Probe generated by Y chromosome microdissection in useful for analysing the sex chromosomes of the domestic horse

• Autorzy: Pienkowska-Schelling A. , Bugno M. , Owczarek-Lipska M. , Schelling C. , Slota E.
• Języki publikacji: EN

Abstrakty

EN

Microdissection of chromosomes is an invaluable tool for physical isolation of single chromosomes, chromosome arms or chromosome bands. In the present study we have generated a fluorescence in situ hybridization (FISH) probe from the microdissected Y chromosome of the domestic horse. For the identification of the Y chromosome, we used the CBG technique. A biotinlabelled probe in the FISH technique gave a specific signal on all Y chromosomes and an additional signal in the interstitial part of the long arm of the X chromosome. This area showed an additional C band detected by the CBG method and characteristic for this chromosome. The probe obtained can therefore be used for analysis of the Y and X chromosomes and their copies in the genome, and, in the case of the X chromosome, also for gene mapping.

• Wydawca: -
• Czasopismo: Journal of Animal and Feed Sciences
• Rocznik: 2006
• Tom: 15
• Numer: 2
• Strony: 173-178
• Opis fizyczny: p.173-178,fig.,ref.

Twórcy

autor

Pienkowska-Schelling A.

• August Cieszkowski Agricultural University, Wolynska 33, 60-637 Poznan, Poland

autor

Bugno M.

autor

Owczarek-Lipska M.

autor

Schelling C.

autor

Slota E.

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