Adrenergic and cholinergic innervation of pulmonary tissue in the pig

• Autorzy: Wojtarowicz A , Podlasz P. , Czaja K.
• Języki publikacji: EN

Abstrakty

EN

Both the adrenergic and the cholinergic components of the autonomic nervous system (ANS) have been found to be an important source of nerve fibres supplying the lungs. On the other hand, data regarding the innervation of the pulmonary tissue in breeding animals are surprisingly scarce. Therefore, in the present study noradrenergic and acetylcholinesterase-positive (AChE-positive) innervation of the lungs of sexually immature pigs was studied using histochemical methods. Studies were performed on six juvenile female pigs (aged 9 weeks, body weight 15–20 kg). Samples of the tissue were collected from the caudal lobe of the right lung. 10µm cryostat sections were processed for the sucrosepotassium phosphate-glyoxylic acid technique to determine the occurrence and distribution of noradrenergic nerve fibres, while AChE-positive nerves were detected according to the acetylcholinesterase histochemistry. The present results revealed a dense network of noradrenergic nerve fibres localised mainly in the muscular membrane surrounding the epithelium of the bronchuli while AChE- -positive nerve terminals supplied functional capillary vessels localised in the inter-alveolar septum and mucous membrane of the bronchi and bronchuli. The results of the present study confirm those of physiological experiments reporting the influence of the adrenergic and cholinergic components of the autonomic nervous system on the lung functions of pigs.

Słowa kluczowe

EN

adrenergic innervation; cholinergic innervation; pulmonary tissue; pig; lung

• Wydawca: -
• Czasopismo: Folia Morphologica
• Rocznik: 2003
• Tom: 62
• Numer: 3
• Strony: 215-218
• Opis fizyczny: p.215-218,fig.,ref.

Twórcy

autor

Wojtarowicz A

• University of Warmia and Mazury in Olsztyn, 10-957 Olsztyn, Poland

autor

Podlasz P.

autor

Czaja K.

Bibliografia

• 1. de la Torre JC, Surgeon JW (1976) Histochemical fluorescence of tissue and brain monoamines: results in 18 minutes using the sucrose-phosphate-glyoxylic acid (SPG) method. Neuroscience, 1: 451–453.
• 2. Friedman M, Wang SY, Stahl GL, Johnson RG, Sellke FW (1995) Altered beta-adrenergic and cholinergic pulmonary vascular responses after total cardiopulmonary bypass. J Appl Physiol, 79:1998–2006.
• 3. Gulbenkian S, Valenca A, Wharton J, Polak JM, David-Ferreira JF (1991) Simultaneous visualization of neuropeptide and acetylcholinesterase nerve subpopulations in the perivascular plexus. Histochem J, 23: 553–558.
• 4. Hadjokas NE, Crowley JJ, Bayer CR, Nielson CP (1995) Beta-adrenergic regulation of the eosinophil respiratory burst as detected by lucigenin-dependent luminescence. J Allergy Clin Immunol, 95: 735–741.
• 5. Ivanov LN, Undritsov MI, Gordon DS, Kreimerman GM, Mandrakov VI (1977) Adrenergic and cholinergic structures of guinea pig lungs under normal conditions and in experimental bronchial asthma. Biull Exp Biol Med, 83: 446–448.
• 6. Kraneveld AD, James DE, de Vries A, Nijkamp FP (2000) Excitatory nonadrenergic-noncholinergic neuropeptides: key players in asthma. Eur J Pharmacol, 405: 113–129.
• 7. Liebhart J, Malolepszy J, Dor A (1995) Role of the autonomic nervous system (adrenergic, cholinergic and nonadrenergic, noncholinergic) in regulation of respiratory system function. Post Hig Med Dosw, 49: 395–407.
• 8. Matran R (1991) Nervous control of bronchial circulation in pigs: application to the airway stimulation. Pathol Biol (Paris), 39: 223–229.
• 9. Richardson J, Beland J (1976) Nonadrenergic inhibitory nervous system in human airways. J Appl Physiol, 41: 764–771.
• 10. Sparrow MP, Warwick SP, Everett AW (1995) Innervation and function of the distal airways in the developing bronchial tree of fetal pig lung. Am J Respir Cell Mol Biol, 13: 518–525