Flavonoid transport across RBE4 cells: a blood-brain barrier model

• Autorzy: Faria A , Pestana D. , Teixeira D. , Azevedo J. , De Freitas V. , Mateus N. , Calhau C.
• Języki publikacji: EN

Abstrakty

EN

There is a growing interest in dietary therapeutic strategies to combat oxidative stress-induced damage to the Central Nervous System (CNS), which is associated with a number of pathophysiological processes, including Alzheimer’s and Parkinson’s diseases and cerebrovascular diseases. Identifying the mechanisms associated with phenolic neuroprotection has been delayed by the lack of information concerning the ability of these compounds to enter the CNS. The aim of this study was to evaluate the transmembrane transport of flavonoids across RBE-4 cells (an immortalized cell line of rat cerebral capillary endothelial cells) and the effect of ethanol on this transport. The detection and quantification of all of the phenolic compounds in the studied samples (basolateral media) was performed using a HPLC-DAD (Diode Array Detector). All of the tested flavonoids (catechin, quercetin and cyanidin-3-glucoside) passed across the RBE-4 cells in a time-dependent manner. This transport was not influenced by the presence of 0.1% ethanol. In conclusion, the tested flavonoids were capable of crossing this blood-brain barrier model.

Słowa kluczowe

EN

flavonoids; transport; rat; endothelial cell; blood-brain barrier; anthocyanin; flavonol

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2010
• Tom: 15
• Numer: 2
• Opis fizyczny: p.234-241,fig.,ref.

Twórcy

autor

Faria A

• University of Porto, Al.Prof.Hernani Monteiro, 4200-319 Porto, Portugal

autor

Pestana D.

autor

Teixeira D.

autor

Azevedo J.

autor

De Freitas V.

autor

Mateus N.

autor

Calhau C.

Bibliografia

• 1. Hollman, P.C. and Katan, M.B. Dietary flavonoids: intake, health effects and bioavailability. Food Chem. Toxicol. 37 (1999) 937-942.
• 2. Van Duyn, M.A. and Pivonka, E. Overview of the health benefits of fruit and vegetable consumption for the dietetics professional: selected literature. J. Am. Diet. Assoc. 100 (2000) 1511-1521.
• 3. Hu, J.P., Calomme, M., Lasure, A., De Bruyne, T., Pieters, L., Vlietinck, A. and Vanden Berghe, D.A. Structure-activity relationship of flavonoids with superoxide scavenging activity. Biol. Trace Elem. Res. 47 (1995) 327-331.
• 4. Rice-Evans, C.A., Miller, N.J. and Paganga, G. Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free Radic. Biol. Med. 20 (1996) 933-956.
• 5. Aquilano, K., Baldelli, S., Rotilio, G. and Ciriolo, M.R. Role of nitric oxide synthases in Parkinson's disease: a review on the antioxidant and anti-inflammatory activity of polyphenols. Neurochem. Res. 33 (2008) 2416- 2426.
• 6. Singh, M., Arseneault, M., Sanderson, T., Murthy, V. and Ramassamy, C. Challenges for research on polyphenols from foods in Alzheimer's disease: bioavailability, metabolism, and cellular and molecular mechanisms. J. Agric. Food Chem. 56 (2008) 4855-4873.
• 7. Hertog, M.G., Hollman, P.C., Katan, M.B. and Kromhout, D. Intake of potentially anticarcinogenic flavonoids and their determinants in adults in The Netherlands. Nutr. Cancer 20 (1993) 21-29.
• 8. Hertog, M.G.L., Hollman, P.C.H. and Katan, M.B. Content of potentially anticarcinogenic flavonoids of 28 vegetables and 9 fruits commonly consumed in The Netherlands. J. Agric. Food Chem. 40 (1992) 2379-2383.
• 9. Lee, M.J., Wang, Z.Y., Li, H., Chen, L., Sun, Y., Gobbo, S., Balentine, D.A. and Yang, C.S. Analysis of plasma and urinary tea polyphenols in human subjects. Cancer Epidemiol. Biomarkers Prev. 4 (1995) 393-399.
• 10. Yang, G.Y., Liu, Z., Seril, D.N., Liao, J., Ding, W., Kim, S., Bondoc, F. and Yang, C.S. Black tea constituents, theaflavins, inhibit 4-(methylnitrosamino) -1-(3-pyridyl)-1-butanone (NNK)-induced lung tumorigenesis in A/J mice. Carcinogenesis 18 (1997) 2361-2365.
• 11. Frankel, E.N., Waterhouse, A.L. and Teissedre, P.L. Principal phenolic phytochemicals in selected California wines and their antioxidant activity in inhibiting oxidation of human low-density lipoproteins J. Agric. Food Chem. 43 (1995) 890-894.
• 12. Arts, I.C.W., Hollman, P.C.H. and Kromhout, D. Chocolate as a source of tea flavonoids. Lancet 354 (1999) 488-488.
• 13. Oliveira, J., de Freitas, V., Silva, A.M.S. and Mateus, N. Reaction between hydroxycinnamic acids and anthocyanin-pyruvic acid adducts yielding new portisins. J. Agric. Food Chem. 55 (2007) 6349-6356.
• 14. Sousa, C., Mateus, N., Silva, A.M.S., Gonzalez-Paramas, A.M., SantosBuelga, C. and de Freitas, V. Structural and chromatic characterization of a new malvidin 3-glucoside-vanillyl-catechin pigment. Food Chem. 102 (2007) 1344-1351.
• 15. Yi, W., Akoh, C.C., Fischer, J. and Krewer, G. Absorption of anthocyanins from blueberry extracts by caco-2 human intestinal cell monolayers. J. Agric. Food Chem. 54 (2006) 5651-5658.
• 16. Lau, F.C., Shukitt-Hale, B. and Joseph, J.A. Age-related neuronal and behavioral deficits are improved by polyphenol-rich blueberry supplementation. In: Oxidative stress and age-related neurodegeneration 2006. pp. 373-393. Crc Press-Taylor & Francis Group, Boca Raton.
• 17. Davalos, A., Castilla, P., Gomez-Cordoves, C. and Bartolome, B. Quercetin is bioavailable from a single ingestion of grape juice. Int. J. Food Sci. Nutr. 57 (2006) 391-398.
• 18. Manach, C., Williamson, G., Morand, C., Scalbert, A. and Remesy, C. Bioavailability and bioefficacy of polyphenols in humans. I. Review of 97 bioavailability studies. Am. J. Clin. Nutr. 81 (2005) 230S-242S.
• 19. Mullen, W., Edwards, C.A. and Crozier, A. Absorption, excretion and metabolite profiling of methyl-, glucuronyl-, glucosyl- and sulphoconjugates of quercetin in human plasma and urine after ingestion of onions. Br. J. Nutr. 96 (2006) 107-116.
• 20. Mazza, G., Kay, C.D., Cottrell, T. and Holub, B.J. Absorption of anthocyanins from blueberries and serum antioxidant status in human subjects. J. Agric. Food Chem. 50 (2002) 7731-7737.
• 21. Youdim, K.A., Dobbie, M.S., Kuhnle, G., Proteggente, A.R., Abbott, N.J. and Rice-Evans, C. Interaction between flavonoids and the blood-brain barrier: in vitro studies. J. Neurochem. 85 (2003) 180-192.
• 22. Regina, A., Roux, F. and Revest, P.A. Glucose transport in immortalized rat brain capillary endothelial cells in vitro: transport activity and GLUT1 expression. Biochim. Biophys. Acta 1335 (1997) 135-143.
• 23. Faria, A., Pestana, D., Azevedo, J., Martel, F., de Freitas, V., Azevedo, I., Mateus, N. and Calhau, C. Absorption of anthocyanins through intestinal epithelial cells - Putative involvement of GLUT2. Mol. Nutr. Food Res. 53 (2009) 1-8.
• 24. Youdim, K.A., Hale, B.S. and Joseph, J.A. Flavonoids and the brain: interactions at the blood–brain barrier and their physiological effects on the central nervous system. Free Radic. Biol. Med. 37 (2004) 1683-1693.
• 25. Dragoni, S., Gee, J., Bennett, R., Valoti, M. and Sgaragli, G. Red wine alcohol promotes quercetin absorption and directs its metabolism towards isorhamnetin and tamarixetin in rat intestine in vitro. Br. J. Pharmacol. 147 (2006) 765-771.
• 26. Assuncao, M., Santos-Marques, M.J., de Freitas, V., Carvalho, F., Andrade, J.P., Lukoyanov, N.V. and Paula-Barbosa, M.M. Red wine antioxidants protect hippocampal neurons against ethanol-induced damage: a biochemical, morphological and behavioral study. Neuroscience 146 (2007) 1581-1592.
• 27. Papandreou, M.A., Dimakopoulou, A., Linardaki, Z.I., Cordopatis, P., Klimis-Zacas, D., Margarity, M. and Lamari, F.N. Effect of a polyphenolrich wild blueberry extract on cognitive performance of mice, brain antioxidant markers and acetylcholinesterase activity. Behav. Brain Res. 198 (2009) 352-358.