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Od pacjentów hospitalizowanych w 2001 roku wyhodowano 225 szczepów bezwzględnych beztlenowców, w tym 54 szczepy bakterii Gram-ujemnych i 171 szczepów bakterii Gram-dodatnich. Wśród Gram-ujemnych bakterii dominowały szczepy Bacteroides fragilis, a wśród Gram-dodatnich szczepy Peptostreptococcus sp. Stwierdzono, że lekami najbardziej aktywnymi wobec klinicznych szczepów Gram-ujemnych bakterii beztlenowych były: metronidazol, piperacylina z tazobaktamem, tikarcylina z kwasem klawulanowym i imipenem, a wobec szczepów Gram-dodatnich bakterii beztlenowych: piperacylina z tazobaktamem, amoksycyiina z kwasem klawulanowym, tikarcylina z kwasem klawulanowym i imipenem.
The aim of this study was to identify anaerobic strains isolated in 2001 from clinical specimens obtained from patients of Warsaw hospital and to evaluate a susceptibility of these strains to antimicrobial agents. In 2001 two hundred and twenty five clinical strains of obligate anaerobes were cultured, which were identified in the automatic ATB system (bioMérieux, France) using biochemical tests API 20 A. Drug-susceptibility of strains was determined also in ATB system with the use of ATB ANA strips. C. difficile strains were isolated on selective CCCA medium. Toxins A/B of C. difficile directly in stool specimens were detected by means of ELISA test (TechLab, USA). Fifty four strains of Gram-negative anaerobes (B. fragilis strains dominated) and 171 of Gram-positive anaerobes (the greatest number of strains belonged to genus Peptostrptococcus) were cultured from clinical specimens. In the cases of antibiotic-associated diarrhea 28 C. difficile strains were isolated and C. difficile toxins A/B were detected in 39 stool samples. The most active in vitro antimicrobials against Gram-negative anaerobes were metronidazole, imipenem, ticarcillin combined with clavulanic acid and piperacillin with tazobactam. Gram-positive, clinical strains of anaerobes were the most susceptible in vitro to ß-lactam antibiotics combined with ß-lactamase inhibitors (amoxicillin/clavulanate, piperacillin/tazobactam, ticarcillin/clavulanate) and imipenem.
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Strony
233-241
Opis fizyczny
s.233-241,tab.,bibliogr.
Twórcy
Bibliografia
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- 9. King A, DownesJ, Nord CE, Phillips I. Antimicrobial susceptibility of non-Bacteroides fragilis group anaerobic Gram-negative bacilli in Europe. Clin Microbiol Infect 1999; 5: 404-16.
- 10. Łuszczek A. Pałeczki rodzaju Bacteroides grupy Bactcroides fragilis: czynniki chorobotwórczości, lekowrażliwość oraz metody ich identyfikacji. Mikrobiologia Medycyna 1998; 3: 17-29.
- 11. National Committee for Clinical Laboratory Standards: Methods for antimicrobial susceptibility testing of anaerobic bacteria, 4th ed. Approved standard M11-A4. NCCLS, Villanova, PA, 1997.
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- 14. Rokosz A, Sawicka-Grzelak A, Kot K i inni. Zastosowanie metody Etest do oznaczania lekowrażliwości bakterii beztlenowych. Med Dośw Mikrobiol 2001; 53: 167-75.
- 15. Rokosz A, Sawicka - Grzelak A, Kot K i inni. Beta-laktamazy o rozszerzonym spektrum substratowym u Gram-ujemnych pałeczek rosnących w warunkach bezwzględnie beztleno#wych. Med Dośw Mikrobiol 2000; 52: 129-37.
- 16. Rokosz A, Sawicka-Grzelak A, Pituch H i inni. Detection of extended-spectrum ß-lactamases (ESBL) in clinical strains of Bactcroides fragilis. Antiinfect Drugs Chemother 1998; 16 (Suppl 1): 89.
- 17. Rotimi VO, Khoursheed M, Brazier JS i inni. Bactcroides species highly resistant to metronidazole: an emerging clinical problem? Clin Microbiol Infect 1999; 5: 166-69.
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- 19. Summanen P, Baron EJ, Citron DA i inni. Wadsworth anaerobic bacteriology manual, 5th ed. Star Publishing Company, Belmont, CA, 1993.
- 20. Wüst J, Hardegger U. Evaluation of the redesigned ATB ANA system (version 93) for susceptibility testing of anaerobic bacteria. Med Microbiol Lett 1995; 4: 68-75.
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Bibliografia
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