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Abstrakty
Otherwise isogenic Escherichia coli CP78 (relA+) and CP79 (relA-) strains are commonly used in studies on the stringent control, the bacterial response to amino acid starvation. We found that these strains are lysogenic for a phage which is spontaneously induced with a low frequency, producing virions able to infect other E. coli strains. Genetic studies, restriction analysis of the phage DNA genome, and electron microscopy revealed that this phage is very similar to, but not identical with, bacteriophage λ. We called the newly isolated phage λW, and found that most of CP78/CP79 ancestor strains are lysogenic for this phage.
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p.251-259,fig.
Twórcy
autor
- University of Gdansk, Kladki 24, 80-822 Gdansk, Poland
autor
autor
Bibliografia
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- 3. Hernandez, V.J. & Bremer, H. (1991) Escherichia coli ppGpp synthetase II activity requires spoT. J. BioL Chem. 266, 5991-5999.
- 4. Xiao, H., Kaiman, M., Ikehara, K., Zemel, S., Glaser, G. & Cashel, M. (1991) Residual guano- sine 3',5'-bispyrophosphate synthetic activity of relA null mutants can be eliminated by spoTnull mutations. J. BioL Chem. 266, 5980- 5990.
- 5. Gentry, D.R. & Cashel, M. (1996) Mutational analysis of the Escherichia coli spoT gene identifies distinct but overlapping regions involved in ppGpp synthesis and degradation. Mol Microbiol 19, 1373-1384.
- 6. Murray, K.D. & Bremer, H. (1996) Control of spoT-dependsnt ppGpp synthesis and degradation in Escherichia coli. J. Mol Biol 259, 41-57.
- 7. Fiil, N. & Friesen, J.D. (1968) Isolation of relaxed mutants of Escherichia coli. J. Bacteriol 95, 729-731.
- 8. Bachmann, B.J. (1996) Derivations and genotypes of some mutant derivatives of Escherichia coli K-12; in Escherichia coli and Salmonella: Cellular and Molecular Biology (Neid- hardt, F.C., Curtiss, III, R., Ingraham, J.L., Lin, E.C.C., Low, K.B., Magasanik, B., Reznik- off, W.S., Riley, M., Schaechter, M. & Um- barger, H.E., eds.) pp. 2460-2488, American Society for Microbiology, Washington, DC.
- 9. Campbell, A. & Botstein, D. (1983) Evolution of the lambdoid phages; in Lambda II (Hen- drix, R.W., Roberts, J.W., Stahl, F.W. & Weis- berg, R.A., eds.) pp. 365-380, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
- 10. Goldberg, A.R. & Howe, M. (1969) New mutations in the 5 cistron of bacteriophage lambda affecting host cell lysis. Virology 38,200-202.
- 11. Kur, J., Górska, I. & Taylor, K. (1987) Escherichia coli dnaA initiator function is required for replication of plasmids derived from coliphage lambda. J. Mol. Biol 198, 203-210.
- 12.Szalewska, A., Węgrzyn, G. & Taylor, K. (1994) Neither absence nor excess of A O initiator-digesting ClpXP protease affects A plasmid or phage replication in Escherichia coli. Mol Microbiol 13, 469-474.
- 13. Arber, W., Enquist, L., Hohn, B., Murray, N.E. & Murray, K. (1983) Experimental methods for use with lambda; in Lambda 11 (Hendrix, R.W., Roberts, J.W., Stahl, F.W. & Weisberg, R.A., eds.) pp. 433-466, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
- 14. Sambrook, J., Fritsch, E.F. & Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
- 15. Obuchowski, M. & Stopa, M. (1993) Quick and efficient method for recovering of bacteriophages from soft agar after their propagation by the plate lysate technique. Acta Biochim. Polon. 40, 98-99.
- 16. Węgrzyn. G., Węgrzyn, A., Pankiewicz, A. & Taylor, K. (1996) Allele specificity of the Escherichia coli dnaA gene function in the replication of plasmids derived from phage A. Mol Gen. Genet. 252, 580-586.
- 17. Wischnitzer, S. (1981) Introduction to Electron Microscopy. Pergamon Press, New York, NY.
- 18. Bozzola, J.J. & Rusell, L.D. (1991) Electron Microscopy: Principles and Techniques for Biologists. Jones and Bartlett Publishers, Boston, MA.
- 19. Polissi, A., Goffin, L. & Georgopoulos, C. (1995) The Escherichia coli heat shock response and bacteriophage A development. FEMS Microbiol Rev. 17, 159-169.
- 20. Taylor, K. & Węgrzyn, G. (1995) Replication of coliphage lambda DNA. FEMS Microbiol Rev. 17, 109-119.
- 21. Szalewska-Pałasz, A., Węgrzyn, A., Herman, A. & Węgrzyn, G. (1994) The mechanism of the stringent control of A plasmid DNA replica- tion. EMBOJ. 13, 5779-5785.
- 22. Jensen, K.F. (1993) The Escherichia coli 'wild types' W3110 and MG1655 have an rph frame- shift mutation that leads to pyrimidine starvation due to lowpyrE expression levels. J. Bacteriol 175, 3401-3407.
- 23. Appleyard, R.K. (1954) Segregation of new ly- sogenic types during growth of a doubly lyso-genie strain derived from Escherichia coli Kl 2. Genetics 39, 440-452.
- 24. Georgopoulos, C.P. & Herskowitz, I. (1971) Escherichia coli mutants blocked in lambda DNA synthesis; in The Bacteriophage Lambda (Hershey, A.D., ed.) pp. 553-564, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
- 25. Węgrzyn, G., Wtegrzyn, A., Konieczny, I., Rie- lawski, K., Konopa, G., Obuchowski, M., Helin- ski, D.R. & Taylor, K. (1995) Involvement of the host initiator function dnaA in the replication of coliphage A. Genetics 139,1469-1481.
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Bibliografia
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bwmeta1.element.agro-article-4c122b42-954e-4576-a004-42f9e6332a3c