Enterotoksyczne szczepy Clostridium perfringens a sporulacja

• Autorzy: Augustynowicz E. , Gzyl A. , Slusarczyk J.
• Języki publikacji: PL

Abstrakty

PL

Do niedawna przeważał pogląd, że tylko sporulujące komórki Clostridium perfringens typu A zdolne są do wytwarzania enterotoksyny (CPE). Obecnie pojawia się coraz więcej doniesień wskazujących na rozłączność procesów sporulacji oraz syntezy białka CPE. Celem pracy było określenie w jakim stopniu synteza białka CPE związana jest z formowaniem spor. Enterotoksynę wykrywano po indukowaniu sporulacji metodą serologiczną z wykorzystaniem testu RPLA oraz metodami opartymi na amplifikacji na poziomie DNA oraz mRNA. Uzyskane wyniki wskazują, że sporulacja nie jest niezbędnym czynnikiem ekspresji genu cpe a wpływa jedynie na znaczny wzrost poziomu wytwarzania toksyny.

EN

Ciosiridium perfringens strains of A type capable of enterotoxin (CPE) synthesis may be a potential source of food-poisoning. Suitability of methods for CPE detection on the protein level is limited by difficulties in inducing sporulation in vitro. A number of unknown facts concerning coregulation the sporulation processes and CPE synthesis are recognised. The goal of the work was to determine the level of correlation between CPE synthesis and spores formation. Enterotoxin and cpe gen were detected by RPLA after sporulation induction test and by methods based on amplification on the DNA and mRNA levels. Sixty-four C. perfringens strains of A type isolated from patients with food poisoning symptoms and from food samples were analysed. Collection of isolates was differentiated as not enterotoxic, enterotoxic, and potentially enterotoxic strains based on appropriate strain profile: plc(+), cpe(-), CPE(-); plc(+), ;cpe(+), CPE(+); and plc (+), cpe(-), CPE(-), respectively. No significant difference between expression of cpe mRNA in vegetative and sporulation phase was found. The obtained results indicate that sporulation is not an essential factor for cpe gene expression.

Słowa kluczowe

PL

sporulacja; szczepy bakteryjne; mikrobiologia lekarska; test RPLA; zatrucia pokarmowe; Clostridium perfringens

• Wydawca: -
• Czasopismo: Medycyna Doświadczalna i Mikrobiologia
• Rocznik: 2002
• Tom: 54
• Numer: 1
• Strony: 55-60
• Opis fizyczny: s.55-60,tab.,bibliogr.

Twórcy

autor

Augustynowicz E.

• Panstwowy Zaklad Higieny, Warszawa

autor

Gzyl A.

autor

Slusarczyk J.

Bibliografia

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