by E64d prevented CPT-induced BAX and BID aggregation on mitochondria and reduced significantly reduced apoptotic cell number. The above effects were accompanied by an increase in autophagosome formation, measured by expression of MAP I LC3. BID knock down resulted in strong suppression of CPT-induced apoptosis and a shift of cell death towards autophagy, manifesting with an increase of Beclin 1 and MAP I LC3 cellular content.

Słowa kluczowe

EN

camptothecin; MCF-7 cell; breast cancer; apoptosis; Beclin 1; cathepsin; autophagy; tumour cell

• Wydawca: -
• Czasopismo: Journal of Physiology and Pharmacology. Supplement
• Rocznik: 2005
• Tom: 56
• Numer: 3
• Strony: 159-179
• Opis fizyczny: p.159-179,fig.,ref.

Twórcy

autor

Lamparska-Przybysz M.

• Warsaw Agricultural University, Nowoursynowska 159, 02-776 Warsaw, Poland

autor

Gajkowska B.

autor

Motyl T.