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2006 | 47 | 4 |
Tytuł artykułu

DNA damage in mouse lymphocytes exposed to curcumin and copper

Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Dietary polyphenolics, such as curcumin, have shown antioxidant and anti-inflammatory effects. Some antioxidants cause DNA strand breaks in excess of transition metal ions, such as copper. The aim of this study was to evaluate the in vitro effect of curcumin in the presence of increasing concentrations of copper to induce DNA damage in murine leukocytes by the comet assay. Balb-C mouse lymphocytes were exposed to 50 µM curcumin and various concentrations of copper (10 µM, 100 µM and 200 µM). Cellular DNA damage was detected by means of the alkaline comet assay. Our results show that 50 µM curcumin in the presence of 100-200 µM copper induced DNA damage in murine lymphocytes. Curcumin did not inhibit the oxidative DNA damage caused by 50 µM H₂O₂ in mouse lymphocytes. Moreover, 50 µM curcumin alone was capable of inducing DNA strand breaks under the tested conditions. The increased DNA damage by 50 µM curcumin was observed in the presence of various concentrations of copper, as detected by the alkaline comet assay.
Wydawca
-
Rocznik
Tom
47
Numer
4
Opis fizyczny
p.377-382,fig.,ref.
Twórcy
  • Laboratorio de Citogenetica Genotoxicidad y Biomonitoreo, Instituto de Genetics Humana "Dr. Enrique Corona Rivera", Departamento de Biologia Molecular y Genomica, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Sierra Mojada 950, Colonia Independencia, S.L., CP-44340, Guadalajara, Jalisco, Mexico
  • Laboratorio de Citogenetica Genotoxicidad y Biomonitoreo, Instituto de Genetics Humana "Dr. Enrique Corona Rivera", Departamento de Biologia Molecular y Genomica, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Sierra Mojada 950, Colonia Independencia, S.L., CP-44340, Guadalajara, Jalisco, Mexico
  • Laboratorio de Neurofisiologia, Departamento de Fisiologia, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Sierra Mojada 950, Colonia Independencia, S.L., CP-44340, Guadalajara, Jalisco, Mexico
  • Laboratorio de Citogenetica Genotoxicidad y Biomonitoreo, Instituto de Genetics Humana "Dr. Enrique Corona Rivera", Departamento de Biologia Molecular y Genomica, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Sierra Mojada 950, Colonia Independencia, S.L., CP-44340, Guadalajara, Jalisco, Mexico
  • Laboratorio de Neurofisiologia, Departamento de Fisiologia, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Sierra Mojada 950, Colonia Independencia, S.L., CP-44340, Guadalajara, Jalisco, Mexico
  • Laboratorio de Citogenetica Genotoxicidad y Biomonitoreo, Instituto de Genetics Humana "Dr. Enrique Corona Rivera", Departamento de Biologia Molecular y Genomica, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Sierra Mojada 950, Colonia Independencia, S.L., CP-44340, Guadalajara, Jalisco, Mexico
  • Laboratorio de Citogenetica Genotoxicidad y Biomonitoreo, Instituto de Genetics Humana "Dr. Enrique Corona Rivera", Departamento de Biologia Molecular y Genomica, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Sierra Mojada 950, Colonia Independencia, S.L., CP-44340, Guadalajara, Jalisco, Mexico
  • Unidad de Citogenetica, OPD Hospital Civil "Fray Antonio Alcalde", Guadalajara, Jalisco, Mexico
Bibliografia
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Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-article-48f95ae9-f0aa-4c52-81a2-498734187aa9
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