Expression analysis of Cucurbita cDNA encoding endonuclease

• Autorzy: Szopa J.
• Języki publikacji: EN

Abstrakty

EN

The nuclear matrices of plant cell nuclei display intrinsic nuclease activity which consists in nicking supercoiled DNA. A cDNA encoding a 32 kDa endonuclease has been cloned and sequenced. The nucleotide and deduced amino-acid sequences show high homology to known 14-3-3 protein sequences from other sources. The amino-acid sequence shows agreement with consensus sequences for potential phosphorylation by protein kinase A and C and for calcium, lipid and membrane-binding sites. The nucleotide-binding site is also present within the conserved part of the sequence. By Northern blot analysis, the differential expression of the corresponding mRNA was detected; it was the strongest in sink tissues. The endonuclease activity found on DNA-polyacrylamide gel electrophoresis coincided with mRNA content and was the highest in tuber.

Słowa kluczowe

EN

supercoiled DNA; protein kinase A; protein kinase C; endonuclease; tuber; blotting; calcium; plant endonuclease; mRNA; polacrylamide gel electrophoresis; nuclear matrix; cDNA; Cucurbita pepo

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1995
• Tom: 42
• Numer: 2
• Opis fizyczny: p.183-189,fig.

Twórcy

autor

Szopa J.

• Wroclaw University, S.Przybyszewskiego 63, 51-148 Wroclaw, Poland

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