The influence of methisoprinol applied in ovo upon hatchability and health status of turkeys

• Autorzy: Tykalowski B. , Stenzel T. , Mazur-Lech B. , Andrzejewski M. , Koncicki A.
• Języki publikacji: EN

Abstrakty

EN

A study was undertaken to determine the effect of a synthetic immunomodulator, i.e. methisop- rinol applied in ovo, upon the hatchability of turkey poults under conditions of a standard hatchery as well as on their health status evaluated based on analyses of selected biochemical indices in their blood serum. Experiments were conducted on 5 groups of BUT 9 turkeys at the age of 5 days (35 birds in each group) hatched from eggs to which methisoprinol (VetAgro, Lublin, Poland) was applied in ovo at a dose of 5 mg (group I), 10 mg (group II) or 20 mg per egg (group III) on the 26th day of incubation. Turkeys hatched from eggs to which a physiological solution of NaCl was applied on the same day at a dose of 0.1 ml per egg (group IV) as well as those hatched from eggs without in ovo injection (group V) served as controls. Five hundreds eggs were used in each group. Hatchability was evaluated based on the number of hatched poults in respect of the number of eggs with live embryos transferred from the setting compartment to the hatching compartment, that were subjected to in ovo administration of the preparations according to the experimental design. Blood serum of the 5-day-old turkey poults was analyzed for activities of AST, ALP, LDH-L, CK, lysozyme and cerulop- lasmine as well as for total protein and albumin contents. Analyses were also conducted for the immune system organ index - percentage contribution of organs of the immune system (spleen, thymus and the bursa of Fabricius) in the body weight of turkeys. The study demonstrated that methisoprinol administered to turkey embryos in ovo on day 26 of incubation at doses of 5, 10 or 20 mg per embryo did not induce any disturbances in the hatching process or affect its final result. In addition, it was shown not to exert any negative effect on the health status of the reared turkey poults.

Słowa kluczowe

EN

turkey; immunomodulation; methisoprinol; in ovo application; health status; hatchability; blood serum; biochemical index; embryo; immune system; bird

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2009
• Tom: 12
• Numer: 2
• Strony: 203-207
• Opis fizyczny: p.203-207,ref.

Twórcy

autor

Tykalowski B.

• University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-719 Olsztyn, Poland

autor

Stenzel T.

autor

Mazur-Lech B.

autor

Andrzejewski M.

autor

Koncicki A.

Bibliografia

• Borzemska W, Karpinska E, Kosowska G, Szeleszczuk P, Binek M, Malicka E, Malec H, Niedziôlka J (1996) Stu­dies on Inosine pranobex embryotoxicity in biological test on chicken embryos. Med Weter 52: 778-780.
• Dietert RR, Lamont SJ (1994) Avian immunology: from fundamental immune mechanism to the integrative man­agement of poultry. Poult Sci 73: 975-978.
• Dietert RD, Golemboski KA (1994) Environment-immune interactions. Poult Sci 73 : 1062-1076.
• Dohms JE, Saif YM (1997) Criteria for evaluation im- munosupression. Avian Dis 28: 305-310.
• Douglas J, Buddiger N (2002) How today’s social, political and consumer - driven environment influences the busi­ness objectives of the primary breeders: genotype, envi- ronment and nutrition interactions. 4th Internat Sympo­sium on Turkey Diseases, Berlin, pp. 1-12.
• Friedman A, Bartov I, Sklan D (1998) Humoral immune response impairment following excess vitamin E nutrition in the chick and turkey. Poult Sci 77: 956-962.
• Grondel JL, Angenent GC, Egberts E (1985) The influence of antibiotics on the immune system. Investigations on the cellular functions of chicken leukocytes in vitro. Vet Immunol Immunopathol 10: 307-16.
• Guiro S, Koncicki A (2004) Vaccination against NDV in turkeys infected by haemorrhagic enteritis vims. Med Weter 60: 871-873.
• Haygreen EA, Kaiser P, Burgess SC, Davison TF (2006) In ovo DNA immunisation followed by recombinant fowl pox boost is fully protective to challenge with virulent IBDV. Vaccine 24: 4951-4961.
• Johnston PA, Liu H, O’Connell T, Phelps P, Bland M, Ty- czkowski J, Kemper A, Harding T, Avakian A, Haddad E, Whitfill C, Gildersleeve R, Ricks CA (1997) Applica­tions of in ovo technology. Poultry Sci 76:165-178.
• Koncicki A, Rrasnodęska-Depta A, Zduńczyk Z, Jankowski J, Wróblewska M, Falkowska A (2000) Biochemical indi­ces in blood and tissues of turkeys fed feed mixtures con­taining fat of different oxidation degree. Pol J Vet Sci 3: 81-86.
• Koncicki A, Rumińska E, Stenzel T, Mazur-Gonkowska B, Tykalowski B (2007) Application of isoprinosine in ovo to immunomodulation in turkeys. XIX International Poultry Symposium Polish Branch WPSA, Olsztyn, 10-12 September, p. 63.
• Koncicki A, Tykalowski B, Stenzel T, Andrzejewski M (2009) Assessment of the efficiency of applying me­thisoprinol and vaccination of turkeys against the hem- morrhagic enteritis virus using in ovo methods. Bull Vet Inst Puławy 53: 13-15.
• Krasnodębska-Depta A, Koncicki A (2000) Physiological values of selected serum biochemical indices in broiler chickens. Med Weter 56: 456-460.
• Maiaczewska J, Rotkiewicz Z, Siwicki AK (2003) Effect of methisoprinol and KLP-602 on the development of im­munocompetent organs and selected biochemical indices of allantoic fluid of chicken embryos. Pol J Vet Sci 6: 21-24.
• Maiaczewska J, Rotkiewicz Z (2004) Effect of methisoprinol on virus replication in cell cultures. Pol J Vet Sci 7: 97-102.
• Maiaczewska J, Rotkiewicz Z (2005) Biological properties of Roakin strain of NDV and TK900 strain of ADV after serial passages in CECC in the presence of methisoprinol and KLP-602. Pol J Vet Sci 8: 23-28.
• Mazur-Gonkowska B, Koncicki A, Krasnodębska-Depta A (2004) Assessment of acute phase response in turkeys experimentally infected with Escherichia coli or haemor­rhagic enteritis virus. Bull Vet Inst Puławy 48: 19-23.
• Moore CB, Siopes TD (2005) Enhancement of cellular and humoral immunity following embryonic exposure to melatonin in turkeys (meleagridis gallopavo). General Comp Endocrinol 143: 178-183.
• Moya P, Alonso ML, Baixeras E, Ronda E (1984) Im­munomodulatory activity of isoprinosine on experimental viral infections in avian models. Int J Immunopharmacoi 6: 339-343.
• Parry RM, Chandon RC, Shahani KM. (1965) A rapid and sensitive assay of muramidase. Proc Soc Exp Biol Med 119: 384-386.
• Rautenschlein S, Sharma JM, Winslow BJ, McMillen J, Junker D, Cochran M (2000) Embryo vaccination of tur­keys against Newcastle disease infection with recom­binant fowl pox virus constructs containing interferons as adjuvants. Vaccine 18: 426-433.
• Rautenschlein S, Neumann U (2004) Modulation of avian immune system: suppression versus induction of immun­ity. Pol J Vet Sci 7: 39-46.
• Riterich R (1971) Chemia kliniczna. PZWL Warszawa.
• Rumińska-Groda E (2002) Wpływ izoprynozyny na dynamikę zjawisk immunologicznych oraz przebieg zakażenia wirusem HE i pałeczkami E. coli u indyków. Doctoral thesis, Olsztyn, Poland.
• Sharma JM (1997) The structure and function of the avian immune system. Acta Vet Hungar 45: 229-238.
• Sharma J (1999) Introduction to poultry vaccines and im­munity. Adv Vet Med 41: 481-494.
• Siegel P (2003) The role of genetic resistance in poultry and consumer protection. Turkey Production: Balance act be­tween consumer protection, animal welfare and econ­omic aspects. 5th International Symposium on Turkey Diseases, Berlin, pp 6-12.
• Szabo A, Mezes M, Horn P, Suto Z, Bazar G, Romvari R (2005) Developmental dynamics of some blood bi­chemical parameters in growing turkey (Meleagridis gal­lopavo). Acta Vet Hungar 53: 397-409.
• Stenzel T, Tykalowski B, Andrzejewski M, Koncicki A (2008) The influence of methisoprinol applied in ovo on the percentage of CD3+, CD4+ and CD8+ T-lym- phocyte subpopulations in turkeys. Med Weter 64: 1157- -1160.
• Worthington KJ, Sergeant BA, Davelaar FG, Jones RC (2003) Immunity to avian pneumovirus infection in tur­keys following in ovo vaccination with an attenuated vac­cine. Vaccine 21: 1355-1362.
• Wójcik R, Święcicka-Grabowska G (2003) Biochemical and non-specific immunity parameters in turkeys immunised with the Roakin strain of NDV virus after administrating levamisol and isoprinozine. Med Weter 59: 713-715.