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Abstrakty
Kentucky bluegrass is a facultative apomict which is propagated from seeds of variable genetic origins, ploidy levels and nuclear DNA contents. This study analyzes the variability of relative nuclear DNA content among cultivars and natural populations, and examines whether this variability is correlated with morphological traits. Relative nuclear DNA content (an indirect measure of chromosomal variability) was determined in 281 plants from 28 accessions (17 cultivars, 11 populations) using flow cytometry of DAPI-stained nuclei. The same plants were also measured for leaf area and stomatal length. Variation of measured relative DNA content between the studied accessions was very high (5.5-fold). Intra-accession variation was very high in six accessions, even though three of these were cultivars. Relative nuclear DNA content was correlated with stomatal length but not with leaf area. The lack of correlation with leaf area might explain why high intra-accession variability of nuclear DNA content was found in released cultivars that had passed uniformity testing during the registration procedure. We suggest that nuclear DNA content measurement should be made part of the cultivar registration process
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Tom
Numer
Strony
45-52
Opis fizyczny
p.45-52,fig.,ref.
Twórcy
autor
- University of Ljubljana, Jamnikarjeva 101, SI-1000 Ljubljana, Slovenia
autor
autor
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Bibliografia
- Anonymous. 1990. Guidelines for the conduct of tests for distinctness, homogeneity and stability. Kentucky bluegrass (Poa pratensis L.). Geneva: International Union for the Protection of New Varieties of Plants, Switzerland.
- Anonymous. 2002. General introduction to the examination of distinctness, uniformity and stability and the development of harmonized descriptions of new varieties of plants. Geneva: International Union for the Protection of New Varieties of Plants, Switzerland.
- Arumuganathan K, and Earle ED. 1991. Nuclear DNA content of some important plant species. Plant Molecular Biology Reporter 9: 208-218.
- Barcaccia G, Mazzucato A, Belardinelli A, Pezzotti M, Lucretti S, and Falcinelli M. 1997. Inheritance of parental genomes in progenies of Poa pratensis L. from sexual and apomictic genotypes as assessed by RAPD markers and flow cytometry. Theoretical and Applied Genetics 95: 516-524.
- Bennett MD and Smith dB. 1991. Nuclear DNA amounts in angiosperms. Philosophical Transactions of the Royal Society of London 334: 309-345.
- Bohanec B. 2003. Ploidy determination using flow cytometry. In: Maluszynski M, Kasha KJ, Forster BP and Szarejko I [eds.], Doubled Haploid Production in Crop Plants, 397-403. Kluwer Academic Publishers, Dordrecht.
- Caceres ME, De Pace C, Scarascia Mugnozza GT, Kotsonis P, Ceccarelli M, and Cionini PG. 1998. Genome size variations within Dasypyrum villosum: correlations with chromosomal traits, environmental factors and plant phenotypic characteristics and behaviour in reproduction. Theoretical and Applied Genetics 96: 559-567.
- Chung J, Lee JH, Arumuganathan K, Graef, and Specht JE. 1998. Relationships between nuclear DNA content and seed and leaf size in soybean. Theoretical and Applied Genetics 96: 1064-1068.
- Eaton TD, Curley J, Williamson RC, and Jung G. 2004. Determination of the level of variation in polyploidy among Kentucky bluegrass cultivars by means of flow cytometry. Crop Science 44: 2168-2174.
- Greilhuber J. 1998. Intraspecific variation in genome size: a critical reassessment. Annals of Botany 82: 27-35.
- Huff DR and Bara JM. 1993. Determining genetic origin of aberrant progeny from facultative apomictic Kentucky bluegrass using a combination of flow cytometry and silver-stained RAPD markers. Theoretical and Applied Genetics 87: 201-208.
- Ozias-Akins P. 2006. Apomixis: Developmental characteristics and genetics. Critical Reviews in Plant Sciences 25: 199-214.
- Stephens Le, Fei SZ, Xiong Y and Hodges CF. 2006. Plants regenerated from embryo cultures of an apomictic clone of Kentucky bluegrass (Poa pratensis L. 'Baron') are not apomictic in origin. Euphytica 147: 383-388.
- Sugiyama S, Yamaguchi K, and Yamada T. 2002. Intraspecific phenotypic variation associated with nuclear DNA content in Lolium perenne L. Euphytica 128: 145-151.
- Tatum TC, Nunez L, Kushad MM, and Rayburn AL. 2006. Genome size variation in pumpkin (Cucurbita sp.). Annals of Applied Biology 149: 145-151.
- Vilhar B, Vidic T, Jogan N, and Dermastia M. 2002. Genome size and the nucleolar number as estimators of ploidy level in Dactylis glomerata in the Slovenian Alps. Plant Systematics and Evolution 234: 1-13.
- Wieners RR, Fei SZ, and Johnson RC. 2006. Characterization of a USDA Kentucky bluegrass (Poa pratensis L.) core collection for reproductive mode and DNA content by flow cytometry. Genetic Resources and Crop Evolution 53: 1531-1541.
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Bibliografia
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