Inhibition of ergosterol biosynthesis in fungal plant pathogens by Bacillus sp.

• Autorzy: Czaczyk K , Trojanowska K. , Stachowiak B.
• Języki publikacji: EN

Abstrakty

EN

Antifungal activity of Bacillus coagulans creates the possibility to use this microorganism in biological control of fungal plant pathogens. Activity of Bacillus coagulans (No 6), isolated from lupine compost, against seven pathogenic species of indicator fungi: Bipolaris sorokiniana, Trichothecium roseum, Rhizoctonia solani, Sclerotinia sclerotiorum, Fusarium oxysporum, Fusarium solani and Fusarium culmorum were examined in this work. Ergosterol assessment and counting colony forming units (CFU) carried out determination of fungal growth. The addition of Bacillus coagulans to culture of fungi resulted in inhibition of ergosterol biosynthesis in mycelium. The strongest inhibition in all tested fungal plant pathogens was observed when Bacillus coagulans was co-inoculated at the beginning of culture. The decrease of ergosterol level did not always correlate with the decrease of CFU.

Słowa kluczowe

EN

antifungal activity; biological control; ergosterol; environment; biosynthesis; bacteria; fungi; chemical pesticide; Bacillus; plant pathogen; higher plant

• Wydawca: -
• Czasopismo: Polish Journal of Environmental Studies
• Rocznik: 2002
• Tom: 11
• Numer: 5
• Opis fizyczny: p.593-597,fig.

Twórcy

autor

Czaczyk K

• University of Agriculture, Wojska Polskiego 48, 60-627 Poznan, Poland

autor

Trojanowska K.

autor

Stachowiak B.

Bibliografia

• 1. AHIMOU F., JACQUES P., DELEU M. Surfactin and iturin A effects on Bacillus subtilis surface hydrophobicity. Enz. Microb. Technol. 27, 749, 2000.
• 2. CZACZYK K., STACHOWIAK B., TROJANOWSKA K., GULEWICZ K. Antifungal activity of Bacillus sp. isolated from compost. Folia Microb. 45, 552, 2000.
• 3. DAVIS M. W., LAMAR R. T. Evaluation of methods to extract ergosterol for quantification of soil fungal biomass. Soil Biol. Biochem. 24, 189, 1992.
• 4. DJAJAKIRANA G., JOERGENSEN R .G., MEYER B. Ergosterol and microbial biomass relationship in soil. Biol. Fertil. Soils 22, 299, 1996.
• 5. GOUARMA H., BULLERMAN L. B. Detection of moulds in foods and feeds: potential rapid and selective methods. J. Food Prot. 58, 1389, 1995.
• 6. GULEWICZ K., TROJANOWSKA K. Suppressive effect of preparations obtained from bitter lupine straw against plant pathogenic fungi. Sci. Legum. 2, 141, 1995.
• 7. HATHOUT Y., HO Y-P., RYZHOV V, DEMIREV P., FENSELAU C. Kurstakins: a new class of lipopeptides isolated from Bacillus thuringiensis. J. Natur. Product. 63,1492, 2000.
• 8. MOYNE A. L., SHELBY R., CLEVELAND T. E., TUZUN S. Bacillomycin D: an iturin with antifungal activity against Aspergillus flavus. J. Appl. Microbiol. 90, 622, 2001.
• 9. MULLER H. M, BOLEY A. Untersuchngen zur Kiih- llagerung von Weizen. Arch. Anim. Nutr. 42, 351, 1992.
• 10. NEWELL S. Y., MILLER J. D., FALLON R. D. Ergosterol content of saltmarsh fungi: effect of growth conditions and mycelial age. Mycol. 79, 688, 1987.
• 11. OERKE C, DEHNE H. W, SCHONBECK F., WEBER A. Crop protection and crop production. Elsevier, Amsterdam, 1994.
• 12. PADGETT D. E., POSEY M. H. An evaluation of the efficiencies of several ergosterol extraction techniques. Mycol. Res. 97, 1476, 1993.
• 13. PODILE A. R., PARKASH A. P. Lysis and biological control of Aspergillus niger by Bacillus subtilis AF 1. Can. J. Microbiol. 42, 533, 1996.
• 14. SEITZ L. M., MOHR H. E, BURROUGHS R., SAUER D. B. Ergosterol as fungal invasions in grains. Cereal Chem. 54, 1207,1977.
• 15. TROJANOWSKA K., GULEWICZ K., STACHOWIAK B. Identification of bacterialstrain isolated from lupine com posts and their fungistatic activity. Proc. Symp. Lupine in Modern Agriculture, Olsztyn, Poland, 257, 1997.
• 16. WORTHINGTON P. A. Antibiotics with antifungal and antibacterial activity against plant diseases. Nat. Prod. Rep. 5, 47, 1988.