Badania transmisji szczepow Haemophilus influenzae typu b krazacych w populacji dzieci w przedszkolach i domach dziecka

• Autorzy: Augustynowicz E. , Nowaczek A. , Szenborn L. , Banys D. , Gzyl A. , Dabrowski W. , Samet A. , Slusarczyk J.

Warianty tytułu

EN

Haemophilus influenzae type b strains transmission among children attending day-care canters and orphanages

• Języki publikacji: PL

Abstrakty

PL

Szczepy H. influenzae wytwarzające otoczkę polisacharydową, odmiennie do szczepów bezotoczkowych stanowią zwartą klonalnie grupę. Celem pracy było określenie poziomu zmienności szczepów H. influenzae typu b izolowanych od zdrowych dzieci z przedszkoli oraz domów dziecka oraz szczepów izolowanych z zakażeń inwazyjnych. W pracy oceniono przydatność metody PFGE w badaniach transmisji szczepów Hib krążących w populacji oraz dochodzeniach epidemiologicznych w obrębie trzech wykrytych ognisk zakażenia inwazyjnego szczepami Hib.

EN

Goal of the work was to evaluate the differences level among H. influenzae strains of b serotype isolated from children attending day-care centres and orphanages and among strains isolated from invasive infections. In the work PFGE in Hib strains transmission examination and for epidemiological studies among three sources of invasive infection was applied. Among 35 Hib strains tested and control strain 8 different pulsotyped were found. Among 21 strains colonising the nasopharynx of healthy children, and among 13 Hib strains isolated from cerebrospinal fluid, 6 and 1 pulsotypes were fund, respectively. Results obtained show that healthy children might be colonizing with genotypes characteristic for Hib strains isolated from invasive infections. In this view wider Hib vaccination seems be expected, as Hib circulation is common.

Słowa kluczowe

PL

choroby czlowieka; infekcja bakteryjna; dzieci; domy dziecka; przedszkola; szczepy bakteryjne; drogi oddechowe; transmisja; Haemophilus influenzae; metoda PFGE; epidemiologia

• Wydawca: -
• Czasopismo: Medycyna Doświadczalna i Mikrobiologia
• Rocznik: 2005
• Tom: 57
• Numer: 1
• Strony: 39-49
• Opis fizyczny: s.39-49,rys.,tab.,bibliogr.

Twórcy

autor

Augustynowicz E.

• Panstwowy Zaklad Higieny w Warszawie, ul.Chocimska 24, 00-791 Warszawa

autor

Nowaczek A.

autor

Szenborn L.

autor

Banys D.

autor

Gzyl A.

autor

Dabrowski W.

autor

Samet A.

autor

Slusarczyk J.

Bibliografia

• 1. Augustynowicz E, Gzyl A, Szenborn L i inni. Comparison of usefulness of randomly amplified polymorphic DNA and amplified - fragment length polymorphism techniques in epidemiological studies on the nasopharyngeal carriage of non-typable Haemophilus influenzae. J Med Microbiol 2003; 52: 1005-14.
• 2. Campos J, Hernando M, Roman F i inni. Analysis of invasive Haemophilus influenze infections after extensive vaccination against H. influenzae type b. J Clin Microbiol 2004; 42: 524-9.
• 3. Cerquetti M, Ciofl ML, Renna G i inni. Characterization of non-type b Haemophilus influenzae strains isolated from patients with invasive disease. J Clin Microbiol 2000; 38: 4649-52.
• 4. Curran R, Hardie KR, Towner KJ. Analysis by pulsed-field gel electrophoresis of insertion mutations in the transferring-binding system of Haemophilus influenzae type b. J Med Microbiol, 1994; 41: 120-6.
• 5. Ewald P. W. Evolution of infectious diseases. Oxford University Press, New York, N Y. 1994.
• 6. Falla TJ, Crook DWM, Brophy LN i inni. PCR for capsular typing of Haemophilus influenzae. J Clin Microbiol 1994; 32: 2382-6.
• 7. Fuste M.C, Pineda A, Palomar J i inni. Clonality of multidrug-resistant nontypeable strains of Haemophilus influenza. J Clin Microbiol 1996; 34: 2760-5.
• 8. Gazagne L, Delmas C, Bingen E i inni. Molecular epidemiology of ampicillin resistant non- b-lactamase-producing Haemophilus influenzae. J Clin Microbiol 1998; 36: 3629-35.
• 9. Gilsdorf JR. Antigenic diversity and gene polymorphisms in Haemophilus influenzae. Infect Immun 1998; 66: 5053-9.
• 10. Hargreaves RM, Slack МРЕ, Howard AJ i inni. Changing patterns of invasive Haemophilus influenzae disease in England and Wales after introduction of the Hib vaccination programme. British Med J 1996; 312: 160-61.
• 11. Meats E, Feil EJ, Stringer S i inni. Characterization of encapsulated and nonencapsulated Haemophilus influenzae and determination of phylogenic relationships by multilocus sequence typing. J Clin Microbiol 2003; 41: 1623-36.
• 12. Mitsuda T, Kuroki H, Ishikawa N i inni. Molecular epidemiological study of Haemophilus influenzae serotype b strains obtained from children with meningitis in Japan. J Clin Microbiol 1999; 37: 2548-52.
• 13. Moor PE, Collignon PC, Gwendolyn LG. Pulsed-field gel electrophoresis used to investigate genetic diversity of Haemophilus influenzae type b isolates in Australia shows differences between Aboriginal and non-Aboriginal isolates. J Clin Microbiol 1999; 37: 1524-31.
• 14. Musser JM, Kroll JS, Granoff DM i inni. Global genetic structure and molecular epidemiology of encapsulated Haemophilus influenzae. Rev Infect Dis 1990; 12: 75-111.
• 15. Musser JM, Kroll JS, Moxon ER i inni. Clonal population structure of encapsulated Haemophilus influenzae. Infect. Immun.1988; 56: 1837-45.
• 16. Nowaczek A, Augustynowicz E, Ślusarczyk J. Badanie polimorfizmu genetycznego szczepów Haemophilus influenzae izolowanych od zdrowych dzieci z wykorzystaniem reakcji amplifikacji. Med Dośw Mikrobiol 2003; 55: 231-43.
• 17. Peltola H. Worldwide Haemophilus influenzae type b disease at the beginning of the 21s1 century: global analysis of the disease burden 25 years after the use of the polysaccharide vaccine and a decade after the advent of conjugates. Clin Microbiol Rev 2000; 13: 302-17.
• 18. Saito M, Umeda A, Yoshida S. Subtyping of Haemophilus influenzae strains by PFGE. J Clin Microbiol 1999; 37: 2142-7.
• 19. Smith-Vaughan HC, Kadaba S, Sriprakash S i inni. Low genetic diversity of Haemophilus influenzae type b compared to nonencapsulated H. influenzae in a population in which H. influenzae is highly endemic. Infect Immun 1998; 66: 3403-9.
• 20. Szenborn L, Banyś D, Żarczyńska H i inni. Nosicielstwo Haemophilus influenzae b w jamie nosowo-gardłowej u małych dzieci. Pediatria Polska 1998; 73: 859-64.
• 21. Tenover FC, Arbeit RD, Goering RV i inni. Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol 1995; 33: 2233-9.
• 22. Urwin G, Krohn JA, Deaver-Robinson K i inni. Invasive disease due to Haemophilus influenzae serotype f: clinical and epidemiologic characteristics in the H. influenzae serotype b vaccine era. Clin Infect Dis 1996; 22: 1069-76.