Specific binding sites for progesterone and 17beta-estradiol in cells of Triticum aestivum L.

• Autorzy: Janeczko A. , Budziszewska B. , Skoczowski A. , Dybala M.
• Języki publikacji: EN

Abstrakty

EN

The presence and location of specific binding sites for progesterone and 17β-estradiol in cells of wheat were estimated using radioligand binding assay. Membrane and cytosolic fractions of non-vernalized and vernalized plants were tested using tritium-labelled ligands. Specific binding of [3H]progesterone and [3H]17β-estradiol occurs in wheat cells. The binding sites are located in membranes and in the cytosol. Specific binding of [3H]17β-estradiol is higher in the membranes than in the cytosol. Specific binding of both ligands in the cytosolic fraction is higher in vernalized plants than in non-vernalized ones. The possibility of the occurrence of steroid binding proteins specific for progesterone and 17β-estradiol, putative steroid receptors for these steroids in Triticum aestivum L., is discussed.

Słowa kluczowe

EN

Triticum aestivum; progesterone; 17beta-estradiol; cell; vernalization; plant growth; plant development; signalling molecule

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2008
• Tom: 55
• Numer: 4
• Strony: 707-711
• Opis fizyczny: p.707-711,fig.,ref.

Twórcy

autor

Janeczko A.

• Polish Academy of Sciences, Niezapominajek 21, 30-239 Krakow, Poland

autor

Budziszewska B.

autor

Skoczowski A.

autor

Dybala M.

Bibliografia

• Beato M, Herrlich P, Schutz G (1995) Steroid hormone receptors: Many factors in search of a plot. Cell 83:851-857.
• Bordin S, Petra PH (1980) Biochemistry immunocytochemical localization of the sex steroid-binding protein of plasma in tissues of the adult monkey Macaca nemestrina. Proc Natl Acad Sci USA 77:5678-5682.
• Cenedella RJ, Sexton PS, Zhu XL (1999) Lens epithelia contain a high-affinity, membrane steroid hormone-binding protein. Invest Ophthalmol Vis Sci 40:1452-1459.
• Chow B, McCourt P (2006) Plant hormone receptors: perception is everything. Genes Develop 20:1998-2008.
• Garofalo EG, Raymondo SG (1995) Effect of oxytocin on estrogen and progesterone receptors in the rat uterus. Vet Res 26:284-291.
• Hammond B, Katzenellenbogen BS, Krauthammer N, McConnell J (1979) Estrogenic activity of the insecticide chlordecone (Kepone) and interaction with uterine estrogen receptors. Proc Natl Acad Sci USA 76:6641-6645.
• Holinka CF, Bressler RS, Zehr DR, Gurpide E (1980) Comparison of effects of estertrol and tamoxifen with those of estriol and estradiol on the immature rat uterus. Biol Reprod 22:913-926.
• Iino M, Nomura N, Tamaki Y, Yamada Y, Yoneyama K, Takeuchi Y, Mori M, Asami T, Nakano T, Yokota T (2007) Progesterone: its occurrence in plants and involvement in plant growth. Phytochemistry 68:1664-1673.
• Janeczko A, Filek W (2002) Stimulation of generative development in partly vernalized winter wheat by animal sex hormones. Acta Physiol Plant 24:291-295.
• Janeczko A, Filek W, Skoczowski A (2002) Influence of human sex hormones on the growth response of winter wheat immature embryos and callus. Zesz Probl Post Nauk Roln 488:667-673 (in Polish).
• Janeczko A, Skoczowski A (2005) Mammalian sex hormones in plants. Folia Histochem Cytobiol 43:71-79.
• Janik JR, Adler JH (1984) Estrogen receptors in Gladiolus ovules. Plant Physiol 75 S:135.
• Li JM, Jin H (2007) The regulation of brassinosteroids signaling. Trends Plant Sci 12:37-41.
• Marcinkowska E, Wiedlocha A (2002) Steroid signal transduction activated at the cell membrane: from plants to animals. Acta Biochim Polon 49:735-745.
• Milanesi L, Boland R (2004) Presence of estrogen receptor (ER)-like proteins and endogenous ligands for ER in solanaceae. Plant Sci 166:397-404.
• Norman AW, Mizwicki MT, Norman DPG (2004) Steroid-hormone rapid actions, membrane receptors and a conformational ensemble model. Nat Rev Drug Discov 3:27-41.
• Nowak G (1998) Methods and data analyses in radioligand-receptor binding: unnecessary transformations may cause biased results. Pol J Pharmacol 50:101-105.
• Robinson-Rechavi M, Garcia HE, Laudet V (2003) The nuclear receptor superfamily. J Cell Sci 116:585-586.
• Sedmak JJ, Grossberg SE (1977) A rapid, sensitive and versalite assay for protein using Coomassie Brilliant Blue G250. Anal Biochem 79:544-552.
• Sembdner G, Atzorn R, Schneider G (1994) Plant hormone conjugation. Plant Mol Biol 26:1459-1481.
• Simons RG, Grinwich DL (1989) Immunoreactive detection of four mammalian steroids in plants. Can J Bot 67:288-296.
• Sommarin M, Lundborg T, Kylin A (1985) Comparison of K, Mg ATPases in purified plasmalemma from wheat and oat. Substrate specificities and effect of pH, temperature and inhibitors. Physiol Plant 65:27-32.
• Stanczyk FZ, Nakamura RM, Novy MJ (1989) Disparity in the response of sex steroid-binding protein and corticosteroid-binding globulin to thyroxine in the primate. Biol Reprod 41:63-67.
• Wang ZY, Wang QM, Chong K, Wang L, Bai MY, Jia CG, (2006) The brassinosteroid signal transduction pathway. Cell Res 16:427-434.
• Yang XH, Xu ZH, Xue HW (2005) Arabidopsis membrane steroid binding protein 1 is involved in inhibition of cell elongation. Plant Cell 17:116-131.
• Zhu Y, Rice CD, Pang YF, Pace M, Thomas P (2003) Cloning, expression, and characterization of a membrane progestin receptor and evidence it is an intermediary in meiotic maturation of fish oocytes. Proc Natl Acad Sci USA 100:2231-2236.