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1998 | 45 | 1 |
Tytuł artykułu

Biochemical and genetic analysis of lambda W, the newly isolated lambdoid phage

Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Otherwise isogenic Escherichia coli CP78 (relA+) and CP79 (relA-) strains are commonly used in studies on the stringent control, the bacterial response to amino acid starvation. We found that these strains are lysogenic for a phage which is spontaneously induced with a low frequency, producing virions able to infect other E. coli strains. Genetic studies, restriction analysis of the phage DNA genome, and electron microscopy revealed that this phage is very similar to, but not identical with, bacteriophage λ. We called the newly isolated phage λW, and found that most of CP78/CP79 ancestor strains are lysogenic for this phage.
Wydawca
-
Rocznik
Tom
45
Numer
1
Opis fizyczny
p.251-259,fig.
Twórcy
autor
  • University of Gdansk, Kladki 24, 80-822 Gdansk, Poland
autor
Bibliografia
  • 1. Cashel, M., Gentry, D.R., Hernandez, V.J. & Vinella, D. (1996) The stringent response; in Escherichia coli and Salmonella: Cellular and Molecular Biology (Neidhardt, F.C., Curtiss III, R., Ingraham, J.L., Lin, E.C.C., Low, K.B., Ma- gasanik, B., Reznikoff, W.S., Riley, M., Schae- chter, M. & Umbarger, H.E., eds.) pp. 1458-1496, American Society for Microbiol­ogy, Washington, DC.
  • 2. Reddy, P.S., Raghavan, A. & Chatterji, D. (1995) Evidence for ppGpp binding site on E. coli RNA polymerase: Proximity relationship with the rifampicin binding domain. Mol. Mi- crobioL 15, 255-2GG.
  • 3. Hernandez, V.J. & Bremer, H. (1991) Escheri­chia coli ppGpp synthetase II activity requires spoT. J. BioL Chem. 266, 5991-5999.
  • 4. Xiao, H., Kaiman, M., Ikehara, K., Zemel, S., Glaser, G. & Cashel, M. (1991) Residual guano- sine 3',5'-bispyrophosphate synthetic activity of relA null mutants can be eliminated by spoTnull mutations. J. BioL Chem. 266, 5980- 5990.
  • 5. Gentry, D.R. & Cashel, M. (1996) Mutational analysis of the Escherichia coli spoT gene iden­tifies distinct but overlapping regions involved in ppGpp synthesis and degradation. Mol Mi­crobiol 19, 1373-1384.
  • 6. Murray, K.D. & Bremer, H. (1996) Control of spoT-dependsnt ppGpp synthesis and degrada­tion in Escherichia coli. J. Mol Biol 259, 41-57.
  • 7. Fiil, N. & Friesen, J.D. (1968) Isolation of re­laxed mutants of Escherichia coli. J. Bacteriol 95, 729-731.
  • 8. Bachmann, B.J. (1996) Derivations and geno­types of some mutant derivatives of Escheri­chia coli K-12; in Escherichia coli and Salmo­nella: Cellular and Molecular Biology (Neid- hardt, F.C., Curtiss, III, R., Ingraham, J.L., Lin, E.C.C., Low, K.B., Magasanik, B., Reznik- off, W.S., Riley, M., Schaechter, M. & Um- barger, H.E., eds.) pp. 2460-2488, American Society for Microbiology, Washington, DC.
  • 9. Campbell, A. & Botstein, D. (1983) Evolution of the lambdoid phages; in Lambda II (Hen- drix, R.W., Roberts, J.W., Stahl, F.W. & Weis- berg, R.A., eds.) pp. 365-380, Cold Spring Harbor Laboratory Press, Cold Spring Har­bor, NY.
  • 10. Goldberg, A.R. & Howe, M. (1969) New muta­tions in the 5 cistron of bacteriophage lambda affecting host cell lysis. Virology 38,200-202.
  • 11. Kur, J., Górska, I. & Taylor, K. (1987) Escheri­chia coli dnaA initiator function is required for replication of plasmids derived from coliphage lambda. J. Mol. Biol 198, 203-210.
  • 12.Szalewska, A., Węgrzyn, G. & Taylor, K. (1994) Neither absence nor excess of A O initiator-digesting ClpXP protease affects A plasmid or phage replication in Escherichia coli. Mol Microbiol 13, 469-474.
  • 13. Arber, W., Enquist, L., Hohn, B., Murray, N.E. & Murray, K. (1983) Experimental methods for use with lambda; in Lambda 11 (Hendrix, R.W., Roberts, J.W., Stahl, F.W. & Weisberg, R.A., eds.) pp. 433-466, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
  • 14. Sambrook, J., Fritsch, E.F. & Maniatis, T. (1989) Molecular Cloning: A Laboratory Man­ual Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
  • 15. Obuchowski, M. & Stopa, M. (1993) Quick and efficient method for recovering of bacterio­phages from soft agar after their propagation by the plate lysate technique. Acta Biochim. Polon. 40, 98-99.
  • 16. Węgrzyn. G., Węgrzyn, A., Pankiewicz, A. & Taylor, K. (1996) Allele specificity of the Escherichia coli dnaA gene function in the rep­lication of plasmids derived from phage A. Mol Gen. Genet. 252, 580-586.
  • 17. Wischnitzer, S. (1981) Introduction to Electron Microscopy. Pergamon Press, New York, NY.
  • 18. Bozzola, J.J. & Rusell, L.D. (1991) Electron Mi­croscopy: Principles and Techniques for Biolo­gists. Jones and Bartlett Publishers, Boston, MA.
  • 19. Polissi, A., Goffin, L. & Georgopoulos, C. (1995) The Escherichia coli heat shock re­sponse and bacteriophage A development. FEMS Microbiol Rev. 17, 159-169.
  • 20. Taylor, K. & Węgrzyn, G. (1995) Replication of coliphage lambda DNA. FEMS Microbiol Rev. 17, 109-119.
  • 21. Szalewska-Pałasz, A., Węgrzyn, A., Herman, A. & Węgrzyn, G. (1994) The mechanism of the stringent control of A plasmid DNA replica- tion. EMBOJ. 13, 5779-5785.
  • 22. Jensen, K.F. (1993) The Escherichia coli 'wild types' W3110 and MG1655 have an rph frame- shift mutation that leads to pyrimidine starva­tion due to lowpyrE expression levels. J. Bacte­riol 175, 3401-3407.
  • 23. Appleyard, R.K. (1954) Segregation of new ly- sogenic types during growth of a doubly lyso-genie strain derived from Escherichia coli Kl 2. Genetics 39, 440-452.
  • 24. Georgopoulos, C.P. & Herskowitz, I. (1971) Escherichia coli mutants blocked in lambda DNA synthesis; in The Bacteriophage Lambda (Hershey, A.D., ed.) pp. 553-564, Cold Spring Harbor Laboratory Press, Cold Spring Har­bor, NY.
  • 25. Węgrzyn, G., Wtegrzyn, A., Konieczny, I., Rie- lawski, K., Konopa, G., Obuchowski, M., Helin- ski, D.R. & Taylor, K. (1995) Involvement of the host initiator function dnaA in the replica­tion of coliphage A. Genetics 139,1469-1481.
Typ dokumentu
Bibliografia
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Identyfikator YADDA
bwmeta1.element.agro-article-4c122b42-954e-4576-a004-42f9e6332a3c
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