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2006 | 09 | 1 |
Tytuł artykułu

Citric acid biosynthesis by Yarrowia lipolytica A-101-1.31 under deficiency of various medium macrocomponents

Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
The objective of the study was to examine the potential of Yarrowia lipolytica A-101-1.31 growth control via the concentration of the nitrogen, phosphorus or sulphur source, as well as to assess the efficiency of each source separately and under simultaneous deficiency of nitrogen and phosphorus. The experimental study has produced the following findings: nitrogen, phosphorus and sulphur enabled the control of Y.lipolytica A-101-1.31 growth. Citric acid biosynthesis occurred irrespective of the growth-limiting factor used. The yeast cells produced citric acid with the desired efficiency only under conditions of nitrogen (qCAg=0.071 gg-1h-1) or sulphur (YCAP=0.74 gg-1) deficiency. The smallest changes in the elemental composition were observed when the yeast grew under nitrogen limitation. Elemental composition variations were the most distinct when the yeast biomass was cultivated under phosphorus deficiency. A low deficiency of phosphorus in the nitrogen deficient had a stimulating effect on the acid-producing of the growing cells (qCAg=0.089 gg-1h-1).
Wydawca
-
Rocznik
Tom
09
Numer
1
Opis fizyczny
http://www.ejpau.media.pl/volume9/issue1/art-15.html
Twórcy
autor
  • Wroclaw University of Environmental and Life Sciences, Norwida 25, 50-373 Wroclaw, Poland
autor
Bibliografia
  • Anastassiadis S., Aivasidis A., Wanddrey C., 2002. Citric acid production by Candida strains under intracellular nitrogen limitation. Appl. Microbiol. Biotechnol. 60, 81-87.
  • Antonucci S., Bravi M., Bubbico R., Di Michele A., Verdone N., 2001. Selectivity in citric acid production by Yarrowia lipolytica. Enzyme and Microbial Technology, 28, 189-195.
  • Arzumanov T. E., Shishkanova N. V., Finogenova T. V., 2000. Biosynthesis of citric acid by Yarrowia lipolytica repeat-batch on culture on ethanol. Appl. Microbiol. Biotechnol. 53 (5), 525-529.
  • Bubbico R., Presti S., Bravi M., Moresi M., Spinosi M., 1996. Repeated batch citrate production by Yarrowia lipolytica using yeast recycling by cross-flow microfiltration. Agro Food-Ind. Hi-Tech, 3, 35-38.
  • Enzminger J. D., Asejno J. A., 1986. Use of cell recycle in the aerobic fermentative production of citric acid by yeast. Biotechnol. Letters., 8 (1), 7-12.
  • Furukawa T., Ogino T., Matsuyoshi T., 1982. Fermentative production of citric acid from n-paraffins by Saccharomycopsis lipolytica. J. Ferment. Technol., 60 (4), 281-286.
  • Goldberg D., Ellis G., 1983. Isocitrate dehydrogenase. In: Bergmeyer HU (ed) Methods of enzmatic analysis. Verlag Chemie, Weinheim, 3, 183-190.
  • Ikeno Y., Masuda M., Tanno K., Oomori I., Takahashi N., 1975. Citric acid production from various raw materials by yeasts, J. Ferment. Technol., 53 (10), 752-756.
  • Klasson T. K., Clausen E. C. Gaddy J. L., 1989. Continuous fermentation for the production of citric acid from glucose. Appl. Biochem. Biotechnol., 20/21, 491-509.
  • Maddox I. S., Kingston P. J., 1983. Use of Immobilized Cells of the Yeast Saccharomycopsis lipolytica for the Production of Citric Acid. Biotechnol. Letters, 5 (12), 795-798.
  • McKay I. A., Maddox I. S., Brooks J. D., 1994. High specific rates of glucose utilization under conditions of restricted growth are required for citric acid accumulation by Yarrowia lipolytica IMK 2, Appl. Microbiol. Biotechnol. 41, 73-78.
  • Rane K. D., Sims K., 1995. Citric Acid Production by Candida lipolytica Y 1095 in Cell Recycle and Fed-Batch Fermentations. Biotechnol. Bioeng., 46, 325-332.
  • Rane K. D., Sims K., 1993. Production of citric acid by Candida lipolytica Y1095: Effect of glucose concentration on yield and productivity. Enzyme Microb. Technol., 15, 646-651.
  • Rymowicz W., 1995. Optymalizacja procesu biosyntezy kwasu cytrynowego z glukozy przez szczep Y. lipolytica A-101, [Optymization of citric acid production from glucose by strain Yarrowia lipolytica A-101]. Zeszyty Nauk. AR Tech. Żywn., 273, 7-20 [in Polish].
  • Rymowicz W., 1998. Biosynteza kwasu cytrynowego z glukozy przez wolne i immobilizowane komórki drożdży Yarrowia lipolytica w systemach cišgłych, [Biosynthesis of citric acid from glucose by free and immobilized Yarrowia lipolytica cells in continuous systems]. Rozprawa habilitacyjna, Zeszyty naukowe AR we Wrocławiu nr 329, Rozprawy CLI [in Polish].
  • Rymowicz W., Wojtatowicz M., Rywińska A., Kita A., 1996. Cišgła biosynteza kwasu cytrynowego z udziałem mutantów octanowych Yarrowia lipolytica, [The continuous citric acid production by acetate mutants of Yarrowia lipolytica]. Materiały VI Ogólnokrajowej Konferencji Naukowej, ŁódŸ 24-26.09, Referat, 13, 1-6 [in Polish].
  • Stern J., 1957. Assay of tricarboxylic acids, Methods Enzymol. 3, 425-431.
  • Wojtatowicz M., 1991. Studia nad biosyntezš kwasu cytrynowego przez szczep drożdży Yarrowia lipolytica A-101 i jego mutanty, [Study on citric acid biosynthesis by yeast strain of Yarrowia lipolytica A-101 and its mutants]. Zeszyty Nauk. AR Wrocław Rozpr. Hab., 96 [in Polish].
  • Wojtatowicz M., Rymowicz W., 1995. Produktywnosc i wydajnosc drożdżowej fermentacji cytrynowej w różnych systemach hodowlanych, Biotechnol, [Volumetric citric acid productivity and field of citrate biosynthesis by yeast in different cultivation systems]. 2 (29), 123-129 [in Polish].
  • Żarowska B., Wojtatowicz M., Rymowicz W., Robak M., 2001. Production of citric amid on sugar beet molasses by single and mixed cultures of Yarrowia lipolytica. Electronic Journal of Polish Agricultural Universities. 4, 2, series Biotechnology, 1-8.
  • Żarowska B., 1999. Biosynteza kwasu cytrynowego na melasie przez drożdże Yarrowia liplytica, [Citric acid biosynthesis on sugar beet molasses by the yeast Yarrowia lipolytica]. Rozprawa doktorska, Akademia Rolnicza Wrocław [in Polish].
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-article-37edc15c-e5e4-42f0-9623-061c15453e75
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