Modulated human globin gene expression: role for antisense expression vectors

• Autorzy: Xu L. , Pace B.S.
• Języki publikacji: EN

Abstrakty

EN

Several approaches have been explored to prevent polymerization of sickle hemoglobin in erythrocytes. We tested the ability of a mammalian expression vector carrying a β gene antisense cDNA fragment to block β gene expression. The antisense expression vector was stably transfected into HS2γβ stable mouse erythroleukemia cell lines producing human γ and β globin chains. By day 14 there was an average 24% decrease and 12% increase in β and γ globin mRNA levels respectively. We observed a loss of β gene inhibition by day 42. This study suggests that a β globin antisense cDNA expression vector may be an alternative gene therapy strategy to decrease sickle hemoglobin levels in patient with sickle cell disease.

Słowa kluczowe

EN

hemoglobin; antisense expression vector; man; gene expression; human globin; erythrocyte; mRNA; sickle-cell disease; cDNA; oligodeoxynucleotide

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 1998
• Tom: 03
• Numer: 4
• Opis fizyczny: p.423-433,fig.

Twórcy

autor

Xu L.

• University of South Alabama, Mobile, AL 36688, USA

autor

Pace B.S.

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