Incidence and prevalence of infection with Anaplasma phagocytophilum. Prospective study in healthy individuals exposed to ticks

• Autorzy: Grzeszczuk A. , Puzanowska B. , Miegoc H. , Prokopowicz D.
• Języki publikacji: EN

Abstrakty

EN

The seroprevalence of human granulocytic anaplasmosis (former human granulocytic ehrlichiosis, HGE) has been documented in several studies, but little data exists on incidence rates in healthy individuals. In a prospective study, we tested 125 healthy adults (mean age 43 years) - workers of the Białowieża Primeval Forest National Park, north-eastern Poland - for Anaplasma phagocytophilum IgG antibodies using an indirect immunofluorescence antibody assay, and for Borrelia burgdorferi IgG with ELISA in a 12-month interval. The data concerning clinical symptoms consistent with human granulocytic anaplasmosis were collected using a standardized questionnaire. Of these 125 subjects, 9 were anti-A. phagocytophilum positive at the study entry. Four participants (3.2%) seroconverted from IgG negative to positive during the observation period. Three subjects (2.4%) converted from initially anti-A. phagocytophilum positive to negative. Specific IgG antibodies against Borrelia burgdorferi were detected in 27 (21.6%) individuals. Concurrence of Borrelia burgdorferi and Anaplasma phagocytophilum was observed in 3.2%, whereas 4% were Anaplasma phagocytophilum IgG positive and Borrelia burgdorferi IgG negative (not significant). Clinical symptoms associated with human granulocytic anaplasmosis were not present in seroconverting individuals. The obtained results confirm the occurrence of Anaplasma phagocytophilum infection in north-eastern Poland with asymptomatic clinical course.

Słowa kluczowe

EN

infection; man; clinical symptom; Poland; Anaplasma phagocytophilum; Borrelia burgdorferi; infectious disease; antibody; tick

• Wydawca: -
• Czasopismo: Annals of Agricultural and Environmental Medicine
• Rocznik: 2004
• Tom: 11
• Numer: 1
• Opis fizyczny: p.155-157,ref.

Twórcy

autor

Grzeszczuk A.

• Medical University of Bialystok, Zurawia 14, 15-540 Bialystok, Poland

autor

Puzanowska B.

autor

Miegoc H.

autor

Prokopowicz D.

Bibliografia

• 1. Aguero-Rosenfeld ME, Kalantarpour F, Baluch M, Horowitz HW, McKenna DF, Raffalli JT, Hsieh T, Wu J, Dumler JS, Wormser GP: Serology of culture-confirmed cases of human granulocytic ehrlichiosis. J Clin Microbiol 2000, 38, 635-638.
• 2. Aguero-Rosenfeld ME: Diagnosis of human granulocytic ehrlichiosis: state of the art. Vector Borne Zoonotic Dis 2002, 2, 233-239.
• 3. Bakken JS, Haller I, Riddell D, Walls JJ, Dumler JS: The serological response of patients infected with the agent of human granulocytic ehrlichiosis. Clin Infect Dis 2002, 34, 22-27.
• 4. Blanko JR, Oteo JA: Human granulocytic ehrlichiosis in Europe. Clin Microbiol Infect 2002, 8, 763-72.
• 5. Chen SM, Dumler JS, Bakken JS, Walker DH: Identification of a granulocytoropic Ehrlichia species as the etiologie agent of human disease. J Clin Microbiol 1994, 32, 589-95.
• 6. Cisak E, Chmielewska-Badora J, Rajtar В, Zwoliński J, Jabłoński L, Dutkiewicz J: Study on the occurrence of Borrelia burgdorferi sensu lato and tick-borne encephalitis virus (TBEV) in ticks collected in Lublin region (eastern Poland). Ann Agric Environ Med 2002, 9, 105-110.
• 7. Derdáková M, Halánová M, Stanko M, Štefančiková A, Čisláková L, Peťko B: Molecular evidence for Anaplasma phagocytophilum and Borrela burgdorferi sensu lato in Ixodes ricinus ticks from eastern Slovakia. Ann Agric Environ Med 2003, 10, 269-271.
• 8. Dumler JS, Walker HD: Tick-borne ehrlichioses. Lancet Infect Dis 2001, 0, 21-28 (preview edition).
• 9. Grzeszczuk A, Stańczak J, Kubica-Bierant B, Kruminis-Łozowska W, Racewicz M. First seroepidemiological evidence of human granulocytic ehrlichiosis (HGE) in Poland. Preliminary results. VIII European Multicolloquium of Parasitology (EMOP) Poznań. Acta Parasitol 2000, 45, 219-219 (abstract).
• 10. Grzeszczuk A, Stańczak J, Kubica-Biernat B. Serological and molecular evidence of human granulocytic ehrlichiosis focus in the Białowieża Primeval Forest (Puszcza Białowieska), north-eastern Poland. Eur J Clin Microbiol Infect Dis 2002, 21, 6-11.
• 11. Hilton E, DeVoti J, Benach JL, Halluska M, White DJ, Paxton H, Dumler SJ: Seroprevalence and serocnoversion for tick-borne diseases in a high-risk polulation in the northeast United States. Am J Med 1999, 106, 404-409.
• 12. Niścigorska J, Skotarczak B, Wodecka В: Borrelia burgdorferi infection among forestry workers - assessed with an immunoenzymatic method (ELISA), PCR and correlated with the clinical state of the patients. Ann Agric Environ Med 2003, 10, 15-19.
• 13. Ravyn MD, Goodman JL, Kodner CB, Westand DK, Coleman LA, Engstrom SM, Nelson CM, Johnson RC: Immunodiagnosis of human granulocytic ehrilichiosis by using culture-derived human isolates. J Clin Microbiol 1998, 36, 1480-1488.
• 14. Stańczak J, Kubica-Bierant B, Kruminis-Łozowska W, Racewicz M: Detection of the agent of human granulocytic ehrlichiosis (HGE) in Poland. VIII European Multicolloquium of Parasitology (EMOP). Acta Parasitol 2000, 45, 217-17 (abstract).
• 15. Stańczak J, Racewicz M, Kubica-Biernat B, Kruminis-Łozowska W, Dąbrowski J, Adamczyk A, Markowska M: Prevalence of Borrelia burgdorferi sensu lato in Ixodes ricinus ticks (Acari, Ixodidae) in different Polish woodlands. Ann Agric Environ Med 1999, 6, 127-132.
• 16. Tylewska-Wierzbanowska S, Chmielewski T, Kondrusik M, Hermanowska-Szpakowicz T, Sawicki W, Sułek K: First cases of acute human granulocytic ehrlichiosis in Poland. Eur J Clin Microbiol Infect Dis 2001,20, 196-8.
• 17. Wittesjö В, Bjöersdorff A, Eliasson I, Berglund J: First long-term study of the seroresponse to the agent of human granulocytic ehrlichiosis among residents of a tick-endemic area of Sweden. Eur J Clin Microbiol Infect Dis 2001,20, 173-178.
• 18. Woessner R, Gaertner BC, Grauer MT, Weber K, Mueller- Lantzsch N, Hunfeld KP, Treib J: Incidence and prevalence of infection with human granulocytic ehrlichiosis agent in Germany. A prospective study in young healthy subjects. Infection 2001, 29, 271-273.