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The nucleotide sequence of a chromosomal DNA fragment located upstream from the cysPTWAM operon of Escherichia coli was established. Sequence analysis indicates the presence of an open reading frame which has been designated ucpA (upstream cysE). The potential protein product exhibits strong sequence homology to the members of a large protein family, short-chain dehydrogenases/reductases. Involvement of Crp, FruR and IHF in the regulation of ucpA transcription in vivo was demonstrated.
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p.153-157,fig.
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- Polish Academy of Sciences, Pawinskiego 5a, 02-106 Warsaw, Poland
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Bibliografia
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- 12. Kolb, A., Busby, S., Buc, H., Garges, S. & Adhya, S. (1993) Annu. Rev. Biochem. 62, 749-795.
- 13. Ramseier, T.M., Bledig, S., Michotey, V., Feghali, R. & Saier, M.H., Jr. (1995) Mol. Microbiol. 16, 1157-1169.
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- 16. Miller, J.H. (1972) Experiments in Molecular Genetics; Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
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Bibliografia
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bwmeta1.element.agro-article-3d2dbedb-36d6-4e25-af4e-0ef95110432b