Profile bialkowe plazmy nasienia lisa polarnego na poczatku i na koncu sezonu reprodukcyjnego

• Autorzy: Stasiak K , Janicki B. , Kowalski R. , Glogowski J.

Warianty tytułu

EN

Protein profile of the Polar fox's semen plasma at the beginning and at the end of the reproductive season

• Języki publikacji: PL

Abstrakty

EN

The aim of the studies was to assess the protein composition of the polar fox’s (Alopex lagopus L.) semen plasma with the use of the electrophoretic method (SDS-PAGE) and to find out whether there are statistically important differences in the protein profiles between the beginning and the end of the reproductive period and between the selected proteins and the semen traits. The examined material was the semen plasma obtained with the use of the manual method from nine nine-year-old polar foxes. Both in the plasma obtained at the beginning and at the end of the season ten protein fractions (10.9÷107.5 kDa) were distinguished. On the basis of the densitometric analysis of electrophoretic images, a quantitative decrease of multimolecular forms at the end of the reproductive period was observed. This may indicate either a decreasing synthesis of proteins in the reproductive system or partial proteolysis in progress. The analysis of correlations, in turn, indicated significant relationships (p < 0.05) between the masses of protein fractions (107.5 and 58.9 kDa) and the volume of the semen collected at the beginning of the season (r = -0.76 and r = -0.72 respectively): between proteins 74.8 and 34.9 kDa and the volume of the semen at the end of the season (r = 0.92 and r = 0.79 respectively), and also between proteins 58.9; 34.9; 27.0 and the percentage of the sperm with major flaws in the semen collected at the beginning of the reproductive season (r = 0.69; r = 0.89 and r= -0.76 respectively). Even though significant correlations between the plasma proteins and the traits of the semen obtained from young (one-year-old) individuals were observed, it is necessary to continue the research with reference to older animals (two-year-old foxes).

Słowa kluczowe

PL

bialka plazmy nasienia; zmiany; sezon rozrodczy; lisy polarne; profile bialkowe; poczatek sezonu; rozrod zwierzat; nasienie; elektroforeza; plazma nasienia; koniec sezonu

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2008
• Tom: 64
• Numer: 11
• Strony: 1340-1343
• Opis fizyczny: s.1340-1343,fot.,tab.,bibliogr.

Twórcy

autor

Stasiak K

• Uniwersytet Technologiczno-Przyrodniczy w Bydgoszczy, ul.Mazowiecka 28, 85-024 Bydgoszcz

autor

Janicki B.

autor

Kowalski R.

autor

Glogowski J.

Bibliografia

• 1.Arangasamy A., Singh L. P., Ahmed N., Ansari M. R., Ram G. C.: Isolation and characterization of heparin and gelatin binding buffalo seminal plasma proteins and their effect on cauda epididymal spermatozoa. Anim. Reprod. Sci. 2005, 90, 243-254.
• 2.Janicki B., Solińska J., Łakota P.: Ocena jakości i wartości biologicznej nasienia lisów polarnych w okresie sezonu rozrodczego. Medycyna Wet. 2006, 62, 1076-1079.
• 3.Jonáková V., Maňásková P., Tichá M.: Separation, characterization and identification of boar seminal plasma proteins. J. Chromat. B2007, 849, 307-314.
• 4.Kordan W., Strzeżek J., Soliwoda D., Celewicz M., Mogielnicka G.: Mapowanie białek plazmy nasienia knura przy wykorzystaniu metody elektroforezy dwukierunkowej w żelu poliakryloamidowym. Medycyna Wet. 2008, 64, 223-226.
• 5.Laemmli U. K.: Cleavage of structural proteins duringthe assembly of the head bacteriophage T4, Nature 1970, 227, 680-685.
• 6.Lowry O. H., Rosebrough N. J., Faar A. L., Randall R. J.: Protein measurement with the Folin phenol reagent. J. Biol. Chem. 1951, 193, 265-275.
• 7.Maňásková P., Liberda J., Tichá M., Jonáková V.: Isolation of non-heparin-binding and heparin-binding proteins of boar prostate. J. Chromat. B 2002, 770, 137-143.
• 8.Souza F. F., Barreto C. S., Lopes M. D.: Characteristics of seminal plasma proteins and their correlation with canine semen analysis. Theriogenology 2007, 68, 100-106.
• 9.Souza F. F., Martins M. I. M., Lopes M. D.: Vasectomy effect of canine seminal plasma biochemical components and their correlation with seminal parameters. Theriogenology 2006, 66, 1621-1625.
• 10.Souza F. F., Martins M. I. M., Santos Fernandes C. E., Ribolla P. E. M., Lopes M. D.: Heparin-binding proteins of canine seminal plasma. Theriogenology 2006, 66, 1606-1609.
• 11.Stasiak K., Janicki B.: Aktywność inhibitora trypsyny w plazmie nasienia lisa polarnego w sezonie rozrodczym. Medycyna Wet. 2007, 63, 990-993.
• 12.Strzeżek J.: Plazma nasienia a niektóre funkcje biologiczne plemników. Post. Biol. Kom. 1999, 26, Supl. 12, 59-68.
• 13.Strzeżek R., Janowski T.: Markery enzymatyczne gruczołu krokowego psa. Medycyna Wet. 2003, 59, 6-9.
• 14.Troedsson M. H., Desvousges A., Alghamdi A. S., Dahms B., Dow C. A., Hayna J., Valesco R., Collahan P. T., Macpherson M. L., Pozor M., Buhi W. C.: Components in seminal plasma regulating sperm transport and elimination. Anim. Reprod. Sci. 2005, 89, 171-186.