Prevalence of infection with Francisella tularensis, Borrelia burgdorferi sensu lato and Anaplasma phagocytophilum in rodents from an endemic focus of tularemia in Bulgaria

• Autorzy: Christova I. , Gladnishka T.
• Języki publikacji: EN

Abstrakty

EN

The prevalence of Francisella tularensis, Borrelia burgdorferi and Anaplasma phagocytophilum in rodents is a determinant for their role in maintaining pathogens in the environment. A total of 169 rodents, trapped in an endemic focus of tularemia, were examined by PCR to asses the frequency of infection with the etiological agents of tularemia, Lyme borreliosis and human granulocytic anaplasmosis. The overall prevalence of F. tularensis was 22%. In 6% of the black rats, F. tularensis was identified in coinfection with B. burgdorferi and in 3% in coinfection with A. phagocytophilum. B. burgdorferi and A. phagocytophilum were detected in 26% and 8% of the trapped rodents. Results obtained show the existence of an active natural focus of tularemia. The high level of coinfections indicated that the endemic focus is mixed and generates risk for multiple infections in humans. Further investigations are needed to reveal interactions between the pathogens in the infected animals.

Słowa kluczowe

EN

prevalence; infection; Francisella tularensis; tularemia; Anaplasma phagocytophilum; polymerase chain reaction; Borrelia burgdorferi; rodent; Bulgaria

• Wydawca: -
• Czasopismo: Annals of Agricultural and Environmental Medicine
• Rocznik: 2005
• Tom: 12
• Numer: 1
• Opis fizyczny: p.149-152,ref.

Twórcy

autor

Christova I.

• National Centre of Infectious and Parasitic Diseases, Sofia, Bulgaria

autor

Gladnishka T.

Bibliografia

• 1. Aeschlimann A, Chamot E, Gigon F, Jeanneret J-P, Kesseler D, Walther C: В. burgdorferi in Switzerland. Zbi Bakt Hyg A 1986, 262, 450-458.
• 2. Berdal B, Mehl R, Meidell N, Lorentzen-Styr A-M, Scheel O: Field investigations of tularemia in Norway. FEMS Immunol Med Microbiol 1996,13, 191-195.
• 3. Bunnel J, Dumler J, Childs J, Glass G: Retrospective serosurvey for human granulocytic ehrlichiosis agent in urban white-footed mice from Maryland. J Wildl Dis 1998, 34, 179-181.
• 4. Guryčová D, Kocianová E, Výrosteková V, Řeháček J: Prevalence of ticks infected with Francisella tularensis in natural foci of tularemia in western Slovakia. Eur J Epidemiol 1995,11, 469-474.
• 5. Guryčová D, Výrosteková V, Khanakah G, Kocianová E, Stanek G: Importance of surveillance of tularemia natural foci in the known endemic area of Central Europe. Wien Klin Wochenschr 2001, 113, 433- 438.
• 6. Hopla CE: The ecology of tularemia. Adv Vet Sci Comp Med 1974, 18, 25-53.
• 7. Hu C, Humaire P, Wallich R, Gern L: Apodemus sp. rodents, reservoir hosts for Borrelia afzelii in an endemic area of Switzerland. Zentralbl Bakteriol 1997, 285, 558-564.
• 8. Humair P, Rais O, Gern L: Transmission of Borrelia afzelii from Apodemus mice and Clethrionomys voles to Ixodes ricinus ticks: differential transmission pattern and overwintering maintenance. Parasitology 1999,118, 33-42.
• 9. Liz J, Anderes L, Sumner J, Massung R, Gern L, Rutti В, Brossard: PCR detection of granulocytic ehrlichiae in Ixodes ricinus ticks and wild small mammals in western Switzerland. J Clin Microbiol 2000, 38, 1002-1007.
• 10. Magnarelli L, Anderson J, Stafford III K, Dumler J: Antibodies to multiple tick-borne pathogens of babesiosis, ehrlichiosis, and Lyme borreliosis in white-footed mice. J Wildl Dis 1997, 33, 466-473.
• 11. Matuschka F-R, Endepolis A, Richter D, Spielman A: Competence of urban rats as reservoir hosts for Lyme disease spirochetes. J Med Entomol 1997, 34, 489-493.
• 12. Matuschka F-R, Fischer P, Heiler M, Richter D, Spielman A: Capacity of European animals as reservoir hosts for the Lyme disease spirochete. J Infect Dis 1994,165, 479-483.
• 13. Могпег T: The ecology of tularemia. Rev Sei Tech Off Int Epiz 1992,11, 1123-1130.
• 14. Ogden N, Bown K, Horrocks B, Woldehiwet Z, Bennett M: Granulocytic Ehrlichia infection in ixodid ticks and mammals in woodlands and uplands of the UK. Med Vet Entomol 1998, 12, 423-429.
• 15. Pearson AP: Tularemia. In: Palmer SR, Lord Soulsby, Simpson DIH (Eds): Zoonoses: biology, clinical practice, and public health control, 267-280. Oxford University Press, Oxford 1998.
• 16. Randolph S, Craine N: General framework for comparative quantitative studies on transmission of tick-borne diseases using Lyme borreliosis in Europe as an example. J Med Entomol 1995, 2, 765-777.
• 17. Reintjes R, Dedushaj I, Gjini A, Jorgenses T, Cotter B, Lieftucht A, D'Ancona F, Dennis D, Kosoy M, Mulliqi-Osmani G, Grunow R, Kalaveshi A, Gashi L, Humolli I : Tularemia outbreak investigation in Kosovo: Case control and environmental studies. Emerg Infect Dis 2002, 8, 69-73.
• 18. Sjöstedt A, Eriksson U, Berglund L, Tärnvik A: Detection of F. tularensis in ulcers of patients with tularemia by PCR. J Clin Microbiol 1997, 35, 1045-1048.
• 19. Tälleklint L, Jeanson T: Transmission of Borrelia burgdorferi s.l. from mammal reservoirs to the primary vector of Lyme borreliosis Ixodes ricinus (Acari: Ixodidae), in Sweden. J Med Entomol 1994, 31, 880-886.
• 20. Tälleklint L, Jaenson T: Is the small mammal (Clethrionomys glareolus) or the tick vector (Ixodes ricinus) the primary overwintering reservoir for the Lyme borreliosis spirochete in Sweden? L Wildl Dis 1995, 31, 537-540.
• 21. Tärnvik A, Sandstrom G, Sjöstedt A: Epidemiological analysis of tularemia in Sweden, 1931-1993. FEMS Immunol Med Microbiol 1996, 13, 201-204.
• 22. Telford S III, Dawson J, Katavolos P, Warner C, Kolbert C, Persing D: Perpetuation of the agent of human granulocytic ehrlichiosis in a deer tick-rodent cycle. Proc Natl Acad Sci USA 1996, 93, 6209- 6214.
• 23. Výrosteková V, Khanakah G, Kocianová E, Guryčová D, Stanek G: Prevalence of coinfection with F. tularensis in reservoir animals of Borrelia burgdorferi sensu lato. Wien Klin Wochenschr 2002, 14, 482- 488.
• 24. Walls J, Caturegli P, Bakken J, Asanovich K, Dumler J: Improved sensitivity of PCR for diagnosis of human granulocytic ehrlichiosis using epankl genes of Ehrlichia phagocytophila - group Ehrlichiae. J Clin Microbiol 2000, 38, 354-356.
• 25. Walls J, Greig B, Neitzel D, Dumler J: Natural infection of small mammal species in Minnesota with the agent of human granulocytic ehrlichiosis. J Clin Microbiol 1997, 35, 853-855.
• 26. Walls J, Greig B, Neitzel D, Dumler J: Natural infection of small mammal species in Minnesota with the agent of human granulocytic ehrlichiosis. J Clin Microbiol 1997, 35, 853-855.
• 27. Yeh M, Mather T, Coughlin R, Gingrich-Baker C, Sumner J, Massung R: Serologic and molecular detection of granulocytic ehrlichiosis in Rhode Island. J Clin Microbiol 1997, 35, 944-947.
• 28. Zore A, Petrovec M, Prosenc K, Trilar T, Rizuc-Sabljic E, Avsic- Zupanc T: Infection of small mammals with Borrelia burgdorferi sensu lato in Slovenia as detrmined by polymerase chain reaction (PCR). Wien Klin Wochenschr 1999, 11, 997-999.