The reproductive toxicity of the organophosphate pesticide O,O-dimethyl 0-4-nitrophenyl phosphorothioate [methyl parathion] in the male rat

• Autorzy: Prashanthi N , Narayana K. , Nayanatara A. , Chandra Kumar H.H. , Bairy K.L. , D'Souza U.J.A.
• Języki publikacji: EN

Abstrakty

EN

Methyl parathion (MP) is a pesticide widely used to protect crops but also illegally used in many countries for spraying homes and businesses to contain insects. The present study was planned to investigate the effects of MP on the male reproductive organs in the rat. Male Wistar rats (13–14 weeks old) were treated with MP and sacrificed as follows. Experiment 1: 0 (water vehicle), 1.75, 3.5 or 7 mg/kg (i.p.) for 5 days and sacrificed on day 14; experiment 2: 0, 0.5 or 1 mg/kg (i.p.) for 12 days and sacrificed on day 130; experiment 3: 0, 0.5 or 1 mg/kg (i.p.) for 12 days and sacrificed on day 77; experiment 4: 0, 0.75 or 1.5 mg/kg (i.p.) for 25 days and sacrificed on day 17; experiment 5: 0 or 3.5 mg/kg (p.o.) for 25 days and sacrificed on day 17 after the last exposure. The reproductive organs were removed, weighed and processed for histopathological analysis. Structural changes, for example the morphology of the epithelium and the lumina of the organs, were observed in all animals. Biochemical estimates of acid phosphatase (ACP), cholesterol, total protein, uric acid, and vitamin C were conducted in the epididymes. The weight of the epididymes increased in experiment 2 in a dose-dependent pattern (p < 0.01) and decreased in experiments 4 and 5 (p < 0.01). The weight of the ductus deferens decreased in experiment 3 at 1 mg/kg dose level (p < 0.001) and increased in experiment 5 (p < 0.05). The weight of the seminal vesicle decreased in experiment 3 at both 0.5 mg/kg and 1 mg/kg dose levels (p < 0.001), and increased in experiment 5 (p < 0.01). The weight of the prostate decreased in experiments 4 (in a dose-dependent pattern) and 5 (p < 0.001). ACP levels decreased in experiment 4 (p < 0.001) with a greater effect at 0.5 mg/kg than at 1 mg/kg. In experiment 5 (p < 0.01) cholesterol levels decreased to less than 50% of the control level for this experiment (p < 0.01) and protein levels also decreased (p < 0.01). Vitamin C levels decreased in a dose-dependent pattern in experiments 4 (p < 0.001) and 5 (p < 0.01). There were no effects on uric acid level. Sperm density was decreased in the epididymes of the rats treated and the epithelium of the epididymis and ductus deferens showed cellular necrosis, brush-border disruption and nuclear pyknosis. Nuclei were haloed, except in experiment 2 and the 0.5 mg/kg group of experiment 3. Methyl parathion did not induce significant changes in the structure of the seminal vesicle and prostate, except that epithelial folding was shorter than in the control. In conclusion, MP is a reproductive toxicant in the male rat and causes deterioration in the structural integrity of the reproductive organs and also the biochemical parameters in the epididymis.

Słowa kluczowe

EN

reproductive toxicity; organophosphate pesticide; o,o-dimethyl o-4-nitrophenyl phosphorothioate; pesticide; gonadal toxicity; fertility; endocrine disruptor; male; rat

• Wydawca: -
• Czasopismo: Folia Morphologica
• Rocznik: 2006
• Tom: 65
• Numer: 4
• Strony: 309-321
• Opis fizyczny: p.309-321,fig.,ref.

Twórcy

autor

Prashanthi N

• Kasturba Medical College, Bejai, Mangalore, Karnataka, India

autor

Narayana K.

autor

Nayanatara A.

autor

Chandra Kumar H.H.

autor

Bairy K.L.

autor

D'Souza U.J.A.

Bibliografia

• 1. Abraham P, Wilfred G (2000) Lysosomal enzymes in the pathogenesis of carbon tetrachloride induced injury to the kidney and testis in the rat. Indian J Pharmacol, 32: 250–251.
• 2. Asmathbanu I, Kaliwal BB (1997) Temporal effect of methyl parathion on ovarian compensatory hypertrophy, follicular dynamics and estrous cycle in hemicastrated albino rats. J Basic Clin Physiol Pharmacol, 8: 237–254.
• 3. Barr DB, Allen R, Olsson AO, Bravo R, Caltabiano LM, Montesano A, Nguyen J, Udunka S, Walden D, Walker RD, Weerasekera G, Whitehead Jr RD, Schober SE, Needham LL (2005) Concentrations of selective metabolites of organophosphorus pesticides in the United States population. Environ Res, 99: 314–326.
• 4. Bhatnagar VK (2001) Pesticide pollution: Trends and perspective. ICMR Bulletin, 31: 1–9.
• 5. Chinoy MR, Sharma JD, Sanjeevan AG, Chinoy NJ (1983) Structural changes in male reproductive organs and spermatozoa of scorbutic guinea-pigs. Proc Ind Natr Sci Acad, B49: 628–635.
• 6. Chitra KC, Rao KR, Mathur PP (2003) Effect of bisphenol A and co-administration of bisphenol A and vitamin C on epididymis of adult rats: A histological and biochemical study. Asian J Androl, 5: 203–208.
• 7. Culling CFA, Allison RT, Barr WT (1985) Cellular pathology technique, 4th Edition. Butterworths, London, pp. 27–50.
• 8. D’Souza UJA, Narayana K (2002) Formation, morphology and fate of multinucleated cells (symplasts) in the rat testis exposed to 5-fluorouracil. Indian J Physiol Pharmacol, 46: 504–506.
• 9. D’Souza UJA, Narayana K (2001) Induction of seminiferous tubular atrophy by single dose of 5-fluorouracil (5-FU) in Wistar rats. Indian J Physiol Pharmacol, 45: 87–94.
• 10. De Aguiar LH, Moraes G, Avilez IM, Altran AE, Correa CF (2004) Metabolical effects of Folidol 600 on the neotropical freshwater fish mantrinxa Brycon cephalus. Environ Res, 95: 224–230.
• 11. Dhondup P, Kaliwal BB (1997) Inhibition of ovarian compensatory hypertrophy by the administration of methyl parathion in hemicastrated albino rats. Reprod Toxicol, 11: 77–84.
• 12. Eskenazi B, Bradman A, Castorina R. (1999) Exposure of children to organophosphate pesticides and their potential adverse health effects. Environ Health Perspect, 107 (suppl 3): 409–419.
• 13. Gupta RC, Thornburg JF, Stedman DB, Welsch F (1984) Effect of subchronic administration of methyl parathion on in vivo protein synthesis in pregnant rats and their conceptuses. Toxicol Appl Pharmacol, 92: 457-468.
• 14. Gupta RC, Rech RH, Lovell KL, Welch F, Thornburg JE (1985) Brain cholinergic, behavioral, and morphological development in rats exposed in utero to methyl parathion. Toxicol Appl Pharmacol, 77: 405–413.
• 15. Hess RA, Linder RE, Strader LF, Perreault SD (1988) Acute effects and long-term sequelae of 1, 3-dinitrobenzene on male reproduction in the rat II. Quantitative and qualitative histopathology of the testis. J Androl, 9: 327–342.
• 16. Institoris L, Papp A, Siroki O, Banerjee BD (2004) Comparative investigation of behavioral, neurotoxicological, and immunotoxicological indices in detection of subacute combined exposure with methyl parathion and propoxur in rats. Ecotoxicol Environ Saf, 57: 270–277.
• 17. IPCS (International Programmme on Chemical Safety) (1993) Environmental Health Criteria 145, Methyl Parathion, WHO, pp. 11–236.
• 18. Jana D, Maiti R, Gosh D (2003) Effects of Stephania hernandifolia leaf extract on testicular activity in rats. Asian J Androl, 5: 125–129.
• 19. Jelinek R, Peterka M, Rychter Z (1985) Chick embryo toxicity screening test-130 substances tested. Indian J Exp Biol, 23: 588–595.
• 20. Kaplan LA, Pesce AJ (1987) Vitamins. In: Clinical chemistry. 2nd ed, pp 543–568.
• 21. Kaur S, Dhanju CK (2005) Biochemical effects of some organophosphorus pesticides on the ovaries of albino rats. Indian J Physiol Pharmacol, 49: 148–152.
• 22. Kramer RE, Wellman SE, Zhu H, Rockhold RW, Baker RC (2002) A comparison of cholinesterase activity after intravenous, oral or dermal administration of methyl parathion. J Biomed Sci, 9: 140–148.
• 23. Kumar KB, Devi KS (1992) Teratogenic effects of methyl parathion in developing chick embryos. Vet Hum Toxicol, 34: 408–410.
• 24. Latchoumycandane C, Chitra KC, Mathur PP (2002) The effect of methoxychlor on the epididymal antioxidant system of adult rats. Reprod Toxicol, 16: 161–172.
• 25. Levario-Carrillo M, Olave ME, Corral DC, Alderete JG, Gagioti SM, Bevilacqua E (2004) Placental morphology of rats prenatally exposed to methyl parathion. Exp Toxicol Pathol, 55: 489–496.
• 26. Loeffler IK, Peterson RE (1994) Interactive effects of TCDD and p, p’-DDE on male reproductive tract development in in utero and lactationally exposed rats. Toxicol Appl Pharmacol, 154: 28–39.
• 27. Ma T, Kramer RE, Baker RC, Fan LW, Ho IK (2003) Effects of chronic dermal exposure to nonlethal doses of methyl parathion on brain regional acetylcholinesterase and muscarinic cholinergic receptors in female rats. J Neurosci Res, 71: 138–145.
• 28. Maitra SK, Sarkar R (1996) Influence of methyl parathion on gametogenic and acetylcholinesterase activity in the testis of white-throated munia (Lonchura malabarica). Arch Environ Contam Toxicol, 30: 384–389.
• 29. Makita Y, Omura M, Tanaka A, Kiyohara C (2005) Effects of concurrent exposure to tributyltin and 1, 1-dichloro-2, 2 bis (p-chlorophenyl) ethylene (p, p’-DDE) on immature male Wistar rats. Basic Clin Pharmacol Toxicol, 97: 364–368.
• 30. Manna SK, Zhang HJ, Yan T, Oberley LW, Aggarwal BB (1998) Overexpression of manganese superoxide dismutase suppresses tumor necrosis factor-induced apoptosis and activation of nuclear transcription factor-kappa B and activated protein-1. J Biol Chem, 273: 13245–13254.
• 31. Mathew G, Vijayalaxmi KK, Rahiman MA (1992) Methyl parathion-induced sperm shape abnormalities in mouse. Mutation Res, 280: 169–173.
• 32. Narayana K, Prashanthi N, Nayanatara A, Kumar HHC, Abhilash K, Bairy KL (2005) Effects of methyl parathion (o, o-dimethyl o-4-nitrophenyl phosphorothioate) on rat sperm morphology and sperm count, but not fertility, are associated with decreased ascorbic acid level in the testis. Mutation Res, 588: 28–34.
• 33. Naslund MJ, Coffey DS (1986) The differential effects of neonatal androgens, estrogens and progesterons on adult rat prostate growth. J Urol, 136: 1126–1140.
• 34. Niu Y, Ma T-X, Zhang J, XU Y, Han RF, Sun G (2003) Androgen and prostatic stroma. Asian J Androl, 5: 19–26.
• 35. Okazaki K, Okazaki S, Nishimura S, Nakamura H, Kitamura Y, Hatayama K, Nakamura A, Tsuda T, Katsumata T, Nishikawa A, Hirose M (2001) A repeated 28-day oral dose toxicity study of methoxychlor in rats, based on the «enhanced OECD test guideline 407» for screening endocrine-disrupting chemicals. Arch Toxicol, 75: 513–521.
• 36. Pant N, Srivastava SP (2003) Testicular and spermatotoxic effects of quinalphos in rats. J Appl Toxicol, 23: 271–274.
• 37. Poon R, Rigden R, Chu I, Valli VE (2004) Short-term oral toxicity of pentyl ether, 1, 4-diethoxybutane, and 1, 6-dimethoxyhexane in male rats. Toxicol Sci, 77: 142–150.
• 38. Roy S, Banerjee A, Pandey HC, Singh G, Kumari GL (2001) Application of seminal germ cell morphology and semen biochemistry in the diagnosis and management of azoospermic subjects. Asian J Androl, 3: 55–62.
• 39. Rubin C, Esteban E, Hill Jr RH, Pearce K (2002) Introduction — the methyl parathion story: a chronicle of misuse and preventable human exposure. Environ Health Perspect, 110 (suppl 6): 1037–1040.
• 40. Ruckart PZ, Kakolewski K, Bove FJ, Kaye WE (2004) Long-term neurobehavioral health effects of methyl parathion exposure in children in Mississippi and Ohio. Environ Health Perspect, 112: 46–51.
• 41. Sortur SM, Kaliwal BB (1999) Effect of methyl parathion formulation on estrous cycle and reproductive performances in albino rats. Indian J Exp Biol, 37: 176–178.